Abstract：
:A virus isolated from Olea europaea L. grown in Portugal, was identified as a member of the species Tobacco necrosis virus D (TNV-D, genus Necrovirus, family Tombusviridae), based on the molecular and serological properties of the purified virus particles. The genomic region encoding the coat protein (CP) of this isolate (named GP isolate) was amplified by RT-PCR and the cDNA was cloned and sequenced. The CP gene encodes a predicted protein of 269 amino acids showing high identity (86.2%) to TNV-D coat protein sequence. Phylogenetic analysis based on necroviruses CP sequences, confirmed GP as a TNV-D isolate. The alignment with homologous TNV-D CP sequences revealed four conserved amino acids involved in Ca(2+) binding as well as the plant virus icosahedral capsid protein "S' signature. Based on the determined nucleotide sequence, specific primers were designed and successfully used in RT-PCR for virus diagnosis in naturally infected olive trees.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Cardoso JM,Félix MR,Oliveira S,Clara MIdoi
10.1007/s00705-003-0258-7keywords:
subject
Has Abstractpub_date
2004-06-01 00:00:00pages
1129-38issue
6eissn
0304-8608issn
1432-8798journal_volume
149pub_type
杂志文章abstract：:DNA of TT virus (TTV), a novel human circovirus, was tested for in 116 mother-infant pairs who had participated in the adult T-cell leukemia prevention program (APP) in Nagasaki, Japan, and refrained from breast-feeding. By polymerase chain reaction with Okamoto's seminested primers, 36 of the 115 (31%) mothers were p...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050044
更新日期：2000-01-01 00:00:00
abstract：:The adsorption and retention properties of native (unfixed) and glutaraldehyde-fixed alfalfa mosaic virus (AMV) antigens to the polystyrene of ELISA plates were studied using [35S]-labelled virus preparations. It was shown that adsorption was a temperature-dependent, relatively slow process which varied between differ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310767
更新日期：1991-01-01 00:00:00
abstract：:A collection of rotavirus samples collected over a 26-year period was examined to study the dynamics of change in RV strains of genotype P[8] in a geographically defined population (Nizhny Novgorod, Russia; children under 6 years) with no vaccine pressure. Phylogenetic analysis of gene VP4 (subunit VP8*) showed the pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1426-4
更新日期：2012-12-01 00:00:00
abstract：:In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24.6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1424-6
更新日期：2012-12-01 00:00:00
abstract：:Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profil...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04832-y
更新日期：2020-12-01 00:00:00
abstract：:The oncogene E6 of human papillomavirus 8, which is associated with skin cancers in epidermodysplasia verruciformis, was transcribed and translated in vitro. The resulting 17 kDa protein did not bind to the cellular p53 in contrast to E6 of HPV16. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309654
更新日期：1992-01-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection has a major effect on health care systems, with about one-third of the world's population currently infected with the virus. There is an effective vaccine against HBV, which contains a recombinant "surface antigen" produced in an expression vector. Vaccination has proved to be success...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0206-7
更新日期：2008-01-01 00:00:00
abstract：:Astroviruses are associated with acute gastroenteritis of human and many animal species. Recently, two studies have reported that novel astroviruses were found in bats. In order to extensively understand the genetic and phylogenetic characterization of bat astroviruses, we tested fecal samples of nine bat species that...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1011-2
更新日期：2011-08-01 00:00:00
abstract：:The small intestine of piglets orally infected with rotavirus was examined by electron microscopy 18, 24, 48 and 60 hours after infection. At 18 and 24 hours after infection columnar epithelial cells covered the villi. Infected epithelial cells tended to be less electron-dense than uninfected cells and were more numer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317902
更新日期：1979-01-01 00:00:00
abstract：:Genomic characterization of Norwalk-like human caliciviruses (NLVs) originating from outbreaks and sporadic cases of acute gastroenteritis has revealed surprisingly high levels of diversity, even in the RNA polymerase gene, which is anticipated to be highly conserved. Since information on antigenic relationship is lim...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050020
更新日期：2000-01-01 00:00:00
abstract：:The in vitro cytopathology and the neurovirulence to inbred mice demonstrated by five variants originally derived from one laboratory strain (Miyama) of type 1 herpes simplex virus (HSV-1) were studied comparatively. Three of the variants are syncytial [+GC (LPV), +GC (SPV), +GC (81)] and two are non-syncytial [-GCr a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317369
更新日期：1986-01-01 00:00:00
abstract：:The movement protein (MP) of tobacco mosaic virus (TMV) mediates the transport of viral RNA from infected cells to neighboring uninfected cells via plasmodesmata by interacting with putative host factors. To find such host factors, we screened tobacco proteins using the yeast two-hybrid system. NtMPIP1, a novel subset...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0397-6
更新日期：2009-01-01 00:00:00
abstract：:cDNA clones spanning the entire region of the peplomer (S) gene of feline infectious peritonitis virus (FIPV) type I strain KU-2 were obtained and their complete nucleotide sequences were determined. A long open reading frame (ORF) encoding 1464 amino acid residues was found in the gene, which was 12 residues longer t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718424
更新日期：1995-01-01 00:00:00
abstract：:3Y1 rat fibroblasts transformed by adenovirus type 12 or by its E1A gene formed syncytia when cocultivated with the Friend as well as other ecotropic murine leukemia-virus-producing cells. In contrast, the parental 3Y1 cells and those transformed by other tumor viruses or by a chemical carcinogen exhibited no syncytiu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310628
更新日期：1990-01-01 00:00:00
abstract：:The receptor for adeno-associated virus serotype 2 (AAV2) in Purkinje cells has not been identified, but based on work carried out in non-neuronal cell lines, heparan sulfate proteoglycan is thought to act as a primary receptor, with basic fibroblast growth factor receptor-1 being reported as a co-receptor. In this st...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0222-7
更新日期：2008-01-01 00:00:00
abstract：:Rice black-streaked dwarf virus (RBSDV) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in East Asia. To study RBSDV variation and recombination, we examined the segment 9 (S9) sequences of 49 RBSDV isolates from maize and rice in China. Three S9 recombina...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2291-0
更新日期：2015-04-01 00:00:00
abstract：:Cellular uptake of fowl plague virus occurs 10-30 minutes after inoculation of chick embryo cells. The penetration of the virions is by pinocytosis (viropexis); fusion with the cellular membrane has not been observed. After pinocytosis the virions become gradually disintegrated. Budding of newly formed virions from th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317537
更新日期：1975-01-01 00:00:00
abstract：:Although surfactant-secreting type II alveolar cells have been shown to be damaged during influenzavirus pneumonia, little is known about the effects of surfactant replacement therapy. We have developed a mouse influenza model, in which viral infection can be localized to the upper respiratory tract or to both the upp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050535
更新日期：1999-01-01 00:00:00
abstract：:We constructed a recombinant feline herpesvirus type 1 (FHV-1) which was deleted in a defined region (450 bp) within the thymidine kinase (TK) gene (C7301dlTK) [Yokoyama et al. (1995) J Vet Med Sci 57: 709-714]. In this report, we carried out two experiments to assess the pathogenicity and vaccine efficacy of the reco...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718312
更新日期：1996-01-01 00:00:00
abstract：:The value of serologic tests for diagnosis of swine influenza virus (SIV) infection has been diminished by the emergence of new subtypes and by antigenic drift within subtype. The intensive use of vaccination also has complicated interpretation of serology results. Serologic assays are needed that can detect infection...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0615-9
更新日期：2006-01-01 00:00:00
abstract：:In a virus-infected plant, small interfering RNAs (siRNAs) corresponding to the viral genome form a large proportion of the small RNA population. It is possible to reassemble significant portions of the virus sequence from overlapping siRNA sequences and use these to identify the virus. We tested this technique with a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0979-y
更新日期：2011-07-01 00:00:00
abstract：:Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial morphology and dist...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3890-y
更新日期：2018-10-01 00:00:00
abstract：:To elucidate changes associated with the attenuated virulence in a modified live porcine reproductive and respiratory syndrome (PRRS) vaccine (Boehringer Ingelheim Animal Health, St. Joseph, MO), derived from an American prototype ATCC virus VR-2332, nucleotide sequence of 3' genome covering open reading frames (ORFs)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050316
更新日期：1998-01-01 00:00:00
abstract：:IG 1, a temperate phage of Bacillus subtilis, is strongly induced from its lysogens by 6-(p-hydroxyphenylazo)-uracil, an azopyrimidine known as selectively inhibiting the B. subtilis DNA polymerase III. IG 1 phages originated either by induction or infection multiply, abundantly, in the presence of that azopyrimidine,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316671
更新日期：1990-01-01 00:00:00
abstract：:Virus-like particles (VLP), morphologically similar to togavirus particles, were detected in the sera of chimpanzees and humans with chronic non-A, non-B (NANB) hepatitis. Particles resembling the virus were also found in the cytoplasm of hepatocytes obtained from NANB hepatitis infected chimpanzees. Furthermore, two ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315557
更新日期：1989-01-01 00:00:00
abstract：:Peach asteroid spot (PAS) and peach sooty ringspot (PSRS) are two diseases of stone fruit trees of unknown aetiology. The use of a cRNA probe of the newly described Apricot latent virus (ApLV), a tentative member of the Foveavirus genus, indicated the presence of cross-hybridizing agents in PAS isolate LA2 and in PSRS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170071
更新日期：2001-08-01 00:00:00
abstract：:Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, hum...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0396-7
更新日期：2009-01-01 00:00:00
abstract：:Sixteen patients with symptoms typical for Ockelbo disease (rash, arthralgia, fever) were enrolled in a 2 1/2 year study, during which clinical symptoms were recorded and ELISA was employed to study specific IgM, IgG and IgG subclass development. Initially, all patients presented with rash and arthralgia, and five pat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379107
更新日期：1994-01-01 00:00:00
abstract：:Heterogeneity of 9 feline herpesvirus type 1 (FHV-1) strains consisting of the prototype C27 strain, one French isolate, six Japanese isolates, and the attenuated vaccine F2 strain was examined by biological, immunological, and molecular biological methods. No significant difference was observed in virus growth and an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309701
更新日期：1992-01-01 00:00:00
abstract：:JC virus (JCV) strains worldwide can be classified into various genotypes based on DNA sequence variations. To define the domains of the four major JCV genotypes in Asia, we collected urine samples at six unstudied sites: three in southeastern Asia, two in the central highlands and one in central Asia. DNA was extract...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8299-4
更新日期：2002-01-01 00:00:00
