Abstract：
:Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, human microvascular endothelial cells (HMEC-1) infected with a DENV-2 clinical isolate, or HMEC-1 cells transfected with its protease sequence (NS3pro) or its complex (NS2BNS3pro) were able to trigger apoptosis after 24 h of infection or transfection. The infected or transfected HMEC-1 cells displayed the distinctive apoptotic hallmarks, which include cytoplasmic shrinkage and plasma membrane blebbing. In addition, the transfected HMEC-1 cells showed biochemical changes such as exposure of phosphatidylserine on the outer leaflet of the plasma membrane, TUNEL positivity, caspase 3 activation and cleaved PARP, a central regulator of apoptosis. These findings suggest the role of such proteins from the clinical isolate in the induction of apoptosis.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Vásquez Ochoa M,García Cordero J,Gutiérrez Castañeda B,Santos Argumedo L,Villegas Sepúlveda N,Cedillo Barrón Ldoi
10.1007/s00705-009-0396-7subject
Has Abstractpub_date
2009-01-01 00:00:00pages
919-28issue
6eissn
0304-8608issn
1432-8798journal_volume
154pub_type
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