Abstract：
:Mosquito-borne alphaviruses are widely distributed throughout the world, causing important human illnesses. Therefore, the development of methods to enable early diagnosis of infections by alphavirus is essential. We show here the development and evaluation of a quantitative real-time RT-PCR using genus-specific primers to the nsP1 viral gene of all mosquito-borne alphaviruses. The specificity and sensitivity of the assay were tested using seven alphaviruses and RNA transcribed from Venezuelan equine encephalitis virus. The detection limits of real-time RT-PCR ranged from 10 to 76 copies per ml. The melting temperature (TM) values for amplification of the alphavirus genomes were 83.05 °C and 85.28 °C. Interestingly, the assay suggested the possibility the arthritogenic alphaviruses with TM peaks of 84.83 to 85.28 °C and encephalitic alphaviruses of 83.34 °C to 84.68 °C could be discriminated both diseases. Real-time RT-PCR may prove very useful for the screening and preliminary diagnosis in outbreaks and surveillance studies as well as for measuring the viral load in pathogenesis studies.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Romeiro MF,de Souza WM,Tolardo AL,Vieira LC,Henriques DA,de Araujo J,Siqueira CE,Colombo TE,Aquino VH,da Fonseca BA,de Morais Bronzoni RV,Nogueira ML,Durigon EL,Figueiredo LTdoi
10.1007/s00705-016-3019-0subject
Has Abstractpub_date
2016-11-01 00:00:00pages
3171-7issue
11eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-3019-0journal_volume
161pub_type
杂志文章abstract：:Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profil...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04832-y
更新日期：2020-12-01 00:00:00
abstract：:A new virus belonging to the family Dicistroviridae was identified in the hibiscus-infesting cotton mealybug Phenacoccus solenopsis. Using high-throughput sequencing (HTS) on an Illumina HiSeq platform, a single contig of the complete genome sequence was assembled. The authenticity of the sequence obtained by HTS was ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04702-7
更新日期：2020-09-01 00:00:00
abstract：:Two Citrus exocortis viroid isolates (CEVd-s and CEVd-129) that induce severe and mild symptoms in Gynura aurantiaca, respectively, have been characterized. They present nucleotide sequences in the pathogenicity motifs P(L), C and P(R) similar to those of "Class A" and "Class B". Infectivity and symptom expression in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0958-5
更新日期：2007-01-01 00:00:00
abstract：:Echovirus 33 (E33) has been infrequently detected and is less frequently associated with clinical diseases when compared with other types of enteroviruses (EVs) in China. An outbreak of E33 was identified in four schools in Hunan Province, China, in June 2013. For laboratory diagnosis, throat swabs and/or serum specim...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2059-6
更新日期：2014-09-01 00:00:00
abstract：:Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutage...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1952-8
更新日期：2014-06-01 00:00:00
abstract：:African swine fever (ASF) a lethal, viral hemorrhagic disease of domestic pigs, first reported from East Africa in 1921, is still widespread in this region. In order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the C-terminal end of the p72 gene were determined for 77 ASF ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0602-1
更新日期：2005-12-01 00:00:00
abstract：:Senecavirus A (SVA) is associated with vesicular disease in swine and the acute death of neonatal piglets. Here, senecavirus A was isolated from a pig displaying vesicular disease in Northeast China. The virus was designated as SVA/HLJ/CHA/2016 and its full-length nucleotide sequence was determined and analyzed in com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3480-4
更新日期：2017-10-01 00:00:00
abstract：:Subnormal temperatures were found to depress the production of interferon by bovid herpesvirus 2 (BHV-2)-infected bovine testicular cells, bovine peripheral blood leukocytes, and bovine monocytes, as well as by BHV-2 antigen-stimulated immune peripheral blood leukocytes. Interferon titers generated at 30 degrees C wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311164
更新日期：1984-01-01 00:00:00
abstract：:Morphological, biological, serological, and molecular tests underpin the description of costus stripe mosaic virus (CoSMV) as a new member of the genus Potyvirus, family Potyviridae. Found affecting the native ornamental Costus spiralis in Brazil, the pathogen showed a severely restricted natural and experimental host...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04788-z
更新日期：2020-11-01 00:00:00
abstract：:Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne illness but a serious panzootic disease with the potential to be pandemic. In this study, broiler chickens were vaccinated with commercial H5N1 or H5N2 inactivated vaccines prior to being challenged with an HPAIV H5N1 (clad...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1242-x
更新日期：2012-05-01 00:00:00
abstract：:Recently we have described that the Herpes simplex virus (HSV)-induced thymidine kinase (TK) induces AMP- and ADP-dThd-5'-phosphotransferase activities. We now demonstrate the heterogeneity of the described activities in isoelectric focusing experiments and polyacrylamide gel electrophoresis. A TK--mutant of HSV type ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314875
更新日期：1982-01-01 00:00:00
abstract：:Viruses are ubiquitous in the biosphere and greatly affect the hosts they infect. It is generally accepted that members of every microbial taxon are susceptible to at least one virus, and a plethora of bacterial viruses are known. In contrast, knowledge of the archaeal virosphere is still limited. Here, a novel lytic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04120-w
更新日期：2019-03-01 00:00:00
abstract：:The oncogene E6 of human papillomavirus 8, which is associated with skin cancers in epidermodysplasia verruciformis, was transcribed and translated in vitro. The resulting 17 kDa protein did not bind to the cellular p53 in contrast to E6 of HPV16. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309654
更新日期：1992-01-01 00:00:00
abstract：:We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2024-4
更新日期：2014-08-01 00:00:00
abstract：:Eco RI fragment G of equine herpesvirus 4 strain 405/76 (EHV 4.405/76) is located at the left end of the unique short region close to or extending into the internal repeat region of the prototypic arrangement of the genome. The nucleotide sequence of two subclones designated HS and G 19, contiguous within Eco RI fragm...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309795
更新日期：1993-01-01 00:00:00
abstract：:The presence of transmitted HIV drug resistance (TDR) threatens the efficacy of antiretroviral treatment. We aimed to assess the changes in TDR prevalence over the last decade in Madrid, Spain, to verify the role of the patients' risk groups in these changes. We analysed the trends of TDR between 2000 and 2011 in a co...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1933-y
更新日期：2014-05-01 00:00:00
abstract：:We constructed a recombinant feline herpesvirus type 1 (FHV-1) which was deleted in a defined region (450 bp) within the thymidine kinase (TK) gene (C7301dlTK) [Yokoyama et al. (1995) J Vet Med Sci 57: 709-714]. In this report, we carried out two experiments to assess the pathogenicity and vaccine efficacy of the reco...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718312
更新日期：1996-01-01 00:00:00
abstract：:Avian Tembusu virus (ATV), an emerging virus that mainly infects laying and breeding ducks in China, has caused severe economic loss in duck industry. However, there have been no reports about host innate immune responses during ATV infection and its correlation with clinical signs or pathology. To identify the roles ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2621-x
更新日期：2016-01-01 00:00:00
abstract：:RNA viruses are classified by their genome polarity and replication strategies. Nucleotide composition and codon usage differ among virus groups, for instance positive-sense RNA (+ssRNA) viruses have higher GC-content than the other RNA virus groups. Codon usage of +ssRNA viruses is closer to humans showing significan...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3930-7
更新日期：2018-10-01 00:00:00
abstract：:Knowledge of coronaviruses in wild carnivores is limited. This report describes coronavirus genetic diversity, species specificity and infection prevalence in three wild African carnivores. Coronavirus RNA was recovered from fresh feces from spotted hyena and silver-backed jackal, but not bat-eared fox. Analysis of se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1562-x
更新日期：2013-04-01 00:00:00
abstract：:Hepatitis E is rare in Japan but is occurring more frequently than previously thought. To investigate whether de novo subclinical infection of hepatitis E virus (HEV) has recently increased in Japan, HEV RNA was assayed in serum samples obtained from 4019 Japanese voluntary blood donors with alanine aminotransferase (...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0996-z
更新日期：2007-01-01 00:00:00
abstract：:In this study, we isolated a novel virulent Escherichia phage, SRT7. Its genome is a double-stranded linear DNA molecule containing 39,883 bp. Direct terminal repeats with a length of 175 bp, are present at both ends of the genome. The G+C content is 50.54%. Forty-seven putative protein coding genes were identified. N...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04182-4
更新日期：2019-04-01 00:00:00
abstract：:Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3218-8
更新日期：2017-05-01 00:00:00
abstract：:Twenty TGEV isolates were compared by sequencing a 393-414 nucleotide stretch near the 5' end of the S gene, after amplification by RT-PCR. This part of the S gene is known to show considerable variation between porcine, canine and feline coronaviruses and is completely deleted from porcine respiratory coronaviruses. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050191
更新日期：1997-01-01 00:00:00
abstract：:Electron microscopy and prolonged incubation of cell cultures have revealed the presence of a papovavirus in two foetal rhesus monkey kidney cell lines, FRhK-4 and FRhK-6, which are used to grow hepatitis A virus. The papovavirus, designated FRKV, was present in culture fluids from both cell lines and in thin sections...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310654
更新日期：1984-01-01 00:00:00
abstract：:Two hundred forty million people are chronically infected with hepatitis B virus (HBV) worldwide. The rise of globalization has facilitated the emergence of novel HBV recombinants and genotypes. We evaluated HBV genotypes and recombinants, mutations associated with resistance to antivirals (AVs), progression of hepati...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3122-2
更新日期：2017-02-01 00:00:00
abstract：:cDNA clones spanning the entire region of the peplomer (S) gene of feline infectious peritonitis virus (FIPV) type I strain KU-2 were obtained and their complete nucleotide sequences were determined. A long open reading frame (ORF) encoding 1464 amino acid residues was found in the gene, which was 12 residues longer t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718424
更新日期：1995-01-01 00:00:00
abstract：:Molecular epidemiology can be used to identify human immunodeficiency virus (HIV) transmission clusters, usually using pol sequence for analysis. In the present study, we explored appropriate parameters to construct a simple network using HIV env and gag sequences instead of pol sequences for constructing a phylogenet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3485-z
更新日期：2017-11-01 00:00:00
abstract：:Sequence analysis of a 6.4 kb DNA region from the Spodoptera littoralis multinucleocapsid nucleopolyhedrovirus (SpliNPV) revealed a large open reading frame (ORF) encoding a predicted polypeptide of 998 amino acid (aa) residues with a molecular mass of 114.93 kDa, located between 47.2-52.3 m.u. on the SpliNPV genome. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170177
更新日期：2001-01-01 00:00:00
abstract：:Members of the family Closteroviridae have emerged as a major problem in agricultural crops in the past two decades. Diodia vein chlorosis virus (DVCV) is an understudied whitefly-transmitted closterovirus. Given the presence of the primary host for the virus in major agricultural production areas in the United States...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1055-3
更新日期：2011-11-01 00:00:00
