Physiochemical and immunological characterization of hepatitis A virus nucleocapsids expressed in a vaccinia virus/T7/EMCV system.

Abstract:

:Expression of the entire open reading frame of hepatitis A virus was achieved in a vaccinia virus/T7/EMCV hybrid system. The expressed antigens were characterized as particles with buoyant densities and sedimentation coefficients typical of empty capsids and pentamers of HAV. This was further confirmed by electron microscopy. All capsid proteins were determined as components of the particles which raised neutralizing antibodies in an immunized rabbit.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Zhu NL,Li GD,Wang Y

doi

10.1007/BF01310029

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

443-9

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

135

pub_type

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