Abstract:
:Site-directed mutagenesis was used to study the biological significance of three N-linked glycans (linked to Asn406, Asn448, and Asn463), situated in the CD4-binding region of gp120. Mutagenesis was carried out in a phage M13 system, and the mutated env genes were inserted into recombinant vaccinia virus (r-vaccinia virus). To evaluate if the level of expression affected the biological phenotype of mutant gp120, we expressed the envelope glycoproteins using either a weak (7.5 K) or a strong (11 K) promoter of vaccinia virus. The expression of mutated env proteins was analyzed after infecting CD4-expressing HeLa cells with the r-vaccinia virus, by monitoring the ability of the infected cells to generate CD4-dependent syncytia. Env gene products lacking all three glycans as well as env gene products lacking different permutations of one or two glycans were analyzed. All mutated gp120 species had the expected electrophoretical mobility as anticipated from elimination of one, two, and three N-linked glycans, respectively. Moreover, all mutant env gene products demonstrated the same capacity to induce formation of syncytia, irrespective of using the weak or strong promoter for expression. These data indicate that the three N-linked glycans studied are dispensable for HIV env gene products to function in CD4-binding and the subsequent fusion step.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hemming A,Bolmstedt A,Jansson B,Hansen JE,Travis B,Hu SL,Olofsson Sdoi
10.1007/BF01310571subject
Has Abstractpub_date
1994-01-01 00:00:00pages
335-44issue
3-4eissn
0304-8608issn
1432-8798journal_volume
134pub_type
杂志文章abstract::A porcine teschovirus (PTV) was isolated from a dead piglet from a herd of 200 sows showing reproductive failure in Fuyu, Heilongjiang Province, China. Sequencing of most of the genome of this isolate, designated Fuyu/2009, revealed that is was a PTV-8 isolate, closely related to a previously identified Chinese PTV-8 ...
journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
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journal_title:Archives of virology
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journal_title:Archives of virology
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pub_type: 杂志文章
doi:10.1007/BF01341731
更新日期:1982-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:1981-01-01 00:00:00
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journal_title:Archives of virology
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更新日期:1995-01-01 00:00:00
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更新日期:1985-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314237
更新日期:1985-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1552-z
更新日期:2013-07-01 00:00:00
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更新日期:2006-12-01 00:00:00
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journal_title:Archives of virology
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journal_title:Archives of virology
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journal_title:Archives of virology
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更新日期:1985-01-01 00:00:00
abstract::Bovine leukemia virus (BLV) is a member of the genus Deltaretrovirus of the family Retroviridae and cause a chronic lymphosarcoma, which is extensive in cattle. In yaks (Bos grunniens), the distribution, strains and genetic characteristics of BLV have rarely been studied. The aim of our study was to investigate BLV in...
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abstract::Cytomegalovirus (CMV) infections are common after transplantation, but usually successfully treated with antivirals. In this study, the detection of CMV-DNA in peripheral blood leukocytes was monitored and compared with CMVpp65-antigenemia in liver transplant patients receiving ganciclovir treatment. Twenty adult live...
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00
abstract::Rice transitory yellowing virus (RTYV), a member of the genus Nucleorhabdovirus, is closely related to or synonymous with rice yellow stunt virus (RYSV). To clarify the relationship between RTYV and RYSV, we determined the nucleotide sequence of the RTYV genome. The RTYV genome consists of 14,029 nucleotides. The over...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0557-8
更新日期:2010-02-01 00:00:00
abstract::Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information on other continents is lacking. The aim of this study was to describe PeV circulation in a cohort of children in Malawi, Afri...
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pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2018-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00