Abstract：
:The VP1 encoded by genomic segment B of birnaviruses is generally known to exist as a genome-linked protein (VPg) and as a "free" polypeptide of 90 kDa in virus particles. The guanylylation activity associated with infectious bursal disease virus (IBDV) was demonstrated by incubating purified virus in presence of [alpha 32P] GTP; optimum activity in the 90 kDa form of VP1 was seen in low salt concentration in the presence of 4 mM magnesium ions over a wide range of incubation temperatures. The IBDV VP1 was shown to lack guanyl transferase activity. Northwestern (RNA-protein) blot analysis of purified virus using a radiolabelled cDNA probe consisting of 3' and 5' ends of genomic segment B indicated that both forms of virion-associated VP1 contained viral RNA sequences of which those linked to VPg corresponded to the two genome segments and those linked to the 90 kDa VP1 were probably a short oligonucleotide of the terminal viral RNA sequences.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Kibenge FS,Dhama Vdoi
10.1007/s007050050154subject
Has Abstractpub_date
1997-01-01 00:00:00pages
1227-36issue
6eissn
0304-8608issn
1432-8798journal_volume
142pub_type
杂志文章abstract：:The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degree...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309574
更新日期：1992-01-01 00:00:00
abstract：:The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variatio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0307-3
更新日期：2009-01-01 00:00:00
abstract：:Genetic analysis of the complete haemagglutinin (HA) gene of fourteen Nigerian avian influenza isolates showed multiple basic amino acids at the cleavage site (321PQRERRRK del R*GLF333), characteristic of highly pathogenic avian influenza (HPAI). Substitution of Gln to Lys at position 322 (H5-specific numbering) was i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3149-4
更新日期：2017-03-01 00:00:00
abstract：:The nature of SRV-4 infection in cynomolgus macaques remains unclear to date. Here, we report the monitoring of 24 cynomolgus monkeys that were naturally infected with SRV-4 for virus isolation, proviral load and antibody. The results indicated that the SRV-4 antibody status was statistically correlated to environment...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1068-y
更新日期：2011-11-01 00:00:00
abstract：:The complete genomes of a monopartite begomovirus (genus Begomovirus, family Geminiviridae) and an associated betasatellite found infecting Vernonia amygdalina Delile (family Compositae) in Uganda were cloned and sequenced. Begomoviruses isolated from two samples showed the highest nucleotide sequence identity (73.1% ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3175-2
更新日期：2017-04-01 00:00:00
abstract：:Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent memb...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050332
更新日期：1998-01-01 00:00:00
abstract：:A new virus belonging to the family Dicistroviridae was identified in the hibiscus-infesting cotton mealybug Phenacoccus solenopsis. Using high-throughput sequencing (HTS) on an Illumina HiSeq platform, a single contig of the complete genome sequence was assembled. The authenticity of the sequence obtained by HTS was ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04702-7
更新日期：2020-09-01 00:00:00
abstract：:Sapoviruses (SaVs) belong to the family Caliciviridae and are related to gastroenteritis viruses of humans and animals. These agents have been reported from several countries of the world and represent an important cause of economic loss. The Amazon area has a high degree of diversity of animals and plants, is located...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1904-3
更新日期：2014-05-01 00:00:00
abstract：:AcDNA library was generated from purified RNA of sugarcane streak mosaic virus--Andhra Pradesh (SCSMV-AP). Two overlapping clones covering 3160 nucleotides encoding partial CI, complete 6K2, VPg-NIa and NIb genes were sequenced. A comparison of this sequence along with the 3' terminal 1315 nucleotides of SCSMV-AP dete...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0851-1
更新日期：2002-10-01 00:00:00
abstract：:The gammaherpesvirus alcelaphine herpesvirus 1 (AlHV-1) causes fatal malignant catarrhal fever (MCF) in susceptible species including cattle, but infects its reservoir host, wildebeest, without causing disease. Pathology in cattle may be influenced by virus-host cell interactions mediated by the virus glycoproteins. C...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2701-y
更新日期：2016-03-01 00:00:00
abstract：:An arracacha (Arracacia xanthorrhiza) plant collected in Brazil was found to be infected by a new virus. This viral isolate (named C17) systemically infected Nicotiana benthamiana and Apium graveolens. A polyclonal antibody was raised, and analysis of our arracacha germplasm collection showed a high infection rate of ...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-008-0289-1
更新日期：2009-01-01 00:00:00
abstract：:A pivotal step in the development of a consistent nomenclature for virus classification was the introduction of the virus species concept by the International Committee on Taxonomy of Viruses (ICTV) in 1991. Yet, almost two decades later, many virologists still are unable to differentiate between virus species and act...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-010-0600-9
更新日期：2010-04-01 00:00:00
abstract：:Molecular epidemiology can be used to identify human immunodeficiency virus (HIV) transmission clusters, usually using pol sequence for analysis. In the present study, we explored appropriate parameters to construct a simple network using HIV env and gag sequences instead of pol sequences for constructing a phylogenet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3485-z
更新日期：2017-11-01 00:00:00
abstract：:A rotavirus-like virus has been isolated from cases of neonatal diarrhoea in piglets. No antigenic relationship with known rotaviruses or with an American strain of a rotavirus-like virus has been demonstrated. Morphologically the virus is similar to known rotaviruses, but it differs in the ability to form syncytia of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311220
更新日期：1984-01-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04842-w
更新日期：2020-12-01 00:00:00
abstract：:The taxonomy of herpesviruses has been updated by the International Committee on Taxonomy of Viruses (ICTV). The former family Herpesviridae has been split into three families, which have been incorporated into the new order Herpesvirales. The revised family Herpesviridae retains the mammal, bird and reptile viruses, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0278-4
更新日期：2009-01-01 00:00:00
abstract：:Colons from ducks and chicken 1, 7, 14 and 28 days old maintained near-normal morphology up to 48 and 96 hours respectively in a system using NCTC135 medium (a part) + Dulbecco's modified Eagle's medium (9 parts ), at 37 degrees C and 95 per cent O2/5 per cent CO2. In the colon of 1 and 28 day-old ducks, duck influenz...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315007
更新日期：1981-01-01 00:00:00
abstract：:Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and qua...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1706-7
更新日期：2013-10-01 00:00:00
abstract：:Chilo iridescent virus (CIV) belongs to the family Iridoviridae, which are icosahedral cytoplasmic DNA viruses with large, linear, and circularly permuted genomes. Previous studies on infected-cell-specific polypeptides suggested temporal regulation of CIV gene expression. Recently, we demonstrated three temporal clas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0232-4
更新日期：2004-04-01 00:00:00
abstract：:A cross-sectional study was undertaken during 2012-2013 to determine the prevalence, strains and factors associated with rotavirus infection among under-5-year-old children hospitalized with acute diarrhea in Uganda. Rotaviruses were detected in 37 % (263/712) of the children. The most prevalent strains were G9P[8] (2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2742-2
更新日期：2016-04-01 00:00:00
abstract：:It has been suggested that antibody overproduction plays a role in the pathogenesis of feline infectious peritonitis (FIP). However, only a few studies on the B-cell activation mechanism after FIP virus (FIPV) infection have been reported. The present study shows that: (1) the ratio of peripheral blood sIg(+) CD21(-) ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0265-9
更新日期：2009-01-01 00:00:00
abstract：:Mengovirus, extracted from infected L-cell cultures with Freon 113 and concentrated from the aqueous phase with polyethyleneglycol, was chromatographed on protein-coated controlled pore glass (CPG). The covalent binding of protein to CPG is described. Further purification and concentration of mengovirus was achieved b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315283
更新日期：1978-01-01 00:00:00
abstract：:Studies on rotavirus non-structural proteins have been hampered in the past by difficulties in obtaining monospecific reagents. To make such reagents available, we have expressed in the baculovirus system NSP2 and NSP3 (formerly called NS35 and NS34, respectively) of the bovine rotavirus RF and produced hybridomas aga...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309746
更新日期：1993-01-01 00:00:00
abstract：:In this study, an improved polymerase chain reaction (PCR) was used for detection of DNA of latent EHV-1 strains from several sources. Three pairs of oligonucleotide primers spanning fragments of 333 bp, 226 bp and 268 bp of the thymidine kinase (tk) gene, and one primer pair spanning 225 bp of the glycoprotein C (gC)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070055
更新日期：2000-01-01 00:00:00
abstract：:The nucleotide sequence of the coat protein cistron of an Austrian isolate of grapevine fanleaf virus (GFLV-FC) from Vitis vinifera cv. French Colombard was determined. It shows small differences at the RNA level as well as at the protein level compared to the sequences of already published grapevine fanleaf virus str...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309730
更新日期：1995-01-01 00:00:00
abstract：:Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not availa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3806-x
更新日期：2018-07-01 00:00:00
abstract：:The adsorption and retention properties of native (unfixed) and glutaraldehyde-fixed alfalfa mosaic virus (AMV) antigens to the polystyrene of ELISA plates were studied using [35S]-labelled virus preparations. It was shown that adsorption was a temperature-dependent, relatively slow process which varied between differ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310767
更新日期：1991-01-01 00:00:00
abstract：:The nucleotide sequences of the SH gene of 45 new mumps virus isolates derived from different parts of Europe, Canada and USA were determined. A phylogenetic tree was constructed which confirmed the existence of three major clusters. While clustering according to geographical origin was clear in some cases each of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050073
更新日期：1997-01-01 00:00:00
abstract：:We had previously showed that following intracerebral inoculation of newborn mice, the 910 N and M strains of bovine parainfluenza 3 virus induce a non-lethal hydrocephalus and a lethal disease with marked thymic and splenic atrophy, respectively. Moreover, only the M virus was lethal for 2-week-old mice. In the prese...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310963
更新日期：1985-01-01 00:00:00
abstract：:Adenovirus type 7 vaccine strain was engineered to express foreign antigens from both the E3 early promoter in the E3 region and the major late promoter inserted between the E4 region and the right inverted terminal repeat. This multiple expression vector was used to express hepatitis B core antigen (HBcAg), hepatitis...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311300
更新日期：1991-01-01 00:00:00
