Abstract：
:Cucumber mosaic virus (CMV) is a three component isodiametric plant virus which is common worldwide and has an extremely wide host range. A pseudorecombinant was made, derived from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA1,2 components of the CMV-D strain. This system developed mild mosaic and vein clearing in Xanthi tobacco three weeks after inoculation. The CP gene was then engineered in three different positions, to encode a Hepatitis C virus (HCV) epitope. The selected peptide was the so-called R9 mimotope, a synthetic surrogate derived from a consensus profile of many hypervariable region 1 (HVR1) sequences of the putative HCV envelope protein E2. Serum samples from 60 patients with chronic hepatitis C displayed a significant immunoreactivity to crude plant extracts infected with the chimeric CMV. These results suggest that further investigation should be made into a possible vaccine function for the CMV-HCV mimotope system.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Natilla A,Piazzolla G,Nuzzaci M,Saldarelli P,Tortorella C,Antonaci S,Piazzolla Pdoi
10.1007/s00705-003-0190-xkeywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
137-54issue
1eissn
0304-8608issn
1432-8798journal_volume
149pub_type
杂志文章abstract：:We evaluated the importance of dendritic cells (DCs) in the induction of the immune response after immunization of mice with DNA plasmid Auxo-GTU(®)-MultiHIV. First, GTU(®)-encoded protein was shown to be expressed by DCs of the draining lymph nodes (LNs) following intradermal (i.d.) immunization. Next, donor mice wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1003-2
更新日期：2011-09-01 00:00:00
abstract：:Four geographic variants (topotypes) of yellow fever (YF) virus from Africa and South America previously determined by RNA oligonucleotide mapping were analyzed for their structural, antigenic and virulence characteristics. The electrophoretic migration mobility and carbohydrate content of the envelope protein E chara...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310727
更新日期：1987-01-01 00:00:00
abstract：:Cucumber green mottle mosaic virus (CGMMV) is an important pathogen of cucumber (Cucumis sativus). The molecular mechanisms mediating host-pathogen interactions are likely to be strongly influenced by microRNAs (miRNAs), which are known to regulate gene expression during the disease cycle. This study focused on 14 miR...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04152-w
更新日期：2019-04-01 00:00:00
abstract：:Five cDNA probes prepared from molecular clones representing genomic RNA sequences of the virulent Miller strain of transmissible gastroenteritis virus (TGEV) were used in a dot blot hybridization assay to detect TGEV in cell culture and fecal specimens. Two clones (pA2 and pB4) represent nucleotide base pairs at the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01319234
更新日期：1991-01-01 00:00:00
abstract：:Seven different epitopes on arabis mosaic virus (ArMV) were discerned. Neo-, crypto-, and epitopes exposed on the virion and isolated coat protein were differentiated by their reactivity with monoclonal antibodies in an indirect enzyme-linked immunosorbent assay. The monoclonal antibodies, produced in vitro and in viv...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316736
更新日期：1986-01-01 00:00:00
abstract：:Oral inoculation of entomopoxvirus spindles, microstructures composed of fusolin protein, causes disruption of the peritrophic matrix (PM), a physical barrier against microbe infection, in the insect midgut. Although the atomic structure of fusolin has been determined, little has been directly elucidated of the mechan...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4013-5
更新日期：2019-01-01 00:00:00
abstract：:Morphological studies of EIAV reveal knobs on the surface of the particles, conically and tubularly shaped cores, budding particles with dense crescents directly underlying the plasma membrane, and distinct intracytoplasmic structures in infected cells. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315055
更新日期：1977-01-01 00:00:00
abstract：:The genomic RNA of infectious flacherie virus (IFV), an insect picornavirus, was shown to contain a covalently attached substance which can be labeled with 125I-Bolton and Hunter reagent. This substance was found to be a protein covalently linked to the virus genome, because it cosedimented and was copurified with the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317378
更新日期：1986-01-01 00:00:00
abstract：:Betanodaviruses, members of the family Nodaviridae, have small positive-stranded bipartite RNA genomes and are the causal agent of viral nervous necrosis (VNN) in many species of marine farmed fish. In the aquaculture industry, outbreaks of betanodavirus infection and spread in larval and juvenile fish result in devas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1061-7
更新日期：2007-01-01 00:00:00
abstract：:An archival brain sample from an infected dog taken during the 1988/89 rabies outbreak in Finland was investigated to confirm that it was infected with classical rabies virus (genotype 1). The first 400 base pairs of the virus nucleoprotein gene were then sequenced and used to undertake a phylogenetic comparison betwe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0523-z
更新日期：2005-07-01 00:00:00
abstract：:The nucleotide sequence of the granulin gene of Choristoneura fumiferana granulovirus (CfGV) was determined. The gene encodes a protein of 248 amino acids with a predicted Mr of 29.299 kDa. The granulin genes of Trichoplusia ni, Pieris brassicae and Cryptophlebia leucotreta granuloviruses showed homologies ranging fro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050181
更新日期：1997-01-01 00:00:00
abstract：:RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA speci...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1166-x
更新日期：2012-02-01 00:00:00
abstract：:In a virus-infected plant, small interfering RNAs (siRNAs) corresponding to the viral genome form a large proportion of the small RNA population. It is possible to reassemble significant portions of the virus sequence from overlapping siRNA sequences and use these to identify the virus. We tested this technique with a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0979-y
更新日期：2011-07-01 00:00:00
abstract：:Sera and bronchial washings from normal mice and from mice previously infected with influenza virus were analyzed for their concentration of four immunoglobulin (Ig) classes by the method of single radial immunodiffusion and for their content of specific antibody of these Ig classes by the immunofluorescent method. Ig...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314846
更新日期：1977-01-01 00:00:00
abstract：:Ljungan virus is a recently identified member of the family Picornaviridae that was isolated from bank voles in Sweden. LjV has been associated with [corrected] type 1 diabetes-like symptoms and myocarditis in bank voles (Myodes glareolus), and it has been suggested that it has zoonotic potential. Here, we show for th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1731-6
更新日期：2014-03-01 00:00:00
abstract：:The presence of transmitted HIV drug resistance (TDR) threatens the efficacy of antiretroviral treatment. We aimed to assess the changes in TDR prevalence over the last decade in Madrid, Spain, to verify the role of the patients' risk groups in these changes. We analysed the trends of TDR between 2000 and 2011 in a co...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1933-y
更新日期：2014-05-01 00:00:00
abstract：:The gene coding for the nucleocapsid protein NP of the influenza A virus recombinant strain 413 1,1 was characterized biochemically by molecular hybridization and fingerprint analysis. The data presented suggest that this NP gene has evolved by intracistronic recombination between NP genes of virus N and the fowl plag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322701
更新日期：1980-01-01 00:00:00
abstract：:The bisegmented genome of a putative double-stranded (ds) RNA virus from Ustilaginoidea virens was sequenced and analyzed. The larger genomic segment of 2112 bp encodes a putative RNA-dependent RNA polymerase (RdRp, 628 aa), and the smaller one of 2082 bp encodes a putative coat protein (CP) of 539 aa. The 5' untransl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-1991-9
更新日期：2014-07-01 00:00:00
abstract：:A pathogen of significance in the aquaculture sector, the Gram-negative marine bacterium Vibrio parahaemolyticus causes gastroenteritis associated with consumption of improperly prepared seafood. This bacterium can be controlled using lytic bacteriophages as an alternative to antibiotics. ϕVP-1 is a lytic phage of V. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04493-6
更新日期：2020-02-01 00:00:00
abstract：:Drug resistance mutations in the Pol gene of human immunodeficiency virus 1 (HIV-1) are one of the critical factors associated with antiretroviral therapy (ART) failure in HIV-1 patients. The issue of resistance to reverse transcriptase inhibitors (RTIs) in HIV infection has not been adequately addressed in the Indian...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2760-8
更新日期：2016-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0838-y
更新日期：2002-08-01 00:00:00
abstract：:The acid-extractable leaf proteins of potato spindle tuber viroid (PSTV) infected tomato plants were analysed electrophoretically on polyacrylamide gels. The most prominent alteration found during disease development was the appearance of a "pathogenesis-related" protein with an apparent molecular weight of 14,000 (ca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309998
更新日期：1984-01-01 00:00:00
abstract：:Bacteriophage JBP901, isolated from fermented food, is specific for Bacillus cereus group species and exhibits a broad host spectrum among a large number of B. cereus isolates. Genome sequence analysis revealed a linear 159,492-bp genome with overall G+C content of 39.7 mol%, and 201 ORFs. The presence of a putative m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2485-0
更新日期：2015-09-01 00:00:00
abstract：:The prevalence of HIV-1 infection in men who have sex with men (MSM) in China has drastically increased, and circulating strains may have acquired transmitted drug resistance (TDR). We determined TDR genotypes among antiretroviral therapy (ART)-naïve MSM in 19 provinces/cities where HIV-1 prevalence among MSM is high,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1557-7
更新日期：2013-04-01 00:00:00
abstract：:Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310491
更新日期：1991-01-01 00:00:00
abstract：:The revised International Code of Virus Classification and Nomenclature [7] followed by the Seventh Report of the International Committee on Taxonomy of Viruses (ICTV) [9] have generated a lot of criticism [2,4-6]. The main causes of criticism are (i) use of monomials instead of non-latinized binomials, as has been pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200051
更新日期：2002-11-01 00:00:00
abstract：:Integration of bovine leukaemia virus DNA was investigated at all stages of infection in cattle. We report here the detection of integrated proviral DNA in the majority of antibody positive animals without lymphocytosis. In all but one case virus was integrated at a number of different sites. Hybridization analysis fa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314319
更新日期：1991-01-01 00:00:00
abstract：:Several species in the genus Datura (family Solanaceae) are hosts for begomoviruses (family Geminiviridae), both in the New World and the Old World. Here, we report the genome characterization of two isolates of a novel Old World monopartite begomovirus infecting Datura innoxia in Sudan, which we propose to be named "...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3574-z
更新日期：2018-01-01 00:00:00
abstract：:The complete nucleotide sequence (4838 nucleotides) of Iris yellow spot virus (IYSV) M RNA indicates, typical for tospoviruses, the presence of two genes in ambisense arrangement. The vRNA ORF codes for the potential cell-to-cell movement (NSm) protein (34.8 kDa) and the vcRNA ORF for the viral glycoprotein (G1/G2) pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0885-4
更新日期：2002-12-01 00:00:00
abstract：:A sequence-independent single-primer amplification method and a modified enterovirus VP1 gene typing primer were used for identification of echovirus 19 and enterovirus 101, which remained undiagnosed by standard enterovirus molecular typing methods. Six different serotypes were identified during this study, with the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2714-6
更新日期：2016-04-01 00:00:00
