Abstract:
:Cytomegalovirus (CMV) infections are common after transplantation, but usually successfully treated with antivirals. In this study, the detection of CMV-DNA in peripheral blood leukocytes was monitored and compared with CMVpp65-antigenemia in liver transplant patients receiving ganciclovir treatment. Twenty adult liver transplant recipients were frequently monitored for CMV up to 6 months after transplantation. CMV infections were diagnosed by pp65-antigenemia and the same specimens were used for CMV-DNA in situ hybridization. Altogether 202 blood specimens were analyzed. During the first 6 months, 14/20 patients developed CMV antigenemia and 11 were treated with ganciclovir. In all patients, CMV-DNA was detected before antigenemia (mean 15 days earlier). All patients responded to ganciclovir and pp65-antigenemia disappeared. However, 8/11 demonstrated persistence of CMV-DNA for up to 6 months. Recurrences appeared in 6/11 patients. In conclusion, detection of CMV-DNA preceded pp65-antigenemia. Persistence of CMV-DNA demonstrates that the virus is not eliminated by ganciclovir and recurrences can be expected.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Loginov R,Höckerstedt K,Lautenschlager Idoi
10.1007/s00705-003-0108-7keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
1269-74issue
7eissn
0304-8608issn
1432-8798journal_volume
148pub_type
杂志文章abstract::Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the m...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050675
更新日期:2000-01-01 00:00:00
abstract::Chrysanthemums worldwide suffer from a high incidence of infection with chrysanthemum virus B (CVB), a member of the genus Carlavirus, family Betaflexiviridae. Three major lineages or strains of this virus have been found in India, but none have been characterized beyond the genetic variation they display in their coa...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1190-x
更新日期:2012-03-01 00:00:00
abstract::Since late 2018, foot-and-mouth disease (FMD) has reemerged and rapidly swept through pig farms in North and Central Vietnam, despite widespread use of commercial FMD vaccines. To investigate the FMD virus (FMDV) strains responsible for the current epidemics, 40 FMDV samples were collected from 17 provinces during Nov...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04763-8
更新日期:2020-11-01 00:00:00
abstract::The genome of a Spanish isolate of Broad bean wilt virus-1 (BBWV-1) was completely sequenced and compared with available sequences of other isolates of the genus Fabavirus (BBWV-1 and BBWV-2). This consisted of two RNAs of 5814 and 3431 nucleotides, respectively, and their organization was similar to that of other mem...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0582-1
更新日期:2005-10-01 00:00:00
abstract::Four H7N3 avian influenza viruses (AIVs) were isolated from domestic ducks in live-poultry markets in Zhejiang Province, Eastern China, in 2011. All viruses were characterized by whole-genome sequencing with subsequent phylogenetic analysis and genetic comparison. Phylogenetic analysis of all eight viral genes showed ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1370-3
更新日期:2012-10-01 00:00:00
abstract::The small intestine of piglets orally infected with rotavirus was examined by electron microscopy 18, 24, 48 and 60 hours after infection. At 18 and 24 hours after infection columnar epithelial cells covered the villi. Infected epithelial cells tended to be less electron-dense than uninfected cells and were more numer...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317902
更新日期:1979-01-01 00:00:00
abstract::IG 1, a temperate phage of Bacillus subtilis, is strongly induced from its lysogens by 6-(p-hydroxyphenylazo)-uracil, an azopyrimidine known as selectively inhibiting the B. subtilis DNA polymerase III. IG 1 phages originated either by induction or infection multiply, abundantly, in the presence of that azopyrimidine,...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316671
更新日期:1990-01-01 00:00:00
abstract::Synthesis of murine gamma interferon (MuIFN gamma) by phytohemagglutinin (PHA)-stimulated spleen cells was inhibited in a dose-dependent manner by graded concentrations of tunicamycin or 2-deoxy-glucose, both of which inhibit glycosylation. The homologous (murine) and heterologous (rat) antiviral activities of the MuI...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310659
更新日期:1984-01-01 00:00:00
abstract::A total of 472 samples from domestic pigs collected in China from 2015 to 2018 were tested for the presence of porcine circovirus types 2 and 3 (PCV2 and PCV3, respectively) by conventional polymerase chain reaction analysis. The prevalence of PCV2, PCV3, and PCV2/3 co-infection was 50.0%, 13.3%, and 6.78%, respective...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04336-4
更新日期:2019-10-01 00:00:00
abstract::The in vitro cytopathology and the neurovirulence to inbred mice demonstrated by five variants originally derived from one laboratory strain (Miyama) of type 1 herpes simplex virus (HSV-1) were studied comparatively. Three of the variants are syncytial [+GC (LPV), +GC (SPV), +GC (81)] and two are non-syncytial [-GCr a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317369
更新日期:1986-01-01 00:00:00
abstract::The infectivity of the H9N2 virus to MDCK cells was time-dependently inhibited by Cu(2+) at concentrations of 2.5-250 microM. In 25 microM Cu(2+) solution, the virus titer decreased by approximately 3 and 4 log within 3 and 6 h, respectively. Compared to Cu(2+), Zn(2+) was much less effective in virus inactivation. Th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0154-2
更新日期:2008-01-01 00:00:00
abstract::Norovirus has captured increasing attention as an agent of childhood diarrhoea. However, it is not known whether norovirus causes as severe diarrhoea as rotavirus, particularly among children in developing countries. In a 1-year study conducted between May 2004 and April 2005 in Recife, Brazil, norovirus was detected ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0060-7
更新日期:2008-01-01 00:00:00
abstract::A rotavirus-like virus has been isolated from cases of neonatal diarrhoea in piglets. No antigenic relationship with known rotaviruses or with an American strain of a rotavirus-like virus has been demonstrated. Morphologically the virus is similar to known rotaviruses, but it differs in the ability to form syncytia of...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311220
更新日期:1984-01-01 00:00:00
abstract::A 5-year-old female Yorkshire terrier dog died a few days following hernia and ovariohysterectomy surgeries. Necropsy performed on the dog revealed that the surgeries were not the cause of death; however, degenerative viral hepatitis, showing intranuclear inclusion bodies in hepatic cells, was observed in histopatholo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2895-7
更新日期:2016-08-01 00:00:00
abstract::Complementary oligonucleotide primers which flank a 1146-nucleotide gene fragment (S1B: nt 1185 to 2333) encompassing a polymorphic region (nt 1368 to 1776) of the S1 subunit of bovine coronavirus spike glycoprotein were used for enzymatic amplification by PCR. We chose four clinical isolates, recovered from cases of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310017
更新日期:1994-01-01 00:00:00
abstract::Five cDNA probes prepared from molecular clones representing genomic RNA sequences of the virulent Miller strain of transmissible gastroenteritis virus (TGEV) were used in a dot blot hybridization assay to detect TGEV in cell culture and fecal specimens. Two clones (pA2 and pB4) represent nucleotide base pairs at the ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01319234
更新日期:1991-01-01 00:00:00
abstract::Hepatitis C virus (HCV) genome contains a 3'noncoding region (3'NCR) consisting of a variable region, a polypyrimidine tract (polyU/UC) and the X region. To examine the roles of 3'NCR and polyU/UC tract in the internal ribosome entry site (IRES)-mediated translation process, a variety of 3'NCRs containing different le...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0491-3
更新日期:2005-06-01 00:00:00
abstract::We focused on the role of membrane bound sugar residues in the infection of fibroblasts and monocyte-like cells with human cytomegalovirus (HCMV). Treatment of phorbol 12-myristate 13-acetate (PMA) differentiated monocyte-like cells THP-1 or human fibroblasts MRC-5 with lectins specific for N-acetylneuraminic acid (Ne...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322663
更新日期:1995-01-01 00:00:00
abstract::Thrips palmi transmits the tospoviruses watermelon bud necrosis (WBNV) and groundnut bud necrosis virus (GBNV) in persistent propagative way. Little is known about the T. palmi-WBNV and -GBNV relationship. In this study, we report the effects of WBNV and GBNV infection on the life history traits of T. palmi. Both WBNV...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04381-z
更新日期:2019-11-01 00:00:00
abstract::The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence ide...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2832-9
更新日期:2016-07-01 00:00:00
abstract::Ultraviolet (UV) inactivation and photoreactivation of Anacystis nidulans and cyanophage AS-1 was studied at different wavelengths. UV inactivation of free phage particles and one and two hour host-phage complexes (intracellular phages) were exponential. UV resistance of plaque forming units was attained at the latter...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317413
更新日期:1979-01-01 00:00:00
abstract::In order to improve the knowledge of the humoral immune response to CMV infection, we developed an immunoblotting technique which allowed a better analysis of the changes in the pattern of anti CMV-polypeptides IgM. We examined 234 sera belonging to 27 renal allograft recipients developing a primary or recurrent CMV i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310938
更新日期:1989-01-01 00:00:00
abstract::Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major anti...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050070087
更新日期:2000-01-01 00:00:00
abstract::Sapoviruses (SaVs) belong to the family Caliciviridae and are related to gastroenteritis viruses of humans and animals. These agents have been reported from several countries of the world and represent an important cause of economic loss. The Amazon area has a high degree of diversity of animals and plants, is located...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1904-3
更新日期:2014-05-01 00:00:00
abstract::In this study, an Escherichia coli virulent phage, SRT8, was isolated from sewage sludge samples collected from Jinan, Shandong Province, China. The genome of phage SRT8 consists of 49,579 bp with 47.83% G+C content. The phage genome contains 84 putative protein-coding genes, and no rRNA or tRNA genes. Comparative gen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3778-x
更新日期:2018-06-01 00:00:00
abstract::A nucleotide fragment encoding amino acids 29 to 94 in the E-protein of 28 dengue-2 isolates of diverse geographic and host origins was examined by direct sequencing of a polymerase chain reaction (PCR)-amplified product, and compared to six previously published sequences. Nucleotide divergence ranged from 0 to 19.8% ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316895
更新日期:1993-01-01 00:00:00
abstract::A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck, and goose reovirus (ARV, DRV, and GRV) RNA from cell culture supernatant and clinical samples was established. Based on multiple sequence alignment, a pair of degenerate primers was selected and synthesized. The ampl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0731-1
更新日期:2006-08-01 00:00:00
abstract::We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3363-8
更新日期:2017-08-01 00:00:00
abstract::Genomic information about Muscovy duck parvovirus is still limited. In this study, the genome of the pathogenic MDPV strain YY was sequenced. The full-length genome of YY is 5075 nucleotides (nt) long, 57 nt shorter than that of strain FM. Sequence alignment indicates that the 5' and 3' inverted terminal repeats (ITR)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2946-0
更新日期:2016-09-01 00:00:00
abstract::The aim of this study was to develop and evaluate a two-tube multiplex real-time RT-PCR assay for the detection and identification of four viral hemorrhagic fever (VHF) pathogens, severe fever with thrombocytopenia syndrome virus (SFTSV), Hantaan virus (HTNV), Seoul virus (SEOV), and dengue virus (DENV), from human cl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1677-8
更新日期:2013-09-01 00:00:00