Abstract:
:Although cell-mediated cytotoxicity effectively kills target virus-infected cells, no careful consideration has been given to the fate of infectious progeny virus contained within target cells following the cytolytic event. To address this issue with respect to murine cytomegalovirus (MCMV) pathogenesis, we developed a rapid semiquantitative assay for infectious MCMV based on expression of beta-galactosidase using a LacZ-expressing recombinant MCMV. Simultaneous use of this assay in combination with a modified cell-mediated cytotoxicity assay revealed that cytotoxicity of MCMV-infected target cells mediated by MCMV-immune splenic cells does not lead to inactivation of intracellular infectious virus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Dix RD,Cousins SWdoi
10.1007/s00705-004-0459-8keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
797-803issue
4eissn
0304-8608issn
1432-8798journal_volume
150pub_type
杂志文章abstract::To characterize the role of the humoral immune response on HIV-1 infection of monocytes and macrophages (M phi s) we examined the susceptibility of in vitro cultured monocyte/M phi s to various HIV-1 isolates and the influence of heterologous and particularly autologous anti HIV-1 sera on this infection. Depending on ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01538815
更新日期:1994-01-01 00:00:00
abstract::Analysis of the preS1 gene of hepatitis B virus was used to define two nosocomial outbreaks of HBV infection. In an outbreak in an oncology unit we had previously shown by single stranded conformational polymorphism (SSCP) analysis of a 189 bp fragment of the preS1 gene, that 52 children were infected with HBV strains...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050200
更新日期:1997-01-01 00:00:00
abstract::The molecular epidemiology of a recent norovirus (NoV) outbreak in Brazil performed by comparative analysis with Genebank NoV sequences showed that the GII.4 strain was responsible for 72.5% of all NoV-positive cases (58/80). Other detected NoV strains included GII.3 (7/80; 8.8%) and GII.9 (8/80; 10%). This is the fir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0078-x
更新日期:2008-01-01 00:00:00
abstract::To eradicate rabies in foxes, almost 97 million oral rabies vaccine baits have been distributed in Germany and Austria since 1983 and 1986, respectively. Since 2007, no terrestrial cases have been reported in either country. The most widely used oral rabies vaccine viruses in these countries were SAD (Street Alabama D...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0408-7
更新日期:2009-01-01 00:00:00
abstract::Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny vir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314121
更新日期:1985-01-01 00:00:00
abstract::Virazole (1, beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide = Ribavirin), a synthetic nucleoside analogue, has been successfully used against tomato spotted wilt virus (TSWV) in tomato and tobacco plants. In tomato, the most efficient concentration to control TSWW was 500 mg/l while in tobacco, concentrations of 10...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315037
更新日期:1980-01-01 00:00:00
abstract::This study examines viral protein and DNA synthesis in Spodoptera frugiperda multicapsid nuclear polyhedrosis virus (SfMNPV) infections of S. frugiperda and Trichoplusia ni cells. A total of 28 infected cell specific polypeptides (ICSPs) were detected in the productive S. frugiperda cells. Of these, 14 were identified...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310626
更新日期:1990-01-01 00:00:00
abstract::Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this populati...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3224-5
更新日期:2017-06-01 00:00:00
abstract::A xenotropic Moloney murine leukemia virus-derived recombinant retrovirus (MMuLVSVnlslacZ) has been utilized to study the mechanism of virus entry into endothelial and epithelial porcine cells. In the genome of this recombinant retrovirus, the nlslacZ reporter gene is under the transcriptional control of both LTR and ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314625
更新日期:1992-01-01 00:00:00
abstract::A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313815
更新日期:1989-01-01 00:00:00
abstract::Hepatitis C virus (HCV) genome contains a 3'noncoding region (3'NCR) consisting of a variable region, a polypyrimidine tract (polyU/UC) and the X region. To examine the roles of 3'NCR and polyU/UC tract in the internal ribosome entry site (IRES)-mediated translation process, a variety of 3'NCRs containing different le...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0491-3
更新日期:2005-06-01 00:00:00
abstract::Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously dete...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2217-x
更新日期:2015-01-01 00:00:00
abstract::Infectious bursal disease virus (IBDV), a member of the family Birnaviridae, is a non-enveloped, double-stranded RNA virus. Viral protein 1 (VP1), the putative RNA-dependent RNA polymerase, occurs in virions both as a free polypeptide and as a genome-linked protein, called VPg. To gain more insight in its function, we...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0365-0
更新日期:2004-11-01 00:00:00
abstract::The genomic RNA of infectious flacherie virus (IFV), an insect picornavirus, was shown to contain a covalently attached substance which can be labeled with 125I-Bolton and Hunter reagent. This substance was found to be a protein covalently linked to the virus genome, because it cosedimented and was copurified with the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317378
更新日期:1986-01-01 00:00:00
abstract::Drosophila C virus RNA acted as mRNA in rabbit reticulocyte lysates and directed the synthesis of at least one capsid protein and a number of higher molecular weight proteins. Kinetic analysis by pulse-chase experiments showed that a number of high molecular weight products acted as precursors to the capsid protein(s)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311694
更新日期:1983-01-01 00:00:00
abstract::The aim of this study was to develop and evaluate a two-tube multiplex real-time RT-PCR assay for the detection and identification of four viral hemorrhagic fever (VHF) pathogens, severe fever with thrombocytopenia syndrome virus (SFTSV), Hantaan virus (HTNV), Seoul virus (SEOV), and dengue virus (DENV), from human cl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1677-8
更新日期:2013-09-01 00:00:00
abstract::A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respe...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3196-x
更新日期:2017-04-01 00:00:00
abstract::We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2024-4
更新日期:2014-08-01 00:00:00
abstract::Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HER...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0764-5
更新日期:2006-10-01 00:00:00
abstract::Anti-human T-lymphotropic virus type I/II (HTLV-I/II) antibodies were screened by particle agglutination test in a total of 66 patients with thalassemia major who received multiple transfusion from paid donors at the Blood Transfusion Hematology Center of Ho Chi Minh City in South Vietnam. HTLV-II infection was confir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050170
更新日期:1997-07-01 00:00:00
abstract::Work from a number of laboratories including our own has shown that foreign B-epitopes inserted into the c/e1-region of Hepatitis B core antigen (HBcAg) elicit powerful antibody responses when mice are immunised with the recombinant core particles. In the present study, we wished to take advantage of the immunodominan...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050590
更新日期:1999-01-01 00:00:00
abstract::Six synthetic peptides corresponding to regions of bovine enterovirus (BEV), strain VG-5-27, elicited antibodies in mice which reacted with the virus in various assays. These antibodies have been characterised on the basis of their ability to (1) neutralize the virus, (2) bind to the intact virus particle in an immuno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309681
更新日期:1992-01-01 00:00:00
abstract::A collection of rotavirus samples collected over a 26-year period was examined to study the dynamics of change in RV strains of genotype P[8] in a geographically defined population (Nizhny Novgorod, Russia; children under 6 years) with no vaccine pressure. Phylogenetic analysis of gene VP4 (subunit VP8*) showed the pr...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1426-4
更新日期:2012-12-01 00:00:00
abstract::The basic structure of human immunodeficiency virus type 1 (HIV-1) has been investigated morphologically; however, the internal structure of HIV-1 core is not well understood. We studied the internal structures by transmission electron microscopy. We modified the method for electron staining of ultrathin sections and ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050083
更新日期:1997-01-01 00:00:00
abstract::Tetracyclines have been used to treat many bacterial infections. The use of these antibiotics for the treatment of viral diseases dates to the 1960s to 1970s. Over the decades, the effect of tetracyclines on the pathogenesis of viral infections has been demonstrated both clinically and experimentally. Tetracyclines ca...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-020-04860-8
更新日期:2021-01-01 00:00:00
abstract::The four CC chemokine-like proteins (Fpv060, Fpv061, Fpv116 and Fpv121) of fowlpox virus (FWPV) were over-expressed as His-tagged versions from a T7 promoter/EMCV IRES construct in vitro, by coupled transcription/translation, or in cell culture, by co-infection with two recombinant FWPVs (one expressing the chemokine-...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0560-7
更新日期:2005-09-01 00:00:00
abstract::We previously reported the isolation of South River virus (SORV) from a pool of mosquitoes collected in the Yucatan Peninsula of Mexico (Farfan-Ale et al. in Vector Borne Zoonotic Dis 10:777-783, 5). The isolate (designated SORV-252) was identified as SORV after a 197-nucleotide region of its small RNA genome segment ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1280-4
更新日期:2012-06-01 00:00:00
abstract::Previously, we identified serological immunodeterminants of African swine fever virus (ASFV), including pK205R and pB602L, without homologues in the database. pK205R is expressed as a 33-kD protein from 4 h post-infection onward, initially diffusely distributed throughout cells, and subsequently in viral factories. pK...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0246-z
更新日期:2008-01-01 00:00:00
abstract::This study reports the molecular epidemiology and genetic characterization of human respiratory syncytial virus (RSV) samples collected in Thailand from January 2010 to December 2011. In total, 1,315 clinical samples were collected from Bangkok and Khon Kaen provinces and were screened by semi-nested PCR for RSV infec...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1773-9
更新日期:2014-03-01 00:00:00
abstract::Scutellaria baicalensis Georgi, a Chinese herbal decoction, has been used for the treatment of the common cold, fever and influenza virus infections. In previous studies, we found that oral administration of baicalein resulted in the inhibition of influenza A virus replication in vivo, which was linked to baicalin in ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2192-2
更新日期:2014-12-01 00:00:00