Abstract：
:The deletion of the thymidine kinase (TK) gene of herpesviruses causes a reduction in their virulence. However, the effects of the TK gene in infectious laryngotracheitis virus (ILTV) have not been clearly elucidated. In the present study, we constructed a TK gene-deleted recombinant ILTV expressing the green fluorescent protein (GFP) gene as a marker. The GFP gene was inserted in place of the TK gene in both virulent and low virulence strains of ILTV. The GFP gene in the recombinants was expressed in chicken kidney cells, LMH cells and in the chorioallantoic membrane of embryonated chicken eggs. The recombinants produced cytopathic effects in chicken kidney cells and LMH cells and formed pocks in the chorioallantoic membrane of embryonated chicken eggs. The growth rate of the recombinant in chicken kidney cells was similar to that of wild type viruses. The recombinants showed a reduction of virulence compared to that of parental viruses and induced protection against virulent ILTV in specific pathogen free chickens. The recombinant expressing GFP gene may be a candidate for a genetically engineered vaccine and provide a means to study growth kinetics and mechanism of latent infection and reactivation of ILTV. In this study, we confirmed that the TK gene is directly related to virulence of ILTV. This is the first report to show the evidence that the TK gene is a major gene related to virulence of ILTV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Han MG,Kweon CH,Mo IP,Kim SJdoi
10.1007/s00705-001-0794-ykeywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
1017-31issue
5eissn
0304-8608issn
1432-8798journal_volume
147pub_type
杂志文章abstract：:HIV-1 genetic diversity information from a pediatric population is scarce. This study enrolled 128 children living with HIV/AIDS, 103 antiretroviral-treated and 25 naive, from the Sao Paulo metropolitan area. Gag, pol and env regions were amplified, and drug resistance mutations, V3 loop, tropism and viral clades were...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0178-7
更新日期：2008-01-01 00:00:00
abstract：:Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuclease activity allo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315620
更新日期：1976-01-01 00:00:00
abstract：:The adsorption and retention properties of native (unfixed) and glutaraldehyde-fixed alfalfa mosaic virus (AMV) antigens to the polystyrene of ELISA plates were studied using [35S]-labelled virus preparations. It was shown that adsorption was a temperature-dependent, relatively slow process which varied between differ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310767
更新日期：1991-01-01 00:00:00
abstract：:The structural proteins of hemorrhagic enteritis (HEV), a turkey adenovirus, were analyzed by polyacrylamide gel electrophoresis (PAGE) and Western blotting using polyspecific, monospecific and monoclonal antibodies for detection. In purified HEV preparations, eleven polypeptides with apparent molecular weights rangin...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309695
更新日期：1992-01-01 00:00:00
abstract：:The first sporadic cases of Fujian/411/02-lineage viruses were recorded in Finland in winter 2001-2002. The first protracted but low-intensity outbreak occurred here during the first half of 2003, and the second outbreak early in autumn 2003, after detection of sporadic influenza A cases in the summer. The calculated ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0635-5
更新日期：2006-02-01 00:00:00
abstract：:To evaluate the impact of new antiretroviral combinations (HAART: Highly Active Anti Retroviral Therapy) on HCV replication and liver enzyme levels, we analysed the changes in HCV viremia and aminotransferase levels in HIV and HCV co-infected patients. Moreover, to evaluate the influence of HCV infection on the effica...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050685
更新日期：2000-01-01 00:00:00
abstract：:Stem-pitting (SP) is the main type of citrus tristeza virus (CTV) that causes severe damage to citrus trees, especially those of sweet orange, in Hunan province, China. Understanding the local CTV population structure should provide clues for effective mild strain cross-protection (MSCP) of the SP strain of CTV. In th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3089-z
更新日期：2017-02-01 00:00:00
abstract：:The gene coding for the nucleocapsid protein NP of the influenza A virus recombinant strain 413 1,1 was characterized biochemically by molecular hybridization and fingerprint analysis. The data presented suggest that this NP gene has evolved by intracistronic recombination between NP genes of virus N and the fowl plag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322701
更新日期：1980-01-01 00:00:00
abstract：:At least 28 polypeptides have been identified in intracellular virus, with molecular weights ranging from 11,500 to 243,000 daltons. By treatment with Nonidet P-40 and 2-mercaptoethanol it is possible to obtain subviral particles that have lost some proteins and have a density in CsCl of 1.31 g/cm3 which is higher tha...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314979
更新日期：1980-01-01 00:00:00
abstract：:A hemagglutinating virus (8KS0813) was isolated from a red-necked stint. Hemagglutination inhibition and neutralization tests indicated that 8KS0813 was antigenically related to a prototype strain, APMV-6/duck/Hong Kong/18/199/77, but with an 8- and 16-fold difference, respectively, in their titers. The full genome se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2162-8
更新日期：2014-11-01 00:00:00
abstract：:An attempt has been made to devise computer software that will aid virologists to identify unknown virus isolates using the World Wide Web. Computerized information from the Animal Virus Information System was used to obtain data on various characters of a virus species. Sequence data banks are used to obtain the mole...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050394
更新日期：1998-01-01 00:00:00
abstract：:Peritoneal exudate cells (PEC) of DBA/2 mice, after 7 days of in vitro preculture and consisting of virtually 100 per cent macrophages, were able to support the replication of Herpes Simplex Virus type 1 strain WAL (HSV). Using a standard medium based on Dulbecco's Modified Eagle Medium (D-MEM), no virus replication w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309370
更新日期：1984-01-01 00:00:00
abstract：:Six isolates of Cassava brown streak virus (CBSV, genus Ipomovirus; Potyviridae) from the Lake Victoria basin in Uganda and Tanzania were characterized. Virus particles were 650 nm long. The complete coat protein (CP)-encoding sequences (1,101 nucleotides, nt) were 90.7-99.5 and 93.7-99.5% identical at the nt and amin...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0301-9
更新日期：2009-01-01 00:00:00
abstract：:A virus isolated from Olea europaea L. grown in Portugal, was identified as a member of the species Tobacco necrosis virus D (TNV-D, genus Necrovirus, family Tombusviridae), based on the molecular and serological properties of the purified virus particles. The genomic region encoding the coat protein (CP) of this isol...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0258-7
更新日期：2004-06-01 00:00:00
abstract：:Between 2005 and 2011, 23 lineages of circulating vaccine-derived polioviruses (cVDPVs) were detected in Nigeria with nonstructural region (NSR) of non-polio enterovirus C (NPEV-C) origin. However, no information exists on NPEV-C strains recombining with oral poliovirus type 2 vaccine strains (OPV2) to make type 2 cVD...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2322-x
更新日期：2015-03-01 00:00:00
abstract：:We analyzed retrospectively a relative risk of measles attacks in vaccinated vs. unvaccinated students using two independent outbreaks in Japan. The first involved 33/328 (10%) students where 64% students and 30% measles cases had been vaccinated. The second involved 27/241 (11%) students where 81% students and 48% me...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309868
更新日期：1995-01-01 00:00:00
abstract：:While infectious bursal disease virus (IBDV) mainly targets immature B cells and causes T cell infiltration in the bursa of Fabricius (BF) of chickens, the effect of IBDV infection on the properties of T cells and relevant cytokine production in avian gut-associated lymphoid tissues (GALTs) remains unknown. Here, we s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04735-y
更新日期：2020-10-01 00:00:00
abstract：:A simultaneous decay of the expression of Im 140 kDa, Im 150 kDa and Im 160 kDa high MW membrane antigens, concomitant with the cell proliferation arrest, was observed during erythropoietin induced differentiation of ts 34 AEV-transformed erythroid cells cultivated at the restrictive temperature. Expression of embryo-...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310975
更新日期：1987-01-01 00:00:00
abstract：:The complement fixing antigens of rubella virus have been found to contain virion envelope but little nucleocapsid polypeptides. They are also shown to be associated with two additional polypeptides of host origin. Evidence is presented to suggest that the complement fixing antigens are structural components of the pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315301
更新日期：1986-01-01 00:00:00
abstract：:Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of duplicated TK DNA se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01378643
更新日期：1993-01-01 00:00:00
abstract：:A replication-defective, recombinant Sindbis virus vector was utilized in a novel immunization strategy to induce humoral and cellular responses against hepatitis C virus (HCV). The recombinant vector, pVaXJ-E1E2, expressing the gene for HCV glycoproteins E2 and E1, was constructed by inserting the E1E2 gene into the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1564-8
更新日期：2013-05-01 00:00:00
abstract：:Here, we report a novel member of the genus Polerovirus, zucchini aphid-borne yellows virus (ZABYV), which was identified in zucchini grown for seed production in the Xinjiang Uygur Autonomous Region, China. The complete nucleotide sequence of the ZABYV genome was determined and found to be 5,792 nucleotides in length...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04217-w
更新日期：2019-08-01 00:00:00
abstract：:The amount of viroid RNA required for sequence analysis can be reduced by five to six orders of magnitude when a modified "polymerase chain reaction" (PCR) is used for the amplification of the reversely transcribed, overlapping viroid cDNAs. By applying this procedure it is possible to establish the molecular structur...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313962
更新日期：1989-01-01 00:00:00
abstract：:Morbillivirus infection of marine mammals has been documented across all of the world's oceans. Whilst infection is generally demonstrated using a variety of histopathological and serological techniques, where possible, the use of molecular techniques is being used to enable accurate genetic typing of virus strains th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1042-8
更新日期：2011-10-01 00:00:00
abstract：:Genetic analysis of the complete haemagglutinin (HA) gene of fourteen Nigerian avian influenza isolates showed multiple basic amino acids at the cleavage site (321PQRERRRK del R*GLF333), characteristic of highly pathogenic avian influenza (HPAI). Substitution of Gln to Lys at position 322 (H5-specific numbering) was i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3149-4
更新日期：2017-03-01 00:00:00
abstract：:The sequence of RNA genome segment S4 of the avian reovirus (ARV) strain S1133 was determined. S4 RNA is 1185 base pairs long and contains one open reading frame encoding a protein of 367 amino acid residues (40.6 kDa), the similar size as the known S4 gene product (sigma NS), with a net charge of -1 at neutral pH. Th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050258
更新日期：1997-01-01 00:00:00
abstract：:Senecavirus A (SVA) is associated with vesicular disease in swine and the acute death of neonatal piglets. Here, senecavirus A was isolated from a pig displaying vesicular disease in Northeast China. The virus was designated as SVA/HLJ/CHA/2016 and its full-length nucleotide sequence was determined and analyzed in com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3480-4
更新日期：2017-10-01 00:00:00
abstract：:Non-fatal rabies was successfully reproduced in rabbits infected intracerebrally with a highly pathogenic strain of street virus isolated from a man who had died of hydrophobia abter a dog bite and in white rats infected intracerebrally with the CVS strain of fixed virus. All the animals were pretreated with a subleth...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318240
更新日期：1975-01-01 00:00:00
abstract：:Recently we have described that the Herpes simplex virus (HSV)-induced thymidine kinase (TK) induces AMP- and ADP-dThd-5'-phosphotransferase activities. We now demonstrate the heterogeneity of the described activities in isoelectric focusing experiments and polyacrylamide gel electrophoresis. A TK--mutant of HSV type ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314875
更新日期：1982-01-01 00:00:00
abstract：:Mice are protected against lethal intraperitoneal and intravenous infection by encephalomyocarditis virus and Semliki Forest virus by sequential treatment with poly I followed by either polyC or poly5-hydroxyC without production of interferon when the treatments are 4 or more hours apart and by the intraperitoneal or ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318024
更新日期：1976-01-01 00:00:00
