Abstract:
:A real-time PCR assay based on primer-probe energy transfer (PriProET) was developed to detect swine vesicular disease virus (SVDV). Specificity tests of SVDV and heterologous virus showed specific amplification of SVDV strains only. The amplification plot for the closely related Coxsackievirus B5 remained negative. The sensitivity of assay was five copies of viral genome equivalents. A key point of the assay is tolerance toward mutations in the probe region. Melting curve analysis directly after PCR, with determination of probe melting point, confirmed specific hybridisation of the SVDV strains. Eight of twenty SVDV strains tested, revealed shifted melting points that indicated mutations in the probe region. All predicted mutations were confirmed by nucleotide sequencing. With the PriProET system there is a chance to identify phylogenetically divergent strains of SVDV, which may appear negative in other probe-based real-time PCR assays. At the same time, any difference in melting points may provide an indication of divergence in the probe region. The high sensitivity, specificity, and tolerance toward mutations in the probe region of the SVDV PriProET assay may improve the early and rapid detection of a wide range of SVDV strains, allowing reduced turnaround time and the use of high-throughput, automated technology.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hakhverdyan M,Rasmussen TB,Thorén P,Uttenthal A,Belák Sdoi
10.1007/s00705-006-0817-9subject
Has Abstractpub_date
2006-12-01 00:00:00pages
2365-76issue
12eissn
0304-8608issn
1432-8798journal_volume
151pub_type
杂志文章abstract::A new aquareovirus was isolated from cultured Atlantic halibut (Hippoglossus hippoglossus) fry at a facility where massive mortalities had occurred during the start-feeding phase. The same virus was also detected in juveniles (about 10 grams) of the 2013 generation at two other production sites, but not in larger fish...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2235-8
更新日期:2015-01-01 00:00:00
abstract::Meningitis is a serious condition that affects the central nervous system. It is an inflammation of the meninges, which is the membrane that surrounds both the brain and the spinal cord. Meningitis can be caused by bacterial, viral, or fungal infections. Many viruses, such as enteroviruses, herpesviruses, and influenz...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-020-04891-1
更新日期:2021-01-03 00:00:00
abstract::Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3618-4
更新日期:2018-02-01 00:00:00
abstract::Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3260-1
更新日期:2017-07-01 00:00:00
abstract::Heterogeneity of 9 feline herpesvirus type 1 (FHV-1) strains consisting of the prototype C27 strain, one French isolate, six Japanese isolates, and the attenuated vaccine F2 strain was examined by biological, immunological, and molecular biological methods. No significant difference was observed in virus growth and an...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309701
更新日期:1992-01-01 00:00:00
abstract::Betanodaviruses, members of the family Nodaviridae, have small positive-stranded bipartite RNA genomes and are the causal agent of viral nervous necrosis (VNN) in many species of marine farmed fish. In the aquaculture industry, outbreaks of betanodavirus infection and spread in larval and juvenile fish result in devas...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1061-7
更新日期:2007-01-01 00:00:00
abstract::The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5' and 3' untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins enco...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3147-6
更新日期:2017-03-01 00:00:00
abstract::The complete coding sequences were determined for four putative vesiculoviruses isolated from fish. Sequence alignment and phylogenetic analysis based on the predicted amino acid sequences of the five main proteins assigned tench rhabdovirus and grass carp rhabdovirus together with spring viraemia of carp and pike fry...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1711-x
更新日期:2013-11-01 00:00:00
abstract::The synthesis of M protein of HVJ (Sendai virus) in rat glioma (C6) cells, as has previously been reported, is selectively reduced at a nonpermissive temperature of 39 degrees C. In this phenomenon no difference was found in the viral RNA synthesis and the stability of the viral RNAs between the permissive and nonperm...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310830
更新日期:1988-01-01 00:00:00
abstract::A papovavirus was isolated from the urine of a 24 year old female who underwent bone marrow transplantation after a relapse of acute myeloid leukemia. The virus (JL) resembles BK virus in its antigenic and growth properties, but has a different restriction endonuclease pattern after digestion with the restriction endo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315635
更新日期:1978-01-01 00:00:00
abstract::Human bocavirus (HBoV) has been detected primarily in children with acute lower respiratory tract disease (LRTD), but its occurrence, clinical profile, and role as a causative agent of RTD are not clear. The aim of this study was to investigate the prevalence and the potential clinical relevance of HBoV. Using molecul...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04396-6
更新日期:2019-12-01 00:00:00
abstract::A potyvirus (isolate PFV-FJ) infecting passion fruit in China was identified by small-RNA sequencing. The complete genome sequence of PFV-FJ was determined to be 9974 nucleotides, excluding the poly(A) tail. PFV-FJ shares 70-72% nucleotide and 69-74% amino acid sequence identity at the polyprotein level with seven rep...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04251-8
更新日期:2019-07-01 00:00:00
abstract::Brugmansia suaveolens mottle virus (BsMoV) was the first potyvirus isolated from "angel trumpet" (Brugmansia suaveolens), described in Brazil. In this study, the complete nucleotide (nt) genome sequence of BsMoV was determined, and the deduced amino acid (aa) sequence was analyzed. The BsMoV RNA genome consists of 987...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0798-6
更新日期:2010-10-01 00:00:00
abstract::Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314741
更新日期:1980-01-01 00:00:00
abstract::Non-nucleoside reverse transcriptase inhibitors (NNRTIs) act quite specifically on human immunodeficiency virus type 1 (HIV-1). In general, they are not effective on human immunodeficiency virus type 2 (HIV-2) or simian immunodeficiency virus (SIV). Only SIV strains from African green monkeys are sensitive to several ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050070003
更新日期:2000-01-01 00:00:00
abstract::A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio pha...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04535-4
更新日期:2020-04-01 00:00:00
abstract::The presence of transmitted HIV drug resistance (TDR) threatens the efficacy of antiretroviral treatment. We aimed to assess the changes in TDR prevalence over the last decade in Madrid, Spain, to verify the role of the patients' risk groups in these changes. We analysed the trends of TDR between 2000 and 2011 in a co...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1933-y
更新日期:2014-05-01 00:00:00
abstract::Porcine endogenous retroviruses (PERV) are widely distributed in the genomes of pigs. PERV-A and PERV-B are present in all pigs. They infect human cells in vitro and therefore represent a risk for xenotransplantation when pig cells, tissues or organs are used. PERV-C infects only pig cells and is not present in the ge...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1490-9
更新日期:2013-02-01 00:00:00
abstract::Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne illness but a serious panzootic disease with the potential to be pandemic. In this study, broiler chickens were vaccinated with commercial H5N1 or H5N2 inactivated vaccines prior to being challenged with an HPAIV H5N1 (clad...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1242-x
更新日期:2012-05-01 00:00:00
abstract::During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0105-y
更新日期:2008-01-01 00:00:00
abstract::Echovirus 33 (E33) has been infrequently detected and is less frequently associated with clinical diseases when compared with other types of enteroviruses (EVs) in China. An outbreak of E33 was identified in four schools in Hunan Province, China, in June 2013. For laboratory diagnosis, throat swabs and/or serum specim...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2059-6
更新日期:2014-09-01 00:00:00
abstract::Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradeca...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310506
更新日期:1990-01-01 00:00:00
abstract::In our attempt to isolate mutant cell lines resistant to Newcastle disease virus (NDV) we developed an improved procedure for enrichment of NDV-resistant cells from mouse FM3A cells and isolated a novel NDV-resistant mutant cell line, Had-2, with characteristics different from Had-1, a previously reported NDV-receptor...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310796
更新日期:1994-01-01 00:00:00
abstract::During aetiological study of diseased red clover (Trifolium pratense L.) using high throughput sequencing, a novel virus with a 10 kb genome divided into two segments was discovered. The virus, tentatively named red clover associated varicosavirus (RCaVV), is phylogenetically related to classifiable members of the gen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3838-2
更新日期:2018-08-01 00:00:00
abstract::Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for the presence of anti-aHEV antibodies. In a random serology study...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-4089-y
更新日期:2019-02-01 00:00:00
abstract::Porcine circovirus type 2 (PCV2) is the essential infectious agent causing porcine circovirus-associated disease (PCVD) in pigs and one of the important viruses that severely jeopardize the swine husbandry industry. PCV2 elicits the unfolded protein response (UPR) via activation of the PERK pathway, and its capsid pro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04185-1
更新日期:2019-05-01 00:00:00
abstract::Modern adjuvants should induce strong and balanced immune responses, and it is often desirable to induce specific types of immunity. As an example, efficient Th1-immunity-inducing adjuvants are highly in demand. Such adjuvants promote good cell-mediated immunity against subunit vaccines that have low immunogenicity th...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-010-0863-1
更新日期:2011-02-01 00:00:00
abstract::The ability of lymphocytes from patients with multiple sclerosis (MS) to produce Interferon (IFN-alpha) in response to Newcastle Disease Virus (NDV) was studied in vitro. The correlation between individual IFN-alpha titers and natural killer (NK) cell activity and the presence of HLA system antigens associated with MS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314877
更新日期:1982-01-01 00:00:00
abstract::Simian varicella virus (SVV) infection of non-human primates is used as a model to study the pathogenesis and latency of varicella-zoster virus (VZV), the etiological agent of chickenpox and shingles. Uracil DNA glycosylase (UDG) is a DNA repair enzyme responsible for excision of uracil residues misincorporated into D...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050630
更新日期:1999-01-01 00:00:00
abstract::The present work shows that at least four different sequence types of Aleutian mink disease parvovirus (ADV) are present in ADV isolates from mink. We here report the nucleotide sequences of these four types of ADV from nucleotide 123 to 2208 (map unit 3 to 46). This part of the genome encodes three non-structural (NS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379127
更新日期:1994-01-01 00:00:00