Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains.

Abstract:

:Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Gerna G,Sarasini A,Torsellini M,di Matteo A,Baldanti F,Parea M,Battaglia M

doi

10.1007/BF01317927

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

315-22

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

107

pub_type

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