Abstract：
:Studies on rotavirus non-structural proteins have been hampered in the past by difficulties in obtaining monospecific reagents. To make such reagents available, we have expressed in the baculovirus system NSP2 and NSP3 (formerly called NS35 and NS34, respectively) of the bovine rotavirus RF and produced hybridomas against these proteins. Full-length DNA copies of RNA segments 7 (coding for NSP3) and 8 (coding for NSP2) of the virus strain RF were cloned and sequenced. Each cDNA was inserted in the transfer vector pVL941 and used to transfect Spodoptera frugiperda cells (Sf9). Recombinant baculoviruses encoding these proteins were obtained. Infection of Sf9 cells with these recombinant viruses resulted in a high level of expression of NSP2 and NSP3 (range of 1 microgram per 10(6) cells). Monoclonal antibodies (MAbs) were elicited by immunization of BALB/c mice with adjuvented, unpurified recombinant proteins in the rear foot pads. Fusion was performed using lymphocytes from popliteal lymph nodes with SP2/O-Ag14 myeloma line. Screening was by differential indirect immunofluorescent staining on monolayers of Sf9 cells infected with each recombinant virus. Two MAbs proved to be reactive against NSP3 and a single one against NSP2. They showed high specificity by immunofluorescence, immunoprecipitation and Western blot. The isotype of these MAbs was IgG1. Oligomeric forms of NSP3 and NSP2 proteins were detected and the existence of intra-chain disulfide bridge in NSP2 protein was suggested. The levels of synthesis and cellular localization of NSP3 and NSP2 proteins were different as shown by immunoprecipitation and immunofluorescence.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Aponte C,Mattion NM,Estes MK,Charpilienne A,Cohen Jdoi
10.1007/BF01309746subject
Has Abstractpub_date
1993-01-01 00:00:00pages
85-95issue
1-2eissn
0304-8608issn
1432-8798journal_volume
133pub_type
杂志文章abstract：:The 3'-terminal nucleotides of the genome segments of influenza C/Taylor/47. C/Bavaria/79 and C/Johannesburg/1/66 were identified by two RNA sequencing techniques. These comprised 11 nucleotides (3' UCGUCUUCGUC) which were found to be conserved among the genome segments of each virus. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310664
更新日期：1984-01-01 00:00:00
abstract：:Equine herpesvirus 1 glycoprotein D (EHV-1 gD) expressed constitutively in mammalian cell lines had similar electrophoretic mobility to gD produced in EHV-1 infected cells but lacked a possibly complexed higher molecular weight form seen in the latter. Recombinant gD was N-terminally cleaved at the same site as gD in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718301
更新日期：1996-01-01 00:00:00
abstract：:Coronavirus disease 2019, also known as COVID-19, is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus 2, or SARS-CoV-2. The infection has now catapulted into a full-blown pandemic across the world, which has affected more than 2 million people and has led to approximately 150,000 fatal...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-020-04768-3
更新日期：2020-11-01 00:00:00
abstract：:A high per cent of false positive results for rubella virus IgM antibodies determined by immune fluorescence were detected in sera of pregnant women. After absorption with Staphylococcus suspension, Cowan 1 strain, and aggregates of human immunoglobulins, the false positive reactions due to IgM antiimmunoglobulin were...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01348028
更新日期：1979-01-01 00:00:00
abstract：:The stools of slaughtered pigs were screened for hepatitis E virus (HEV). HEV RNA was detected in 7.3% of the samples. HEV strains were characterized as genotype 3 subtype c, a cluster previously not described in Italy. These findings provide evidence that slaughterhouse workers may be exposed to HEV infection. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0544-0
更新日期：2010-01-01 00:00:00
abstract：:Cucumber mosaic virus (CMV) is a three component isodiametric plant virus which is common worldwide and has an extremely wide host range. A pseudorecombinant was made, derived from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA1,2 components of the CMV-D strain. This system de...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0190-x
更新日期：2004-01-01 00:00:00
abstract：:Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goiás, Maranhão, Mato Grosso, Minas Gerais, Pará) in 2001 and 2010. A variety of symptoms was expressed in soybean cv. CD206, ran...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1879-0
更新日期：2014-04-01 00:00:00
abstract：:A porcine teschovirus (PTV) was isolated from a dead piglet from a herd of 200 sows showing reproductive failure in Fuyu, Heilongjiang Province, China. Sequencing of most of the genome of this isolate, designated Fuyu/2009, revealed that is was a PTV-8 isolate, closely related to a previously identified Chinese PTV-8 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1315-x
更新日期：2012-07-01 00:00:00
abstract：:Since serological donor-screening tests for HIV were introduced in 1985, the safety of donated blood components has improved dramatically. However, these tests do not completely prevent the risk of transfusion-associated HIV infection related to the use of blood donated during the pre-seroconversion window period. Tes...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1085-x
更新日期：2011-11-01 00:00:00
abstract：:The duration of infection with rat virus (RV), an autonomous rodent parvovirus, was examined at multiple intervals over 6 months in rats inoculated by the oronasal route at 2 days of age or 4 weeks of age and individually housed after weaning to prevent cross-infection. Infectious virus was recovered by explant cultur...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310765
更新日期：1991-01-01 00:00:00
abstract：:An attempt has been made to devise computer software that will aid virologists to identify unknown virus isolates using the World Wide Web. Computerized information from the Animal Virus Information System was used to obtain data on various characters of a virus species. Sequence data banks are used to obtain the mole...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050394
更新日期：1998-01-01 00:00:00
abstract：:Rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (MAb) specific for the two envelope glycoproteins of Puumala virus (G1 and G2). The anti-ids were purified by sequential immunoaffinity chromatography. Each anti-id inhibited the antigen binding of its ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309537
更新日期：1993-01-01 00:00:00
abstract：:We investigated the cis-acting sequences of the promoter regulating the gene encoding the glycoprotein C (gC) of bovine herpesvirus 1 (BoHV-1), a gene of the gamma1 class. S1 nuclease protection assays revealed that gC transcription initiated predominantly at position C18281 of the viral genome, corresponding to 21 an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0898-z
更新日期：2003-01-01 00:00:00
abstract：:Strawberry crinkle disease is one of the major diseases that threatens strawberry production. Although the biological properties of the agent, strawberry crinkle virus (SCV), have been thoroughly investigated, its complete genome sequence has never been published. Existing RT-PCR-based detection relies on a partial se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3860-4
更新日期：2018-09-01 00:00:00
abstract：:The use of the complete DNA sequence for the Marek's disease virus (MDV) serotype 1 vaccine strain CVI988 Rispens in comparative genomic studies with virulent strains of MDV has revealed the presence of a number of insertions, deletions and single-nucleotide polymorphisms. In this study, we investigated a SNP in the H...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0152-4
更新日期：2008-01-01 00:00:00
abstract：:A novel virus we call zucchini green mottle mosaic virus (ZGMMV) was isolated from zucchini squash and its properties were determined. The size and shape of its virions, and other properties suggest that the virus is a tobamovirus. The coat protein (CP) genes of ZGMMV and kyuri green mottle mosaic virus (KGMMV), which...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070023
更新日期：2000-01-01 00:00:00
abstract：:Virological surveillance is an important element in the Polio Eradication Initiative to provide information rapidly about circulating wild polioviruses. Molecular tools have been developed to identify the serotype of the poliovirus strains and whether they are of vaccine or wild origin (intratypic differentiation) and...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0001-x
更新日期：2008-01-01 00:00:00
abstract：:A total of 472 samples from domestic pigs collected in China from 2015 to 2018 were tested for the presence of porcine circovirus types 2 and 3 (PCV2 and PCV3, respectively) by conventional polymerase chain reaction analysis. The prevalence of PCV2, PCV3, and PCV2/3 co-infection was 50.0%, 13.3%, and 6.78%, respective...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04336-4
更新日期：2019-10-01 00:00:00
abstract：:Group C rotavirus (GCRV) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. Little is known about the yearly changes in the GCRV strains from diarrheal outbreaks. Stool samples collected from outbreaks of acute gastroenteritis in Yokohama, Japan, betwe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1894-1
更新日期：2014-04-01 00:00:00
abstract：:Although winter dysentery (WD), which is caused by the bovine coronavirus (BCoV) is characterized by the sudden onset of diarrhea in many adult cattle in a herd, the pathogenesis of the WD-BCoV is not completely understood. In this study, colostrum-deprived calves were experimentally infected with a Korean WD-BCoV str...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1005-2
更新日期：2007-01-01 00:00:00
abstract：:The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degree...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309574
更新日期：1992-01-01 00:00:00
abstract：:Enteric caliciviruses, including noroviruses (NoVs) and sapoviruses (SaVs), are recognized as important etiologic agents of acute gastroenteritis (AGE) with considerable genetic diversity. In order to gain an overview of the molecular epidemiology of human NoVs and SaVs in children hospitalized with AGE in Chiang Mai,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3501-3
更新日期：2017-11-01 00:00:00
abstract：:Simian adenovirus SA7 heated for 30 minutes at 70 degrees C retained part of its ability to induce tumors in newborn hamsters. Tumors were also induced by DNA extracted from the heated virus. However, neither residual replicating, i.e. cytopathogenic, nor transforming and T-antigen-inducing activities could be detecte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317334
更新日期：1981-01-01 00:00:00
abstract：:A viroid was isolated from symptomless Coleus blumei cultivar (cv.) 'Rainbow Gold' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viriod was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing reveale...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718222
更新日期：1996-01-01 00:00:00
abstract：:A recombinant encephalomyocarditis virus (rEMCV2887A-egfp) expressing the enhanced green fluorescent protein (EGFP) was produced. The EGFP gene was inserted in frame within the leader protein coding sequence of a full-length cDNA clone of EMCV. RNA transcripts derived from the recombinant full-length cDNA were synthes...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0746-7
更新日期：2006-09-01 00:00:00
abstract：:Persistent infections with high-risk human papillomaviruses (HR-HPVs) are linked to the development of cervical cancer due to a deregulation of the productive viral cycle in the host cell, leading to cell transformation. The E2 viral protein is expressed early during an HPV infection and regulates viral replication an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04124-6
更新日期：2019-07-01 00:00:00
abstract：:Resistance to antiretroviral agents is a significant concern in the clinical management of HIV-infected individuals, particularly in areas of the world where treatment options are limited. In this study, we aimed to identify HIV drug-resistance-associated mutations in 40 drug-naïve patients and 62 patients under antir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2955-z
更新日期：2016-09-01 00:00:00
abstract：:Usurpation of the host's signalling pathways is a common strategy employed by viruses to promote their successful replication. Here we show that infection with the orthopoxvirus vaccinia virus (VACV) leads to sustained stimulation of c-Jun activity during the entire infective cycle. This stimulation is temporally regu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3446-6
更新日期：2017-10-01 00:00:00
abstract：:Virus isolate Y3V, obtained from tobacco showing leaf curl symptoms in Yunnan, China, had particles with the size and morphology typical of geminiviruses. In reactions with a set of monoclonal antibodies raised against begomoviruses, Y3V was readily differentiated from two previously studied Chinese Begomovirus isolat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170081
更新日期：2001-08-01 00:00:00
abstract：:A simple radial method based on the formerly described paper disc technique was developed for demonstration of neutralizing antibodies to enteroviruses with plaque forming ability. Sera administered by 5 microliter wire-loops to wells cut into the agar overlay of virus infected cell monolayers produced radial neutrali...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317320
更新日期：1980-01-01 00:00:00
