Exacerbation of influenzavirus pneumonia by intranasal administration of surfactant in a mouse model.

Abstract:

:Although surfactant-secreting type II alveolar cells have been shown to be damaged during influenzavirus pneumonia, little is known about the effects of surfactant replacement therapy. We have developed a mouse influenza model, in which viral infection can be localized to the upper respiratory tract or to both the upper and lower respiratory tract depending on the volume (rather than infectious dose) of intranasal inocula of influenzavirus. In this model, only mice infected with a large inocula die with massive infection in the lung. Using this model, we unexpectedly found that intranasal administration of surfactant dramatically exacerbated influenzavirus infection causing fatal disease even in mice inoculated with a small inocula. This exacerbation resulted from enhancement of intrabronchial and intraalveolar spread of virus, as confirmed by immunohistochemical detection of viral antigen in lungs. Assuming this experimental model in mice recapitulates naturally occurring disease in humans, extreme caution is warranted in surfactant-replacement treatment of influenzavirus pneumonia in humans.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Iwasaki T,Tamura S,Kumasaka T,Sato Y,Hasegawa H,Asanuma H,Aizawa S,Yanagihara R,Kurata T

doi

10.1007/s007050050535

keywords:

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

675-85

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

144

pub_type

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