Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species.

Abstract:

:Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5'-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Vetukuri RR,Kalyandurg PB,Saripella GV,Sen D,Gil JF,Lukhovitskaya NI,Grenville-Briggs LJ,Savenkov EI

doi

10.1007/s00705-020-04832-y

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

2953-2959

issue

12

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-020-04832-y

journal_volume

165

pub_type

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