Abstract：
:Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%. In a clinical sample evaluation, this approach demonstrated sufficient clinical performance when compared with a commercial RT-qPCR diagnostic kit. Thus, the RT-RPA assay may be a promising alternative for the detection of EV71-C4.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Yin D,Zhu Y,Wang K,Wang J,Zhang X,Han M,He Y,Chen Q,Hu Gdoi
10.1007/s00705-018-3859-xsubject
Has Abstractpub_date
2018-09-01 00:00:00pages
2459-2463issue
9eissn
0304-8608issn
1432-8798pii
10.1007/s00705-018-3859-xjournal_volume
163pub_type
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