Abstract:
:A plaque assay was developed for feline parvovirus (FPV; feline panleucopaenia virus) in a feline embryo (FEmb) cell line. Higher numbers and larger diameter plaques were obtained with a) seeding rates of 0.7 X 10(5) and 1.5 X 10(5) cells cf. 3 X 10(5) and 6 X 10(5) cells/well of 35 mm diameter, b) synchronised cells infected at the G1-S interface cf. nonsynchronised cells and c) 5 to 6 days incubation post inoculation. The plaque assay was standardised by using serum deprivation for 24 hours to synchronize cells, a seeding rate of 1.5 X 10(5) cells/35 mm diameter well, inoculation of virus 16 hours post seeding followed by 5 days incubation. The standardised assay gave consistent, reproducible results. A dose-response curve using the assay showed a linear, 45 degrees slope, relationship between plaque forming units and virus dilution which further verified the sensitivity and reliability of the assay. Plaques produced by "wild" type and plaque purified virus were invariably non uniform in diameter; diameter of plaques in fact followed a normal frequency distribution under standard assay conditions.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Tham KM,Studdert MJdoi
10.1007/BF01378978subject
Has Abstractpub_date
1985-01-01 00:00:00pages
261-8issue
3-4eissn
0304-8608issn
1432-8798journal_volume
84pub_type
杂志文章abstract::Herpes simplex virus type 1 (HSV-1) infection in mutant mice with severe combined immunodeficiency (SCID mice), i.e., mice in which the differentiation of both T and B lymphocytes is severely impaired, was studied. All control (infected and not treated with antibodies or with immune spleen cells) SCID mice were dead b...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01319810
更新日期:1988-01-01 00:00:00
abstract::Simian varicella virus (SVV) infection of non-human primates is used as a model to study the pathogenesis and latency of varicella-zoster virus (VZV), the etiological agent of chickenpox and shingles. Uracil DNA glycosylase (UDG) is a DNA repair enzyme responsible for excision of uracil residues misincorporated into D...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050630
更新日期:1999-01-01 00:00:00
abstract::Infectivity of non-occluded particles of nuclear polyhedrosis virus infecting Galleria mellonella L. was determined with bioassay technique in vivo and with plaque assay method in vitro using SCLd 135 (Quiot) established cell culture. A plaque-forming virus unit corresponds to about 50 ID50 for Galleria larvae injecte...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315023
更新日期:1979-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314603
更新日期:1981-01-01 00:00:00
abstract::Four geographic variants (topotypes) of yellow fever (YF) virus from Africa and South America previously determined by RNA oligonucleotide mapping were analyzed for their structural, antigenic and virulence characteristics. The electrophoretic migration mobility and carbohydrate content of the envelope protein E chara...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310727
更新日期:1987-01-01 00:00:00
abstract::Structural analysis of the US-segment of a viable temperature sensitive human cytomegalovirus mutant (ts9) by the use of restriction enzymes, specific amplifications by the polymerase chain reaction and DNA sequencing, revealed deletions of open reading frames (orf) US14 and part of US15 in addition to that concerning...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311183
更新日期:1994-01-01 00:00:00
abstract::A new bisegmented dsRNA virus has been detected in shoots of blue spruce with bud blight disease symptoms and infection by Cucurbitaria piceae (Bortw.) fungus. The virus genome consists of two segments that are 2071 and 2257 nt long, encoding the putative RNA polymerase and capsid protein, respectively. Rosellinia nec...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2692-8
更新日期:2016-05-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310981
更新日期:1987-01-01 00:00:00
abstract::Begomovirus infection is becoming a threat in fresh-market tomato in Argentina, where mixed infections with begomoviruses are common. The complete sequence of a begomovirus isolate infecting tomato sampled in Salta was molecularly characterized. Phylogenetic analysis showed that this virus isolate is closely related t...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1355-2
更新日期:2012-10-01 00:00:00
abstract::The susceptibility of mice of different ages (from four to 28 days) to infection with herpes simplex virus type 1 (HSV-1) mutants inoculated onto scarified corneas was studied. The TK+ isolate from wild type virus was pathogenic in mice of all age groups. An HSV-1 mutant (designated TK1/4) with a less active thymidine...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311326
更新日期:1983-01-01 00:00:00
abstract::Monitoring of influenza A virus (IAV) was conducted in wild bird species in central Thailand. Four IAV subtype H12N1 strains were isolated from a watercock (order Gruiformes, family Rallidae) (n = 1) and lesser whistling ducks (order Anseriformes, family Anatidae) (n = 3). All H12N1 viruses were characterized by whole...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1260-8
更新日期:2012-06-01 00:00:00
abstract::Hepatitis B virus (HBV) circulates as a collection of genetically related variants that evolve throughout the chronic infection. Those viral variants that have the greatest fitness are fixed. We recently showed different fitness for HBV variants involved in two epidemiological situations. To understand these fitness d...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-4090-5
更新日期:2019-02-01 00:00:00
abstract::The complete genome of ϕ16, a temperate corynephage from Corynebacterium glutamicum ATCC 21792, was sequenced and annotated (GenBank: KY250482). The electron microscopy study of ϕ16 virion confirmed that it belongs to the family Siphoviridae. The ϕ16 genome consists of a linear double-stranded DNA molecule of 58,200 b...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3383-4
更新日期:2017-08-01 00:00:00
abstract::Bovine leukemia virus (BLV) causes enzootic bovine leucosis (EBL) and is responsible for substantial economic losses in cattle globally. However, information in Africa on the disease is limited. Here, based on clinical, hematological, pathological and molecular analyses, two clinical cases of EBL were confirmed in a d...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3205-0
更新日期:2017-04-01 00:00:00
abstract::During aetiological study of diseased red clover (Trifolium pratense L.) using high throughput sequencing, a novel virus with a 10 kb genome divided into two segments was discovered. The virus, tentatively named red clover associated varicosavirus (RCaVV), is phylogenetically related to classifiable members of the gen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3838-2
更新日期:2018-08-01 00:00:00
abstract::Heparin added together with murine L cell interferon inhibited the development of antiviral activity in mouse L cells. When added after interferon treatment, heparin had no effect on antiviral activity. There was also no inhibition of interferon action in L cells treated with heparin before addition of interferon. On ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314146
更新日期:1986-01-01 00:00:00
abstract::Use of Urografin and Conray for the equilibrium centrifugation of viruses is described. These pharmaceuticals, which consist of iodinated arylic compounds, reach densities of 1.6 g/cm3 and have low intrinsic viscosities. Poliovirus, Newcastle disease virus, and lymphocytic choriomeningitis virus were centrifuged to eq...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318245
更新日期:1975-01-01 00:00:00
abstract::Torque teno sus virus 1 (TTSuV1) is a novel virus that has been found widely distributed in the swine population in recent years. Analysis of codon usage can reveal much about the molecular evolution of TTSuV1. In this study, synonymous codon usage patterns and the key determinants in the coding region of 29 available...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1480-y
更新日期:2013-01-01 00:00:00
abstract::The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an int...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718322
更新日期:1996-01-01 00:00:00
abstract::The genomes of all replication-competent retroviruses contain cis-acting elements that regulate gene expression. However, the identities of many of these elements remain to be characterized. Inserting sequences into the 3' untranslated region of a replication-competent spleen necrosis virus (SNV) vector disrupted its ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050486
更新日期:1999-01-01 00:00:00
abstract::Grapevine Pinot gris virus (GPGV), a member of the genus Trichovirus in the family Betaflexiviridae, was recently discovered in Italy and subsequently in other European countries and in Korea. In this study, we assessed the occurrence of GPGV in 441 samples from Western and Eastern Europe collected over the period 200...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2718-2
更新日期:2016-03-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2810-2
更新日期:2016-06-01 00:00:00
abstract::We have sequenced the complete genome of an isolate of Banana streak virus from banana cv. 'Mysore' and show that it is sufficiently different from a previously characterised isolate from cv. 'Obino l'Ewai' to warrant recognition as a distinct species, for which the name Banana streak Mysore virus (BSMysV) is proposed...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0471-z
更新日期:2005-04-01 00:00:00
abstract::Genetic relationships among echovirus type 25 (ECV 25) isolates associated with aseptic meningitis in Germany 1997/98 and a 40-year-old ECV 25 prototype strain were investigated using RT-PCR and direct sequencing the VP1/2A gene junction region. Sequences were compared to each other and to non-polio enterovirus repres...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050640
更新日期:1999-01-01 00:00:00
abstract::Adenoviruses are common pathogens that are responsible for a wide variety of infectious syndromes. The objectives of this study were to identify and characterize members of different adenovirus species at the molecular level and to describe the correlation between viruses and clinical syndromes during a period of 4 ye...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0338-4
更新日期:2009-01-01 00:00:00
abstract::In this study, we calculated the relative synonymous codon usage (RSCU) value and the effective number of codons (ENC) value to carry out principal component analysis (PCA) and correlation analysis of the codon usage pattern of the phosphoprotein gene (P gene) of spring viraemia of carp virus (SVCV). The synonymous co...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2000-z
更新日期:2014-07-01 00:00:00
abstract::Systemic infection of tobacco with tobacco mosaic virus (TMV) strain WU1, is accompanied by massive accumulation of the virus-coded non-structural 126 kDa protein in X-bodies. The development of X-bodies and the time course of the increase in 126 kDa protein in systemically infected leaves were analyzed by immunocytoc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309584
更新日期:1992-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3260-1
更新日期:2017-07-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0265-9
更新日期:2009-01-01 00:00:00
abstract::The ICP34.5 gene from HSV-2 strain 333 was cloned and, when expressed in Vero cells, enhanced the efficiency and extent of glycoprotein processing of glycoprotein C (gC1), a representative viral glycoprotein, during infection with HSV-1 SP7. The ICP34.5 from HSV-1 SP7 limits the extent and efficiency of viral glycopro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0341-9
更新日期:2009-01-01 00:00:00