Molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species.

Abstract:

:When 66 cucurbit samples with yellowing symptoms from fields in Mali, the Philippines, Thailand and Uzbekistan were screened by RT-PCR using universal polerovirus primers, 21 were identified as harboring polerovirus RNA. When these 21 samples were screened with specific primers for the known cucurbit-infecting poleroviruses, suakwa aphid-borne yellows virus and a recombinant strain of cucurbit aphid-borne yellows virus were detected for the first time in the Philippines and Thailand. However, seven polerovirus-positive samples did not react with any of the known species-specific primers. Sequencing of 1.4-kb universal polerovirus RT-PCR products revealed the presence of two poleroviruses that had not been described previously. These viruses, from Mali and Thailand, were provisionally named pepo aphid-borne yellows virus and luffa aphid-borne yellows virus, respectively.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Knierim D,Tsai WS,Maiss E,Kenyon L

doi

10.1007/s00705-013-1939-5

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

1459-65

issue

6

eissn

0304-8608

issn

1432-8798

journal_volume

159

pub_type

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