Abstract：
:Bacterial endotoxins, DNA, live viruses, and viral proteins derived from bacterial and baculovirus (BV) expression vectors employed in recombinant protein production contaminate the final product. Density gradient centrifugation is commonly used to partially purify oligomeric proteins, but impurities from the expression system still remain. We describe a simple and rapid ultrafiltration method for final purification of rotavirus VP6 oligomeric nanotubes and nanospheres. Contamination originating from the BV vector used in VP6 production was undetectable. The method is highly efficient, fast and inexpensive and can be used for a small-scale laboratory purification of VP6 protein to replace technically demanding multi-step chromatographic procedures.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Lappalainen S,Vesikari T,Blazevic Vdoi
10.1007/s00705-016-2991-8subject
Has Abstractpub_date
2016-11-01 00:00:00pages
3219-23issue
11eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-2991-8journal_volume
161pub_type
杂志文章abstract：:Mouse hepatitis virus 3 (MHV-3) is highly hepatotropic in sensitive mice. Temperature-sensitive mutants (ts mutants) induced by N-methyl-N'-nitrosoguanidine and 5-fluorouracil were isolated. Twelve mutants which were able to induce the formation of syncytia at 33 degrees C but not at the restrictive temperature (39.5 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01487679
更新日期：1988-01-01 00:00:00
abstract：:The distribution of African swine fever virus (ASFV) in whole blood, plasma, red blood cells (RBC) and white blood cell (WbC) sub-populations was determined in pigs infected with virulent virus. Changes in the RBC and WBC populations were also examined. Total WBC counts decreased and RBC numbers remained unchanged dur...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315054
更新日期：1977-01-01 00:00:00
abstract：:Rabbit convalescent and hyperimmune sera, human patient and blood donor sera, as well as cerebrospinal fluids of humans with herpes simplex virus encephalitis all recognize similar major antigenic components in herpes simplex virus infected rabbit or human cells as shown by electrophoretic analysis of immunoprecipitat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314855
更新日期：1977-01-01 00:00:00
abstract：:Euthymic (SD or outbred rnu/+) and athymic (rnu/rnu) rats were inoculated oronasally or intraperitoneally with the RV-Y strain of rat virus when they were 2 days or 4 weeks old. Clinical signs of infection in athymic infants were similar to those in euthymic infants, but significantly more athymic infants died. Some i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311362
更新日期：1989-01-01 00:00:00
abstract：:Virological surveillance is an important element in the Polio Eradication Initiative to provide information rapidly about circulating wild polioviruses. Molecular tools have been developed to identify the serotype of the poliovirus strains and whether they are of vaccine or wild origin (intratypic differentiation) and...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0001-x
更新日期：2008-01-01 00:00:00
abstract：:Cytomegalovirus (CMV) infections are common after transplantation, but usually successfully treated with antivirals. In this study, the detection of CMV-DNA in peripheral blood leukocytes was monitored and compared with CMVpp65-antigenemia in liver transplant patients receiving ganciclovir treatment. Twenty adult live...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0108-7
更新日期：2003-07-01 00:00:00
abstract：:Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3788-8
更新日期：2018-07-01 00:00:00
abstract：:The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degree...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309574
更新日期：1992-01-01 00:00:00
abstract：:A wheat-infecting furovirus found in Tompkins County, New York, U.S.A. was identified as a strain of Soil-borne wheat mosaic virus (SBWMV) by means of sequence analyses of portions of its RNA 1 and 2. The nucleotide sequences of several of its genes differed by c. 9 to 12% from those of the corresponding genome region...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200011
更新日期：2002-03-01 00:00:00
abstract：:The effect of the local anaesthetic drug procaine on the adsorption of two enveloped viruses was studied. Physiological concentrations of the drug (7 X 10(-3)--7 X 10(-2) M) strongly inhibited the adsorption of both West-Nile and herpes viruses as determined by plaque assay and the infective center assay. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317291
更新日期：1978-01-01 00:00:00
abstract：:Hemorrhagic fever with renal syndrome (HFRS) is endemic in East Asia and Europe. The disease is caused by several viruses belonging to the genus Hantavirus, including the Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava Belgrade virus (DOBV), and Puumala virus (PUUV). Recently, HTNV-related viruses, Amur (AMR) and Fa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0113-x
更新日期：2003-08-01 00:00:00
abstract：:In dengue virus infected Aedes albopictus cells, electron-dense particles, larger than single ribosomes, were arranged on the cytoplasmic sides of rough endoplasmic reticulum (RER) membranes. Mature virions 40--45 nm in diameter as well as vesiculotubular structures 50--120 nm in diameter appeared in enlarged cisterna...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314902
更新日期：1979-01-01 00:00:00
abstract：:For selecting the neutralizing monoclonal antibodies (N-MAbs) directed to VP4 of rotavirus strain K8, which has unique VP4 neutralization epitopes, we prepared several reassortant viruses by mixed infection of two different strains K8 (serotype 1) and P (serotype 3) in vitro: three reassortant clones having VP4 of K8 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316751
更新日期：1991-01-01 00:00:00
abstract：:Lymphocytic chorimeningitis virus (LCMV), the prototype arenavirus, and Lassa virus (LASV), causative agent of Lassa hemorrhagic fever (LHF), belong to the Old World group of the family Arenaviridae. Both viruses have extensive strain diversity and significant variations in lethality and pathogenicity for man and expe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0385-9
更新日期：2004-12-01 00:00:00
abstract：:Sweet potato mild mottle virus (SPMMV) is the type member of the genus Ipomovirus (family Potyviridae) and is only known to occur in East Africa. In Uganda, SPMMV is the third most prevalent virus infecting sweet potato. The sequence variability of SPMMV was studied by cloning and sequencing a 1.8-kb fragment represen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0930-3
更新日期：2003-03-01 00:00:00
abstract：:A new human endogenous retroviral family (HERV-F) has recently been identified from human chromosome 7q31.1-q31.3 that was identical to the XA34 cDNA clone isolated from a human glioma cDNA library with an ERV-9 env probe. We investigated pol fragments of the HERV-F family from the Old World monkeys (crab-eating monke...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8327-7
更新日期：2002-01-01 00:00:00
abstract：:A new virus belonging to the family Dicistroviridae was identified in the hibiscus-infesting cotton mealybug Phenacoccus solenopsis. Using high-throughput sequencing (HTS) on an Illumina HiSeq platform, a single contig of the complete genome sequence was assembled. The authenticity of the sequence obtained by HTS was ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04702-7
更新日期：2020-09-01 00:00:00
abstract：:An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P(late), hydroxylamine mutagenesis was pe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0851-7
更新日期：2007-02-01 00:00:00
abstract：:Monitoring of influenza A virus (IAV) was conducted in wild bird species in central Thailand. Four IAV subtype H12N1 strains were isolated from a watercock (order Gruiformes, family Rallidae) (n = 1) and lesser whistling ducks (order Anseriformes, family Anatidae) (n = 3). All H12N1 viruses were characterized by whole...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1260-8
更新日期：2012-06-01 00:00:00
abstract：:Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2473-4
更新日期：2015-08-01 00:00:00
abstract：:We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, in breeding lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) collected from the two centers of common bean domestication: Mesoamerica and the Andes. Th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2335-0
更新日期：2015-04-01 00:00:00
abstract：:Acute diarrhea outbreaks caused by porcine epidemic diarrhea virus (PEDV) have been observed in various pig-breeding provinces of China since December 2010. Endemic strains of PEDV were isolated from different areas, and the complete genome sequences of 10 isolates were determined. Our objective in this study was to g...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1767-7
更新日期：2013-12-01 00:00:00
abstract：:Infectious bursal disease virus (IBDV), a member of the family Birnaviridae, is a non-enveloped, double-stranded RNA virus. Viral protein 1 (VP1), the putative RNA-dependent RNA polymerase, occurs in virions both as a free polypeptide and as a genome-linked protein, called VPg. To gain more insight in its function, we...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0365-0
更新日期：2004-11-01 00:00:00
abstract：:We determined the complete genome sequence of the passion fruit woodiness virus Gld-1 isolate (PWV-Gld-1) from Australia and compared it with that of PWV-MU-2, another Australian isolate of PWV. The genomes shared high sequence identity in both the complete nucleotide sequence and the ORF amino acid sequence. All of t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1670-2
更新日期：2013-08-01 00:00:00
abstract：:Colons from ducks and chicken 1, 7, 14 and 28 days old maintained near-normal morphology up to 48 and 96 hours respectively in a system using NCTC135 medium (a part) + Dulbecco's modified Eagle's medium (9 parts ), at 37 degrees C and 95 per cent O2/5 per cent CO2. In the colon of 1 and 28 day-old ducks, duck influenz...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315007
更新日期：1981-01-01 00:00:00
abstract：:The complete nucleotide sequence of Subterranean clover mottle virus (SCMoV) genomic RNA has been determined. The SCMoV genome is 4,258 nucleotides in length. It shares most nucleotide and amino acid sequence identity with the genome of Lucerne transient streak virus (LTSV). SCMoV RNA encodes four overlapping open rea...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0144-3
更新日期：2003-11-01 00:00:00
abstract：:The Sindbis replicon expression system was used to express La Crosse (LAC) virus envelope glycoprotein genes in both mammalian and mosquito cell culture. Replicon expressed LAC proteins had correct molecular mass (Mr) and were antigenically similar to wild type LAC envelope proteins. In addition, LAC G1 and G2 protein...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050381
更新日期：1998-01-01 00:00:00
abstract：:In addition to acute infection and disease, foot-and-mouth disease virus (FMDV) can cause persistent infection in ruminants. Such "carrier" animals represent a potential risk for FMDV transmission to susceptible animals. However, the mechanisms and the factors that determine FMDV persistence remain unknown. We describ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2526-8
更新日期：2015-10-01 00:00:00
abstract：:A rice protein homologous to eukaryotic translation elongation factor 1A (eEF-1A) was found to interact with the Pns6 of rice ragged stunt virus (RRSV), the type member of the genus Oryzavirus, family Reoviridae, in yeast two-hybrid screening. The interaction between the rice protein, designated OseEF-1A, and RRSV Pns...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1703-x
更新日期：2013-10-01 00:00:00
abstract：:The DECS (dsRNA isolation, exhaustive amplification, cloning and sequencing) analysis technique for viral diagnosis detected a tombusvirus in Japanese gentian not displaying severe symptoms. We tentatively named this virus "gentian virus A" (GeVA). GeVA systemically but inefficiently infected Japanese gentian without ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3888-5
更新日期：2018-09-01 00:00:00
