Electron microscopical study of initial and final stages of fowl plague virus-replication in chick embryo cells.

Abstract:

:Cellular uptake of fowl plague virus occurs 10-30 minutes after inoculation of chick embryo cells. The penetration of the virions is by pinocytosis (viropexis); fusion with the cellular membrane has not been observed. After pinocytosis the virions become gradually disintegrated. Budding of newly formed virions from the cellular membrane starts 3 hours post inoculation (p.i.) and reaches its maximum 8 hours p.i. At the same time budding takes place into electron microscopically empty and autophagic vacuoles. Eight hours p.i. about 3 per cent of the infected cells show budding of virions from the surface and into cytoplasmic vacuoles. Labelling of the cellular membrane with ruthenium red demonstrated that these cytoplasmic vacuoles are not simple cross-sections of invaginations of the cellular membrane. Cluster-like structures were found at 6 hours p.i. in the nuclei of infected cells; however, the suggestion that the clusters develop from nucleoli could not be confirmed.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Reinacher M,Weiss E

doi

10.1007/BF01317537

keywords:

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

187-97

issue

2-3

eissn

0304-8608

issn

1432-8798

journal_volume

49

pub_type

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