Abstract：
:Betanodaviruses, members of the family Nodaviridae, have small positive-stranded bipartite RNA genomes and are the causal agent of viral nervous necrosis (VNN) in many species of marine farmed fish. In the aquaculture industry, outbreaks of betanodavirus infection and spread in larval and juvenile fish result in devastating damage and heavy economic loss. Although an urgent need exists to develop drugs that inhibit betanodavirus infection, there have been no reports about anti-betanodavirus drugs. Recently, it was reported that betanodaviruses were detected in the endosomes of infected cells, suggesting that betanodaviruses enter fish cells by endocytosis. This finding prompted us to examine whether blocking this endosomal pathway could provide a target for antiviral drug development. In this study, we examined the inhibitory effect of several lysosomotropic agents against betanodavirus infection in fish E-11 cells. The presence of 1 mM NH4Cl or 1 microM chloroquine in the medium inhibited the entry of betanodaviruses into cells and inhibited viral infection. The lysosomotropic agents bafilomycin A1 and monensin also inhibited virus-induced cytopathology and virus production. Our data demonstrate that inhibitors of endosomal acidification are candidates as antiviral agents against betanodavirus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Adachi K,Ichinose T,Takizawa N,Watanabe K,Kitazato K,Kobayashi Ndoi
10.1007/s00705-007-1061-7subject
Has Abstractpub_date
2007-01-01 00:00:00pages
2217-24issue
12eissn
0304-8608issn
1432-8798journal_volume
152pub_type
杂志文章abstract：:Hippeastrum chlorotic ringspot virus (HCRV) is a novel tospovirus that was identified in Yunnan Province, China, in 2013. We have sequenced the HCRV L gene, which is 8909 nt long and encodes the RNA-dependent RNA polymerase (2873 amino acids, 330.8 kDa). The HCRV L protein shared highest similarity (89.4 %) with that ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2100-9
更新日期：2014-10-01 00:00:00
abstract：:Trigeminal ganglia from swine latently infected with pseudorabies virus (PRV) were examined for the presence of PRV nucleic acids by in situ hybridization. Both viral DNA and RNA were detectable in latently infected neurons, indicating that the PRV genome is transcriptionally active during the latent phase of infectio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321010
更新日期：1988-01-01 00:00:00
abstract：:Out of 136 new phages, 80 (59%) are classified into 23 species according to morphology and physicochemical properties. Six new species are described and species beta 4, from a previous classification scheme, is renamed T1. The morphology of 36 phage species is schematically represented. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Porcine circovirus type 2 (PCV2) is the essential infectious agent causing porcine circovirus-associated disease (PCVD) in pigs and one of the important viruses that severely jeopardize the swine husbandry industry. PCV2 elicits the unfolded protein response (UPR) via activation of the PERK pathway, and its capsid pro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04185-1
更新日期：2019-05-01 00:00:00
abstract：:The value of serologic tests for diagnosis of swine influenza virus (SIV) infection has been diminished by the emergence of new subtypes and by antigenic drift within subtype. The intensive use of vaccination also has complicated interpretation of serology results. Serologic assays are needed that can detect infection...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0615-9
更新日期：2006-01-01 00:00:00
abstract：:One host polypeptide (40,000 daltons) synthesized prior to infection is associated with the 250S RNA polymerase structure partially purified by a combination of velocity sedimentation and isopycnic separation. A series of pulse-chase experiments have shown that a 56,000 dalton polypeptide made during the eclipse phase...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317808
更新日期：1975-01-01 00:00:00
abstract：:Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317927
更新日期：1989-01-01 00:00:00
abstract：:We focused on the role of membrane bound sugar residues in the infection of fibroblasts and monocyte-like cells with human cytomegalovirus (HCMV). Treatment of phorbol 12-myristate 13-acetate (PMA) differentiated monocyte-like cells THP-1 or human fibroblasts MRC-5 with lectins specific for N-acetylneuraminic acid (Ne...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322663
更新日期：1995-01-01 00:00:00
abstract：:Human adenovirus Ad36 is causally and correlatively associated in animals and humans, respectively, with increased adiposity and altered metabolic profile. We inoculated rats with Ad36 or UV-inactivated Ad36, or mock-infected them. Four days later, Ad36-infected rats showed 23% greater epididymal fat pad weight and vi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0219-2
更新日期：2008-01-01 00:00:00
abstract：:In vivo transfection by intramuscular injection with plasmids expressing the immunogenic proteins of microbial pathogens has considerable potential as a vaccination strategy against many pathogens of both man and animals. Here we report that weanling mice given a single intramuscular injection of 50 micrograms of a pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718332
更新日期：1996-01-01 00:00:00
abstract：:Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
abstract：:Crucian carp Carassius auratus herpesvirus (CaHV) was isolated from diseased crucian carp with acute gill hemorrhages and high mortality. The CaHV genome was sequenced and analyzed. The data showed that it consists of 275,348 bp and contains 150 predicted ORFs. The architecture of the CaHV genome differs from those of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3037-y
更新日期：2016-12-01 00:00:00
abstract：:The genus "Tupanvirus" is a new proposed taxon to be included in the family Mimiviridae. The two known tupanvirus isolates were isolated from soda lake and oceanic sediments samples collected in Brazil and were named "tupanvirus soda lake" and "tupanvirus deep ocean", respectively. These viruses exhibit similarities t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4067-4
更新日期：2019-01-01 00:00:00
abstract：:The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5' and 3' untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins enco...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3147-6
更新日期：2017-03-01 00:00:00
abstract：:Human cytomegalovirus disappeared from cultures continuously exposed to interferon. When interferon was removed, high titers of virus reappeared. Cytomegalovirus was thus able to persist in potentially infectious form in cells protected by interferon. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309276
更新日期：1983-01-01 00:00:00
abstract：:The present study confirms that herpesviruses (LF) previously isolated from testes and buffy coat cells of male turkeys with semen abnormalities establish a latent infection in testicular cells. These experiments also present the first evidence that these herpesviruses are harbored latently in cells of spinal ganglia....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311163
更新日期：1984-01-01 00:00:00
abstract：:The association of Merkel cell polyomavirus (MCPyV) with Merkel cell carcinoma (MCC) in immunocompromised individuals has been revealed in a number of surveys. The study of MCPyV specific antibody titers and viral loads in such patients has a great attraction for research groups interested in viral reactivation. In th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3186-z
更新日期：2017-04-01 00:00:00
abstract：:Cell cultures inoculated with caprine respiratory syncytial virus (RSV) were studied with light, fluorescent, and electron microscopy to determine the morphogenesis and structure of the virus. Small syncytia were seen after 36 hours in culture. After 48 hours in culture, syncytia were large and numerous and pleomorphi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314543
更新日期：1980-01-01 00:00:00
abstract：:HIV-1 genetic diversity information from a pediatric population is scarce. This study enrolled 128 children living with HIV/AIDS, 103 antiretroviral-treated and 25 naive, from the Sao Paulo metropolitan area. Gag, pol and env regions were amplified, and drug resistance mutations, V3 loop, tropism and viral clades were...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0178-7
更新日期：2008-01-01 00:00:00
abstract：:Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3070-x
更新日期：2016-12-01 00:00:00
abstract：:The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied. Inoculation of either JE or DEN-2 virions into C6/36 cells resulted in direct penetration of the virions into the cytoplasm at the cell su...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313814
更新日期：1989-01-01 00:00:00
abstract：:H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension cu...
journal_title：Archives of virology
pub_type： 临床试验,杂志文章
doi：10.1007/s00705-017-3460-8
更新日期：2017-10-01 00:00:00
abstract：:Viruses are ubiquitous in the biosphere and greatly affect the hosts they infect. It is generally accepted that members of every microbial taxon are susceptible to at least one virus, and a plethora of bacterial viruses are known. In contrast, knowledge of the archaeal virosphere is still limited. Here, a novel lytic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04120-w
更新日期：2019-03-01 00:00:00
abstract：:The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Maclura...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3741-x
更新日期：2018-05-01 00:00:00
abstract：:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314377
更新日期：1977-01-01 00:00:00
abstract：:Bokeloh bat lyssavirus (BBLV) was found in Myotis nattereri for the first time in northeastern France in July 2012. The complete genome sequence of the virus from the infected Natterer's bat was determined by whole-genome sequencing and compared to that of the first BBLV strain isolated in 2010 in Germany and with tho...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1747-y
更新日期：2013-11-01 00:00:00
abstract：:Triple gene block 1 (TGB1) and coat protein (CP) sequences of 30 hosta virus X (HVX) isolates from Tennessee (TN), USA, were determined and compared with available sequences in GenBank. The CPs of all known HVX isolates, including those from TN, shared 98.3-100% and 98.2-100% nucleotide and amino acid sequence identit...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0522-6
更新日期：2009-01-01 00:00:00
abstract：:The taxonomy of herpesviruses has been updated by the International Committee on Taxonomy of Viruses (ICTV). The former family Herpesviridae has been split into three families, which have been incorporated into the new order Herpesvirales. The revised family Herpesviridae retains the mammal, bird and reptile viruses, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0278-4
更新日期：2009-01-01 00:00:00
abstract：:Genetic relationships between the genome segments of six cypovirus (CPV) isolates were analysed by RNA cross-hybridisation. These included three type 1 viruses and single isolates of types 2, 5 and 12, which collectively are identical to those previously compared by serology and electrophoresis [Mertens et al. (1989),...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050525
更新日期：1999-01-01 00:00:00
abstract：:We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella. Electron microscopy revealed that vB_ButM_GuL6 belongs to the family Myoviridae, order Caudovirales. The genome of vB_ButM_GuL6 is a linear, circularl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04780-7
更新日期：2020-11-01 00:00:00
