Characterization of the transforming domain of a feline sarcoma virus encoding a fgr-related tyrosine kinase.

Abstract:

:We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embryo fibroblasts (CEF) were transfected and monitored for replication, integration and transcription of the proviral constructs. We demonstrated that transfected cells display morphological changes and are able to form colonies in soft-agar. This suggests that the gag portion of the fusion protein from TP1-FeSV is not necessary for the transformation of fibroblasts.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Weyrauch G,Barnekow A

doi

10.1007/BF01379113

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

141-55

issue

1-2

eissn

0304-8608

issn

1432-8798

journal_volume

134

pub_type

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