Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus.

Abstract:

:Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330.8 kDa) in the vc strand. Analyses of the NSm, Gn/Gc and RdRp of PCSV revealed that PCSV is phylogenetically clustered within the watermelon silver mottle virus-related clade. Based on the whole genome sequence, PCSV is closely related to Tomato necrotic ringspot virus and should be classified as a new tospovirus species.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Huang KS,Tai CH,Cheng YH,Lin SH,Chen TC,Jan FJ

doi

10.1007/s00705-017-3260-1

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

2109-2113

issue

7

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-017-3260-1

journal_volume

162

pub_type

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