Pathogenicity and vaccine efficacy of a thymidine kinase gene deleted infectious laryngotracheitis virus expressing the green fluorescent protein gene.


:The deletion of the thymidine kinase (TK) gene of herpesviruses causes a reduction in their virulence. However, the effects of the TK gene in infectious laryngotracheitis virus (ILTV) have not been clearly elucidated. In the present study, we constructed a TK gene-deleted recombinant ILTV expressing the green fluorescent protein (GFP) gene as a marker. The GFP gene was inserted in place of the TK gene in both virulent and low virulence strains of ILTV. The GFP gene in the recombinants was expressed in chicken kidney cells, LMH cells and in the chorioallantoic membrane of embryonated chicken eggs. The recombinants produced cytopathic effects in chicken kidney cells and LMH cells and formed pocks in the chorioallantoic membrane of embryonated chicken eggs. The growth rate of the recombinant in chicken kidney cells was similar to that of wild type viruses. The recombinants showed a reduction of virulence compared to that of parental viruses and induced protection against virulent ILTV in specific pathogen free chickens. The recombinant expressing GFP gene may be a candidate for a genetically engineered vaccine and provide a means to study growth kinetics and mechanism of latent infection and reactivation of ILTV. In this study, we confirmed that the TK gene is directly related to virulence of ILTV. This is the first report to show the evidence that the TK gene is a major gene related to virulence of ILTV.


Arch Virol


Archives of virology


Han MG,Kweon CH,Mo IP,Kim SJ





Has Abstract


2002-05-01 00:00:00












  • Effect of gene constellation and postreassortment amino acid change on the phenotypic features of H5 influenza virus reassortants.

    abstract::Reassortants between a low-pathogenic avian influenza virus strain A/Duck/Primorie/2621/2001 (H5N2) and a high-yield human influenza virus strain A/Puerto Rico/8/34 (H1N1) were generated, genotyped and analyzed with respect to their yield in embryonated chicken eggs, pathogenicity for mice, and immunogenicity. A reass...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Rudneva IA,Timofeeva TA,Shilov AA,Kochergin-Nikitsky KS,Varich NL,Ilyushina NA,Gambaryan AS,Krylov PS,Kaverin NV

    更新日期:2007-01-01 00:00:00

  • Improved method for the purification of hog cholera virus grown in tissue culture.

    abstract::A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Laude H

    更新日期:1977-01-01 00:00:00

  • Expression, cellular localization and antibody responses of the African swine fever virus genes B602L and K205R.

    abstract::Previously, we identified serological immunodeterminants of African swine fever virus (ASFV), including pK205R and pB602L, without homologues in the database. pK205R is expressed as a 33-kD protein from 4 h post-infection onward, initially diffusely distributed throughout cells, and subsequently in viral factories. pK...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Gutiérrez-Castañeda B,Reis AL,Corteyn A,Parkhouse RM,Kollnberger S

    更新日期:2008-01-01 00:00:00

  • Some observations on the binding properties of alfalfa mosaic virus to polystyrene and its significance to indirect ELISA.

    abstract::The adsorption and retention properties of native (unfixed) and glutaraldehyde-fixed alfalfa mosaic virus (AMV) antigens to the polystyrene of ELISA plates were studied using [35S]-labelled virus preparations. It was shown that adsorption was a temperature-dependent, relatively slow process which varied between differ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Hajimorad MR,Francki RI

    更新日期:1991-01-01 00:00:00

  • Replication of cytomegalovirus in human epitheloid diploid cell line.

    abstract::Human diploid BAMB cells with epitheloid morphology, which had been derived from amniotic fluid cells, were capable of supporting the replication of human cytomegalovirus (CMV), without prior treatment of the cells with halogenated pyrimidines. The growth of this virus in BAMB cells and in human diploid fibroblastoid ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Vonka V,Anisimová E,Macek M

    更新日期:1976-01-01 00:00:00

  • Different mechanisms of synthesis of complete and incomplete influenza virus transcripts in infected cells.

    abstract::Two types of transcripts, incomplete (polyadenylated virus-specific mRNAs) and complete (presumably templates for progeny virus replication) are synthesized in influenza virus infected cells (3). The synthesis of incomplete transcripts requires as a primer de novo synthesized host cell mRNAs (4). We show here that the...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Staroff A,Bukrinskaya A

    更新日期:1981-01-01 00:00:00

  • Rabovirus: a proposed new picornavirus genus that is phylogenetically basal to enteroviruses and sapeloviruses.

    abstract::We have sequenced the genome of a novel picornavirus, rabovirus A (rat-borne virus, RaBoV-A, NC_026314), which was present in the feces of a Norway rat (Rattus norvegicus) from Berlin, Germany. This virus is related to members of the genera Enterovirus and Sapelovirus. RaboV-A contains a type II IRES that is unlike th...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ng TF,Sachsenröder J,Reuter G,Knowles NJ,Delwart E,Johne R

    更新日期:2015-10-01 00:00:00

  • B-cell activation in cats with feline infectious peritonitis (FIP) by FIP-virus-induced B-cell differentiation/survival factors.

    abstract::It has been suggested that antibody overproduction plays a role in the pathogenesis of feline infectious peritonitis (FIP). However, only a few studies on the B-cell activation mechanism after FIP virus (FIPV) infection have been reported. The present study shows that: (1) the ratio of peripheral blood sIg(+) CD21(-) ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Takano T,Azuma N,Hashida Y,Satoh R,Hohdatsu T

    更新日期:2009-01-01 00:00:00

  • Leakage of cellular enzyme during replication of oncogenic and nononcogenic adenoviruses.

    abstract::Leakage of glutamic-oxalacetic transaminase was monitored as an indicator of cell damage during a 48-hour period in which maximum virus replication occurred. There was increased leakage of the enzyme from HEp-2 cells infected with highly oncogenic adenovirus type 12. Leakage from cells infected with adenovirus types 1...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Bardell D

    更新日期:1983-01-01 00:00:00

  • Antiviral activity of baicalin against influenza A (H1N1/H3N2) virus in cell culture and in mice and its inhibition of neuraminidase.

    abstract::Scutellaria baicalensis Georgi, a Chinese herbal decoction, has been used for the treatment of the common cold, fever and influenza virus infections. In previous studies, we found that oral administration of baicalein resulted in the inhibition of influenza A virus replication in vivo, which was linked to baicalin in ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ding Y,Dou J,Teng Z,Yu J,Wang T,Lu N,Wang H,Zhou C

    更新日期:2014-12-01 00:00:00

  • Comparison of hemagglutinating, receptor-destroying, and acetylesterase activities of avirulent and virulent bovine coronavirus strains.

    abstract::Hemagglutinating and acetylesterase functions as well as the 124 kDa glycoprotein were present in the highly cell-culture adapted, avirulent bovine coronavirus strain BCV-L9, in the Norden vaccine strain derived from it, and in 5 wild-type, virulent strains that multiplied in HRT-18 cells but were restricted in severa...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Storz J,Zhang XM,Rott R

    更新日期:1992-01-01 00:00:00

  • Sequence analysis of the lymphotropic Aleutian disease parvovirus ADV-SL3.

    abstract::About 98% of the DNA sequence of the lymphotropic Aleutian disease parvovirus isolate ADV-SL3 was determined and analysed. The sequence revealed that this isolate was a type-1 ADV strain, supporting that the currently used typing of ADV viruses does not correlate with virulence or pathogenicity. ADV-SL3 had a very hig...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Schuierer S,Bloom ME,Kaaden OR,Truyen U

    更新日期:1997-01-01 00:00:00

  • A model for the membrane topology of the NS3 protein as predicted from the sequence of segment 10 of epizootic haemorrhagic disease virus serotype 1.

    abstract::Segment 10, encoding nonstructural proteins 3 (NS3) and 3a (NS3a) of epizootic haemorrhagic disease virus serotype 1 (EHDV-1) was sequenced. Computer motif recognition programs were used for interpretation of the sequence data to predict a structure for NS3. Integral membrane protein theories were then applied to prod...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Jensen MJ,Wilson WC

    更新日期:1995-01-01 00:00:00

  • Anti-viral activity against encephalomyocarditis virus and Semliki Forest virus and acute toxicity of poly I and poly C administered sequentially to mice.

    abstract::Mice are protected against lethal intraperitoneal and intravenous infection by encephalomyocarditis virus and Semliki Forest virus by sequential treatment with poly I followed by either polyC or poly5-hydroxyC without production of interferon when the treatments are 4 or more hours apart and by the intraperitoneal or ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Stebbing N,Grantham CA

    更新日期:1976-01-01 00:00:00

  • B-cell epitopes of varicella-zoster virus glycoprotein II.

    abstract::B-cell epitopes of varicella-zoster virus glycoprotein II were mapped by means of solid phase ELISA, synthetic oligopeptides (constructed according to the Davison-Scott sequencing of the varicella-zoster virus genome) and sera from varicellae and herpes zoster patients. The individual pattern of antibody peptide bindi...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kjartansdóttir A,Lycke E,Norrby SR

    更新日期:1996-01-01 00:00:00

  • Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species.

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    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Vetukuri RR,Kalyandurg PB,Saripella GV,Sen D,Gil JF,Lukhovitskaya NI,Grenville-Briggs LJ,Savenkov EI

    更新日期:2020-12-01 00:00:00

  • Occult hepatitis B virus infection in Korean patients with isolated anti-HBc.

    abstract::Serological detection of isolated anti-hepatitis B core antibody (anti-HBc) can occur in various scenarios, but the most clinically relevant situation is occult hepatitis B virus (HBV) infection (OBI). The purpose of this study was to evaluate the prevalence and clinical relevance of isolated anti-HBc and of OBI with ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kang SY,Kim MH,Lee WI

    更新日期:2014-02-01 00:00:00

  • The complete sequence of a Spanish isolate of Broad bean wilt virus 1 (BBWV-1) reveals a high variability and conserved motifs in the genus Fabavirus.

    abstract::The genome of a Spanish isolate of Broad bean wilt virus-1 (BBWV-1) was completely sequenced and compared with available sequences of other isolates of the genus Fabavirus (BBWV-1 and BBWV-2). This consisted of two RNAs of 5814 and 3431 nucleotides, respectively, and their organization was similar to that of other mem...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ferrer RM,Guerri J,Luis-Arteaga MS,Moreno P,Rubio L

    更新日期:2005-10-01 00:00:00

  • Genetic and phylogenetic analysis of a novel parvovirus isolated from chickens in Guangxi, China.

    abstract::A previously unidentified chicken parvovirus (ChPV) strain, associated with runting-stunting syndrome (RSS), is now endemic among chickens in China. To explore the genetic diversity of ChPV strains, we determined the first complete genome sequence of a novel ChPV isolate (GX-CH-PV-7) identified in chickens in Guang Xi...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Feng B,Xie Z,Deng X,Xie L,Xie Z,Huang L,Fan Q,Luo S,Huang J,Zhang Y,Zeng T,Wang S,Wang L

    更新日期:2016-11-01 00:00:00

  • A report on the outbreak of Zika virus on Easter Island, South Pacific, 2014.

    abstract::Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. In 2013, a large outbreak was reported on the archipelago of French Polynesia. In this study, we report the detection and molecular characterization of Zika virus for the first time in Chile from an outbreak among the inhabitant...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Tognarelli J,Ulloa S,Villagra E,Lagos J,Aguayo C,Fasce R,Parra B,Mora J,Becerra N,Lagos N,Vera L,Olivares B,Vilches M,Fernández J

    更新日期:2016-03-01 00:00:00

  • Nucleotide sequence analysis of Vietnamese highly pathogenic porcine reproductive and respiratory syndrome virus from 2013 to 2014 based on the NSP2 and ORF5 coding regions.

    abstract::A total of 34 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strains isolated from Vietnam during 2013-2014 were sequenced and analyzed. A partial sequence of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and the full sequence of open reading frame 5 (ORF5) g...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Do DT,Park C,Choi K,Jeong J,Nguyen TT,Le DT,Vo KM,Chae C

    更新日期:2016-03-01 00:00:00

  • Preparation and characterization of a neutralizing monoclonal antibody directed to VP4 of rotavirus strain K8 which has unique VP4 neutralization epitopes.

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    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kobayashi N,Taniguchi K,Urasawa T,Urasawa S

    更新日期:1991-01-01 00:00:00

  • Wild-type temperature-sensitive and -resistant visna viruses: isolation and biological comparison.

    abstract::The plaques formed by wild-type (WT) populations of strain K485 visna (V-K485) virus, strain K796 visna (V-K796) virus, and of progressive pneumonia virus in sheep choroid plexus (SCP) cell cultures are heterogeneous in size. Plaque purification procedures showed that this heterogeneity was due to the presence of two ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Trowbridge RS,Lehmann J,Torchio C,Brophy P

    更新日期:1981-01-01 00:00:00

  • Molecular characteristics of a novel strain of canine minute virus associated with hepatitis in a dog.

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    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Choi JW,Jung JY,Lee JI,Lee KK,Oem JK

    更新日期:2016-08-01 00:00:00

  • Using RRT-PCR analysis and virus isolation to determine the prevalence of avian influenza virus infections in ducks at Minto Flats State Game Refuge, Alaska, during August 2005.

    abstract::This study describes surveillance for avian influenza viruses (AIV) in the Minto Flats State Game Refuge, high-density waterfowl breeding grounds in Alaska. Five hundred paired cloacal samples from dabbling ducks (Northern Pintail, Mallard, Green Wing Teal, and Widgeon) were placed into ethanol and viral transport med...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Runstadler JA,Happ GM,Slemons RD,Sheng ZM,Gundlach N,Petrula M,Senne D,Nolting J,Evers DL,Modrell A,Huson H,Hills S,Rothe T,Marr T,Taubenberger JK

    更新日期:2007-01-01 00:00:00

  • Virological and serological characterization of SRV-4 infection in cynomolgus macaques.

    abstract::The nature of SRV-4 infection in cynomolgus macaques remains unclear to date. Here, we report the monitoring of 24 cynomolgus monkeys that were naturally infected with SRV-4 for virus isolation, proviral load and antibody. The results indicated that the SRV-4 antibody status was statistically correlated to environment...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zao CL,Ward JA,Tomanek L,Cooke A,Berger R,Armstrong K

    更新日期:2011-11-01 00:00:00

  • Molecular detection of canine bufaviruses in wild canids.

    abstract::Novel protoparvoviruses genetically related to human and non-human primate bufaviruses (BuVs) have been detected recently in respiratory and enteric specimens collected from dogs and cats. In this study, by molecular screening of archival collections of faecal samples from wolves and foxes, we detected BuVs with a rat...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Melegari I,Di Profio F,Palombieri A,Sarchese V,Diakoudi G,Robetto S,Orusa R,Marsilio F,Bányai K,Martella V,Di Martino B

    更新日期:2019-09-01 00:00:00

  • Exacerbation of influenzavirus pneumonia by intranasal administration of surfactant in a mouse model.

    abstract::Although surfactant-secreting type II alveolar cells have been shown to be damaged during influenzavirus pneumonia, little is known about the effects of surfactant replacement therapy. We have developed a mouse influenza model, in which viral infection can be localized to the upper respiratory tract or to both the upp...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Iwasaki T,Tamura S,Kumasaka T,Sato Y,Hasegawa H,Asanuma H,Aizawa S,Yanagihara R,Kurata T

    更新日期:1999-01-01 00:00:00

  • Role of Langerhans cells in the infection of the guinea-pig epidermis with foot-and-mouth disease virus.

    abstract::In guinea-pig infected with foot-and-mouth disease virus (FMDV), Langerhans cells in the foot pads increase in number and show viral antigens 24 hours post-inoculation, preceding appearance of virus in epithelial cells and vesiculation. This observation suggests that Langerhans cells may be engaged in virus transport ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: di Girolamo W,Salas M,Laguens RP

    更新日期:1985-01-01 00:00:00

  • Virulence of a novel reassortant canine H3N2 influenza virus in ferret, dog and mouse models.

    abstract::An outbreak of a canine influenza virus (CIV) H3N2 reassortant derived from pandemic (pdm) H1N1 and CIV H3N2 in companion animals has underscored the urgent need to monitor CIV infections for potential zoonotic transmission of influenza viruses to humans. In this study, we assessed the virulence of a novel CIV H3N2 re...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Lyoo KS,Na W,Yeom M,Jeong DG,Kim CU,Kim JK,Song D

    更新日期:2016-07-01 00:00:00