Abstract:
:Mouse hepatitis virus 3 (MHV-3) is highly hepatotropic in sensitive mice. Temperature-sensitive mutants (ts mutants) induced by N-methyl-N'-nitrosoguanidine and 5-fluorouracil were isolated. Twelve mutants which were able to induce the formation of syncytia at 33 degrees C but not at the restrictive temperature (39.5 degrees C) were selected for detailed study. No viral RNA synthesis was detected after infection at the restrictive temperature with six of the mutants (RNA-) whereas six others were RNA+, although they displayed RNA synthesis which was generally reduced. No differences have been detected in the size of the genome or the viral-intracellular RNA species found in wild type virus or ts mutant infected cells at permissive temperature. The pattern of virus-induced proteins analyzed after immunoprecipitation by SDS-PAGE was similar in wild type virus and RNA+ mutant infected cells at 39.5 degrees C. Complementation experiments between ts mutants enabled us to distinguish five groups. Three of the groups contained RNA- mutants and two of them RNA+. Plaques made by mutants in one group displayed characteristic features that distinguished them from the wild type.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Martin JP,Koehren F,Rannou JJ,Kirn Adoi
10.1007/BF01487679subject
Has Abstractpub_date
1988-01-01 00:00:00pages
147-60issue
3-4eissn
0304-8608issn
1432-8798journal_volume
100pub_type
杂志文章abstract::Swine testicle cell lines were established by transformation of primary swine testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid DNA was transfected into PST cells using a lipofection system. Two related plasmids, pS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310532
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journal_title:Archives of virology
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更新日期:2017-05-01 00:00:00
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更新日期:2007-01-01 00:00:00
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更新日期:2006-02-01 00:00:00
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更新日期:1993-01-01 00:00:00
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更新日期:2020-02-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314635
更新日期:1992-01-01 00:00:00
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更新日期:2015-11-01 00:00:00
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