Abstract：
:Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this population. Sixty-four HCV-positive IDUs in Montenegro were enrolled between 2013 and 2014, and the NS5B gene was sequenced. The Bayesian analysis showed that the most prevalent subtype was HCV-3a. Phylogenetic data showed that HCV-3a reached Montenegro in the late 1990s, causing an epidemic that exponentially grew between the 1995 and 2005. In the dated tree, four different entries, from 1990 (clade D), 1994 (clade A) to 1999 (clade B) and 2001 (clade C), were identified. In the NS5B protein model, the amino acids variations were located mainly in the palm domain, which contains most of the conserved structural elements of the active site. This study provides an analysis of the virus transmission pathway and the evolution of HCV genotype 3a among IDUs in Montenegro. These data could represent the basis for further strategies aimed to improve disease management and surveillance program development in high-risk populations.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Mugosa B,Cella E,Lai A,Lo Presti A,Blasi A,Vratnica Z,Vujoševic D,Ebranati E,Lauševic D,Guarino M,Zehender G,Milano T,Pascarella S,Spoto S,Angeletti S,Ciccozzi Mdoi
10.1007/s00705-017-3224-5subject
Has Abstractpub_date
2017-06-01 00:00:00pages
1549-1561issue
6eissn
0304-8608issn
1432-8798pii
10.1007/s00705-017-3224-5journal_volume
162pub_type
杂志文章abstract：:The process of fusion from without (FFWO) induced by herpes simplex virus (HSV) was analyzed by using various inhibitors and compared to fusion from within (FFWI). The fate of certain elements of the cytoskeleton after FFWO was also investigated. Our experiments demonstrate FFWO as a very suitable system for study of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310490
更新日期：1991-01-01 00:00:00
abstract：:Measles virus and mumps virus (MeV and MuV) are enveloped RNA viruses used for production of live attenuated vaccines for prophylaxis of measles and mumps disease, respectively. For biotechnological production of and basic research on these viruses, the preparation of highly purified and infectious viruses is a prereq...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2801-3
更新日期：2016-06-01 00:00:00
abstract：:DNA polymerase (DNApol) is present in all baculoviruses and plays a crucial role in viral DNA replication. Previously we showed that the DNApol of the alphabaculovirus group II Spodoptera litura nucleopolyhedrovirus (SpltNPV) could partially substitute for the DNApol of a group I alphabaculovirus, Autographa californi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3468-0
更新日期：2017-11-01 00:00:00
abstract：:Brefeldin A (BFA) induced a rapid redistribution of vesicular stomatitis virus G protein (VSV-G) to the endoplasmatic reticulum (ER) in interferon (IFN)-pretreated cells. This result is consistent with accumulation of VSV-G in the trans-Golgi (GC) complex in cells pretreated with IFN and implies that IFN does not inte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314635
更新日期：1992-01-01 00:00:00
abstract：:Hemorrhagic fever with renal syndrome (HFRS) is endemic in East Asia and Europe. The disease is caused by several viruses belonging to the genus Hantavirus, including the Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava Belgrade virus (DOBV), and Puumala virus (PUUV). Recently, HTNV-related viruses, Amur (AMR) and Fa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0113-x
更新日期：2003-08-01 00:00:00
abstract：:Work from a number of laboratories including our own has shown that foreign B-epitopes inserted into the c/e1-region of Hepatitis B core antigen (HBcAg) elicit powerful antibody responses when mice are immunised with the recombinant core particles. In the present study, we wished to take advantage of the immunodominan...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050590
更新日期：1999-01-01 00:00:00
abstract：:We have sequenced the genome of a novel picornavirus, rabovirus A (rat-borne virus, RaBoV-A, NC_026314), which was present in the feces of a Norway rat (Rattus norvegicus) from Berlin, Germany. This virus is related to members of the genera Enterovirus and Sapelovirus. RaboV-A contains a type II IRES that is unlike th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2523-y
更新日期：2015-10-01 00:00:00
abstract：:Tetracyclines have been used to treat many bacterial infections. The use of these antibiotics for the treatment of viral diseases dates to the 1960s to 1970s. Over the decades, the effect of tetracyclines on the pathogenesis of viral infections has been demonstrated both clinically and experimentally. Tetracyclines ca...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-020-04860-8
更新日期：2021-01-01 00:00:00
abstract：:The sequence variability of Barley yellow dwarf virus-PAV (PAV) and Barley yellow dwarf virus-MAV (MAV) was studied by comparing 502 nucleotides from the coat protein-encoding region of six isolates from Latvia and four from Sweden. The diversity within MAV was low (>97% sequence identity), also when compared to isola...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0242-2
更新日期：2004-04-01 00:00:00
abstract：:In this study, we isolated a mycobacteriophage infecting Mycobacterium smegmatis mc2155 from a soil sample collected in Shandong Province in China. This phage was named Shandong1. It is a member of the family Siphoviridae with an isometric head and a long tail. Its genome was found to be 60,618 bp long with 67.46% G +...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3534-7
更新日期：2017-12-01 00:00:00
abstract：:Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2473-4
更新日期：2015-08-01 00:00:00
abstract：:Hybridomas secreting monoclonal antibodies against herpes simplex virus (HSV) DNA binding proteins (DBP) have been produced. Five HSV DBP have been characterized according to molecular weight, affinity for DNA, kinetic class and localization within the infected cell. By preparing an immunoadsorbent column from antibod...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314300
更新日期：1984-01-01 00:00:00
abstract：:The receptor for adeno-associated virus serotype 2 (AAV2) in Purkinje cells has not been identified, but based on work carried out in non-neuronal cell lines, heparan sulfate proteoglycan is thought to act as a primary receptor, with basic fibroblast growth factor receptor-1 being reported as a co-receptor. In this st...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0222-7
更新日期：2008-01-01 00:00:00
abstract：:We constructed three sub-genomic replicons of Tick-borne encephalitis virus (TBEV) (Oshima REP, Oshima REP-GFP and Oshima REP-Neo) by deleting genes coding for structural proteins without or with insertion of green fluorescent protein (GFP) or Neo genes, respectively. BHK cells transfected with Oshima REP expressed th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0262-y
更新日期：2004-06-01 00:00:00
abstract：:In attempts to further develop murine leukemia virus (MLV) based retroviral vectors for gene therapy, we investigated vector production and antisense expression from retroviral constructs with U3 deletions or insertions. Promoter elements in the U3 region of the 3' LTR of the vector pLXSN were deleted and replaced wit...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170029
更新日期：2001-01-01 00:00:00
abstract：:Broadly neutralizing antibodies (bNAbs) are very promising agents for HIV-1 prophylaxis and AIDS treatment. However, the neutralization susceptibility of circulating recombinants such as CRF01_AE, which is becoming increasingly prevalent, has not been studied in detail until now. Here, we focused on CRF01_AE in China ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4011-7
更新日期：2018-12-01 00:00:00
abstract：:Reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the rapid laboratory diagnosis of pestivirus infections. A direct DNA sequencing method was developed for the analysis of the amplified cDNA from the 5' noncoding region of the viral genome. 70 pestivirus strains were compared in th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309467
更新日期：1994-01-01 00:00:00
abstract：:Agarose in situ digestion was used to prepare intact Amsacta moorei entomopoxvirus (AmEPV) DNA from embedded occlusion bodies (OBs). Direct dissolution of OBs in agarose eliminated the necessity for separate purification of virions. A physical map of AmEPV DNA was constructed for five restriction enzymes (BamHI, EcoRI...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310704
更新日期：1990-01-01 00:00:00
abstract：:The combined use of phage and antibiotics can show synergistic antimicrobial effects, so-called phage-antibiotic synergy (PAS). Here, we screened and examined PAS against Pseudomonas aeruginosa in vitro. Testing four different phages infecting P. aeruginosa, phage KPP22 classified within the family Myoviridae genus Pb...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3811-0
更新日期：2018-07-01 00:00:00
abstract：:Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, hum...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0396-7
更新日期：2009-01-01 00:00:00
abstract：:The high prevalence (14.3%) of HIV/HBV co-infections reported in west China makes it necessary to screen concurrent HBV infection in HIV carriers. HBV B genotype was shown to be dominant in 54 cases of HIV/HBV co-infection, accounting for 81.48% of the total. The total drug resistance rate observed was 3.70%. A1762T, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3900-0
更新日期：2018-10-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection has a major effect on health care systems, with about one-third of the world's population currently infected with the virus. There is an effective vaccine against HBV, which contains a recombinant "surface antigen" produced in an expression vector. Vaccination has proved to be success...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0206-7
更新日期：2008-01-01 00:00:00
abstract：:A total of 34 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strains isolated from Vietnam during 2013-2014 were sequenced and analyzed. A partial sequence of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and the full sequence of open reading frame 5 (ORF5) g...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2699-1
更新日期：2016-03-01 00:00:00
abstract：:Coxsackievirus B (CVB) can cause aseptic meningitis, myocarditis and respiratory disease, especially in newborn infants. To compare the susceptibility to CVB infection of fetal and adult mice, we prepared primary alveolar epithelial cells (AECs) from lungs of BALB/c mice. In contrast to fetal mouse AECs, those of adul...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1254-6
更新日期：2012-06-01 00:00:00
abstract：:Cellular uptake of fowl plague virus occurs 10-30 minutes after inoculation of chick embryo cells. The penetration of the virions is by pinocytosis (viropexis); fusion with the cellular membrane has not been observed. After pinocytosis the virions become gradually disintegrated. Budding of newly formed virions from th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317537
更新日期：1975-01-01 00:00:00
abstract：:IG 1, a temperate phage of Bacillus subtilis, is strongly induced from its lysogens by 6-(p-hydroxyphenylazo)-uracil, an azopyrimidine known as selectively inhibiting the B. subtilis DNA polymerase III. IG 1 phages originated either by induction or infection multiply, abundantly, in the presence of that azopyrimidine,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316671
更新日期：1990-01-01 00:00:00
abstract：:The DNA of mammalian cells treated with 4,5',8-trimethylpsoralen (Trioxsalen) is crosslinked (preventing transcription) only after photoactivation with near ultraviolet light. Suppression of gene action in cocultivated or fused cultures, including both induction and antiviral action of fibroblast interferon, can conse...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320762
更新日期：1980-01-01 00:00:00
abstract：:Eco RI fragment G of equine herpesvirus 4 strain 405/76 (EHV 4.405/76) is located at the left end of the unique short region close to or extending into the internal repeat region of the prototypic arrangement of the genome. The nucleotide sequence of two subclones designated HS and G 19, contiguous within Eco RI fragm...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309795
更新日期：1993-01-01 00:00:00
abstract：:Noroviruses, a major cause of acute gastroenteritis outbreaks worldwide, are constantly evolving. This ability is reflected in the speed and efficiency with which these viruses spread and remain in the human population. The present study reports the detection of a novel recombination event among norovirus genotypes in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1428-2
更新日期：2012-12-01 00:00:00
abstract：:Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not availa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3806-x
更新日期：2018-07-01 00:00:00
