Abstract：
:Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutagenesis and ELISA to screen the amino acids surrounding position 470 recognized by the MAb 3/1 as a linear epitope. Ten amino acid residues involved in the reaction of NP with the MAb 3/1 and the MAb 469/6 were identified. Our data are the first to outline a compact site recognized by MAbs in the 3D structure of the influenza virus NP.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Varich NL,Sadykova GK,Prilipov AG,Kochergin-Nikitsky KS,Webster RG,Kaverin NVdoi
10.1007/s00705-013-1952-8subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1493-7issue
6eissn
0304-8608issn
1432-8798journal_volume
159pub_type
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