Abstract:
:We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embryo fibroblasts (CEF) were transfected and monitored for replication, integration and transcription of the proviral constructs. We demonstrated that transfected cells display morphological changes and are able to form colonies in soft-agar. This suggests that the gag portion of the fusion protein from TP1-FeSV is not necessary for the transformation of fibroblasts.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Weyrauch G,Barnekow Adoi
10.1007/BF01379113subject
Has Abstractpub_date
1994-01-01 00:00:00pages
141-55issue
1-2eissn
0304-8608issn
1432-8798journal_volume
134pub_type
杂志文章abstract::The '6K1' protein of the Pinellia isolate of Soybean mosaic virus was cloned into a prokaryotic expression vector and a polyclonal antiserum raised to the expressed fusion protein. In immunogold labeling of thin sections of infected leaves of Pinellia ternata, specific labeling occurred at the cell periphery. This mig...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0972-7
更新日期:2007-01-01 00:00:00
abstract::The plaques formed by wild-type (WT) populations of strain K485 visna (V-K485) virus, strain K796 visna (V-K796) virus, and of progressive pneumonia virus in sheep choroid plexus (SCP) cell cultures are heterogeneous in size. Plaque purification procedures showed that this heterogeneity was due to the presence of two ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318133
更新日期:1981-01-01 00:00:00
abstract::The porcine paramyxovirus LPM recognizes alpha, but not beta, anomers of sialic acid containing structures, specifically sialyl (alpha 2,3) lactose. The virus specificity is directed to the sialyl residue and to the C'4 axial OH and the C'6 CH2OH of the galactose present in this structure. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309755
更新日期:1993-01-01 00:00:00
abstract::Begomoviruses have been detected infecting the weed Cnidoscolus urens (family Euphorbiaceae) since 2004, but the viral species to which these viruses belonged was not known. Here, we report for the first time the complete genome sequence of a bipartite begomovirus obtained from C. urens collected in the state of Alago...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2919-3
更新日期:2016-09-01 00:00:00
abstract::This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1673-z
更新日期:2013-08-01 00:00:00
abstract::Human cytomegalovirus (HCMV), oxidized by sodium periodate (NaIO4), is incapable of giving rise to viral progeny in cell culture. At a NaIO4 concentration as low as 5 mM, there is a loss of at least 6 logs of viral infectivity which occurs very rapidly (less than 5 min). Further, the inactivation is a first-order reac...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309765
更新日期:1994-01-01 00:00:00
abstract::RNA was extracted from 'Hugh Dickson' rose leaves displaying virus-like symptoms in Maryland, USA. Using high-throughput sequencing, we identified a new virus, tentatively named "rose virus R". This virus has a negative-sense, single-stranded RNA genome and exhibits genomic features of a rhabdovirus, including a genom...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04927-6
更新日期:2021-01-04 00:00:00
abstract::In order to prepare H5N1 influenza virus vaccine, the hemagglutinins (HAs) of 14 H5 virus isolates from water birds in Asia were antigenically and genetically analyzed. Phylogenetic analysis of the H5 HA genes revealed that 13 isolates belong to Eurasian and the other one to North American lineages. Each of the deduce...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0226-3
更新日期:2008-01-01 00:00:00
abstract::When 66 cucurbit samples with yellowing symptoms from fields in Mali, the Philippines, Thailand and Uzbekistan were screened by RT-PCR using universal polerovirus primers, 21 were identified as harboring polerovirus RNA. When these 21 samples were screened with specific primers for the known cucurbit-infecting polerov...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1939-5
更新日期:2014-06-01 00:00:00
abstract::The mechanism of cell death induced by feline immunodeficiency virus (FIV) infection was investigated in an interleukin 2(IL-2)-dependent T-lymphoblastoid cell line (MYA-1). DNA extracted from FIV-infected MYA-1 cells showed a ladder of nucleosomal DNA, indicating that the cytopathic effect (CPE) observed in these cel...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309772
更新日期:1994-01-01 00:00:00
abstract::We constructed a recombinant baculovirus encoding a dengue (DEN)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its C-terminus (D2E delta 102). The production, processing and transportation of the recombinant protein in baculovirus-infected Spodoptera frugiperda (Sf9) cells and its immunogenic prop...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309465
更新日期:1994-01-01 00:00:00
abstract::Retrovirus-like particles were isolated from the urine of a patient with psoriasis. The major internal protein, p27, in these particles was isolated by immunosorbent chromatography and gel filtration on a Sephacryl S-300 column in 6 M guanidine hydrochloride. The protein was purified to homogeneity as judged by SDS-PA...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314231
更新日期:1985-01-01 00:00:00
abstract::The VP1 encoded by genomic segment B of birnaviruses is generally known to exist as a genome-linked protein (VPg) and as a "free" polypeptide of 90 kDa in virus particles. The guanylylation activity associated with infectious bursal disease virus (IBDV) was demonstrated by incubating purified virus in presence of [alp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050154
更新日期:1997-01-01 00:00:00
abstract::Site-directed mutagenesis was used to study the biological significance of three N-linked glycans (linked to Asn406, Asn448, and Asn463), situated in the CD4-binding region of gp120. Mutagenesis was carried out in a phage M13 system, and the mutated env genes were inserted into recombinant vaccinia virus (r-vaccinia v...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310571
更新日期:1994-01-01 00:00:00
abstract::We analyzed retrospectively a relative risk of measles attacks in vaccinated vs. unvaccinated students using two independent outbreaks in Japan. The first involved 33/328 (10%) students where 64% students and 30% measles cases had been vaccinated. The second involved 27/241 (11%) students where 81% students and 48% me...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309868
更新日期:1995-01-01 00:00:00
abstract::Neutralizing activity against dengue virus types 1--4 was observed in milk samples from 5 non-immune and 29 dengue immune women. Anti-dengue activity in milk and colostrum was found only in the lipid component. The inhibitory activity is directed against the virus and not cell surfaces. When immunoglobulin types IgM,I...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315587
更新日期:1975-01-01 00:00:00
abstract::Treatment of a mouse macrophage cell line Mk1 with pokeweed mitogen (PWM) either before or during but not after virus inoculation resulted in an enhancement of dengue virus (DV) infection. The infection enhancement was primarily due to an increase in the number of DV-infected cells but not to increased virus productio...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379106
更新日期:1994-01-01 00:00:00
abstract::We investigated the in situ localization of the 50 kDa protein encoded by ORF2 of Apple chlorotic leaf spot virus (ACLSV) genome which is thought to be a movement protein. In immunogold electron microscopy of ACLSV-infected Chenopodium quinoa leaves, the 50 kDa protein was localized on plasmodesmata and nearby cytopla...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050660
更新日期:1999-01-01 00:00:00
abstract::A study was made of the phylogenetic relationships between fifteen complete nucleotide sequences as well as 43 nucleotide sequences of the putative coat protein gene of different strains belonging to the virus species Beak and feather disease virus obtained from 39 individuals of 16 psittacine species. The species inc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0368-x
更新日期:2004-12-01 00:00:00
abstract::The cytomorphologic method previously reported for titration of adenoviruses has been employed for estimating the infectious titer of simian virus 40 (SV 40). Infected cells forming intranuclear inclusions were determined. The method examined possesses a number of advantages over virus titration by plaque assay and cy...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01341731
更新日期:1982-01-01 00:00:00
abstract::The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large qua...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01321065
更新日期:1994-01-01 00:00:00
abstract::A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313815
更新日期:1989-01-01 00:00:00
abstract::Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by the SFTS virus (SFTSV). Hubei Province is a major epidemic area for SFTS in China. In this study, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and serological testing (IgM) were used simultaneously ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3993-5
更新日期:2018-12-01 00:00:00
abstract::Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this populati...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3224-5
更新日期:2017-06-01 00:00:00
abstract::Begomovirus infection is becoming a threat in fresh-market tomato in Argentina, where mixed infections with begomoviruses are common. The complete sequence of a begomovirus isolate infecting tomato sampled in Salta was molecularly characterized. Phylogenetic analysis showed that this virus isolate is closely related t...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1355-2
更新日期:2012-10-01 00:00:00
abstract::Hepatitis B virus (HBV) circulates as a collection of genetically related variants that evolve throughout the chronic infection. Those viral variants that have the greatest fitness are fixed. We recently showed different fitness for HBV variants involved in two epidemiological situations. To understand these fitness d...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-4090-5
更新日期:2019-02-01 00:00:00
abstract::Arginine is necessary for the development of the cytopathogenic effect of human cytomegalovirus in human embryonic fibroblasts. It is also required, though in greater concentrations, for the production of infective virions, the requirement being at an early stage of replication. Inhibitor studies suggested that this b...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318146
更新日期:1975-01-01 00:00:00
abstract::In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718291
更新日期:1996-01-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has evolved several immune-evasion strategies. One strategy involves encoding Fcgamma receptor (FcγR)-like proteins that thwart the Fcγ-mediated effector functions. Our aim was to determine whether GM allotypes-immunoglobulin γ chain determinants expressed primarily on the Fc segment-modul...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-0937-8
更新日期:2011-05-01 00:00:00
abstract::In this study, bovine enteric caliciviruses (BECs) were detected in 49.4% of a total of 253 stool specimens for diarrheic calves collected from 42 industrial dairy farms from March 2010 to February 2012. Genogroup III norovirus (NoVsGIII) were more prevalent (39.5%) than neboviruses (NBs) (15%), and coinfections were ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3716-y
更新日期:2018-05-01 00:00:00