Frequent detection and characterization of hepatitis E virus variants in wild rats (Rattus rattus) in Indonesia.

Abstract:

:One hundred sixteen rats (Rattus rattus) captured in Indonesia from 2011 to 2012 were investigated for the prevalence of hepatitis E virus (HEV)-specific antibodies and HEV RNA. Using an ELISA based on HEV genotype 4 with an ad hoc cutoff value of 0.500, 18.1 % of the rats tested positive for anti-HEV IgG. By nested RT-PCR, 14.7 % of the rats had rat HEV RNA, and none were positive for HEV genotype 1-4. A high HEV prevalence among rats was associated with lower sanitary conditions in areas with a high population density. Sixteen of the 17 HEV isolates obtained from infected rats showed >93.0 % nucleotide sequence identity within the 840-nucleotide ORF1-ORF2 sequence and were most closely related to a Vietnamese strain (85.9-87.9 % identity), while the remaining isolate differed from known rat HEV strains by 18.8-23.3 % and may belong to a novel lineage of rat HEV. These results suggest a wide distribution of rat HEV with divergent genomes.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Mulyanto,Depamede SN,Sriasih M,Takahashi M,Nagashima S,Jirintai S,Nishizawa T,Okamoto H

doi

10.1007/s00705-012-1462-0

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

87-96

issue

1

eissn

0304-8608

issn

1432-8798

journal_volume

158

pub_type

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