Abstract:
:Work from a number of laboratories including our own has shown that foreign B-epitopes inserted into the c/e1-region of Hepatitis B core antigen (HBcAg) elicit powerful antibody responses when mice are immunised with the recombinant core particles. In the present study, we wished to take advantage of the immunodominance of the c/e1-region to deliver cytotoxic T-lymphocyte (CTL) epitopes as a recombinant HBcAg vaccine. Our results indicated that recombinant HBcAg containing CTL epitopes of the E7 protein of human papillomavirus failed to prime E7-directed CTL responses when used to immunise mice for antigen processing through either the endogenous pathway via a Salmonella typhimurium vector, or through the exogenous pathway by parenteral immunisation with recombinant core. Hydropathicity plots predict that the presumed surface location of the hydrophilic c/e1-region within the core particle may alter following insertion of hydrophobic residues constituting the CTL epitopes, thereby compromising their presentation to the afferent immune system. Our data indicate that while the c/e1-region has a powerful adjuvanting effect for inserted B-epitopes, it does not serve this function for inserted CTL epitopes. These findings have generic implications for the development of CTL inducing vaccines using HBcAg as a vaccine vehicle.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Street M,Herd K,Londono P,Doan T,Dougan G,Kast WM,Tindle RWdoi
10.1007/s007050050590keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
1323-43issue
7eissn
0304-8608issn
1432-8798journal_volume
144pub_type
杂志文章abstract::Resistance to antiretroviral agents is a significant concern in the clinical management of HIV-infected individuals, particularly in areas of the world where treatment options are limited. In this study, we aimed to identify HIV drug-resistance-associated mutations in 40 drug-naïve patients and 62 patients under antir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2955-z
更新日期:2016-09-01 00:00:00
abstract::We previously reported the isolation of South River virus (SORV) from a pool of mosquitoes collected in the Yucatan Peninsula of Mexico (Farfan-Ale et al. in Vector Borne Zoonotic Dis 10:777-783, 5). The isolate (designated SORV-252) was identified as SORV after a 197-nucleotide region of its small RNA genome segment ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1280-4
更新日期:2012-06-01 00:00:00
abstract::We determined the nucleotide sequence of an 11059 bp fragment of the pseudorabies virus genome located in the right part of genomic BamHI fragment 3 and the adjacent part of BamHI fragment 6. Within this region eight open reading frames were identified whose deduced amino acid sequences exhibited homology to the UL6, ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050056
更新日期:1997-01-01 00:00:00
abstract::A full length cDNA copy of the genomic RNA of lettuce mosaic virus (LMV) was constructed under the control of an enhanced CaMV 35S promoter and of the NOS terminator. This construct was found infectious when inoculated to lettuce plants. The intron II of the bean nitrite reductase gene was engineered into the LMV FL c...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050474
更新日期:1998-01-01 00:00:00
abstract::Norovirus has captured increasing attention as an agent of childhood diarrhoea. However, it is not known whether norovirus causes as severe diarrhoea as rotavirus, particularly among children in developing countries. In a 1-year study conducted between May 2004 and April 2005 in Recife, Brazil, norovirus was detected ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0060-7
更新日期:2008-01-01 00:00:00
abstract::Multiple splicing sites exist in the RNA genome of the human immunodeficiency virus type 1 (HIV-1). In a screen for subgenomic forms of the HIV-1 genome that could be transferred to fresh cells by virus infection, we identified a novel spliced variant of HIV-1 RNA that uses a hitherto unknown splice acceptor site with...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718159
更新日期:1996-01-01 00:00:00
abstract::Drosophila C virus RNA acted as mRNA in rabbit reticulocyte lysates and directed the synthesis of at least one capsid protein and a number of higher molecular weight proteins. Kinetic analysis by pulse-chase experiments showed that a number of high molecular weight products acted as precursors to the capsid protein(s)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311694
更新日期:1983-01-01 00:00:00
abstract::cDNA clones spanning the entire region of the peplomer (S) gene of feline infectious peritonitis virus (FIPV) type I strain KU-2 were obtained and their complete nucleotide sequences were determined. A long open reading frame (ORF) encoding 1464 amino acid residues was found in the gene, which was 12 residues longer t...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718424
更新日期:1995-01-01 00:00:00
abstract::A small unconventional, viroid-like aetiological agent might initiate Crohn's disease (CD). Electrophoresis of radiolabelled low molecular weight nucleic acids from mesenteric lymph nodes and leucocytes did not distinguish CD-specific sequences compatible with a viroid-like agent. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310895
更新日期:1986-01-01 00:00:00
abstract::Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309576
更新日期:1992-01-01 00:00:00
abstract::Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradeca...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310506
更新日期:1990-01-01 00:00:00
abstract::The bisegmented genome of a putative double-stranded (ds) RNA virus from Ustilaginoidea virens was sequenced and analyzed. The larger genomic segment of 2112 bp encodes a putative RNA-dependent RNA polymerase (RdRp, 628 aa), and the smaller one of 2082 bp encodes a putative coat protein (CP) of 539 aa. The 5' untransl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-1991-9
更新日期:2014-07-01 00:00:00
abstract::The leafhopper Euscelidius variegatus is a natural vector of chrysanthemum yellows phytoplasma (CY) and an efficient vector of flavescence dorée phytoplasma (FD) under laboratory conditions. During a transcriptome sequencing (RNA-seq) project aimed at investigating the interactions between the insect and the two phyto...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3158-3
更新日期:2017-03-01 00:00:00
abstract::We have sequenced the genome of a novel picornavirus, rabovirus A (rat-borne virus, RaBoV-A, NC_026314), which was present in the feces of a Norway rat (Rattus norvegicus) from Berlin, Germany. This virus is related to members of the genera Enterovirus and Sapelovirus. RaboV-A contains a type II IRES that is unlike th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2523-y
更新日期:2015-10-01 00:00:00
abstract::Salmonella phages SenALZ1 and SenASZ3, two novel phages infecting Salmonella enterica, were isolated and analyzed. The genomes of these two phages consist of 154,811 and 157,630 base pairs (bp), with G+C contents of 44.56% and 44.74%, respectively. Fifty-nine of 199 open reading frames (ORFs) in the SenALZ1 genome, an...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04183-3
更新日期:2019-05-01 00:00:00
abstract::A virus associated with yellow-to-purple ringspot on honeysuckle plants has been detected and tentatively named honeysuckle ringspot virus (HnRSV). The complete nucleotide sequence of HnRSV from infected honeysuckle has been determined. The genomic RNA of HnRSV is 3,956 nucleotides in length and is predicted to contai...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1009-9
更新日期:2011-09-01 00:00:00
abstract::BHK monolayer and suspension cell populations maintained in different laboratories were found to vary in their susceptibility to infection with three strains of foot-and-mouth disease virus (FMDV). The susceptibility of parent cell populations was compared with that of individual clones of cells derived from them. The...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320756
更新日期:1980-01-01 00:00:00
abstract::This report presents a molecular characterization of the complete genome of a rare hepatitis C virus (HCV) genotype (GT5a) from India. Sequence homology of full genome revealed that the strain belonged to HCV GT5a. To trace the origin of this virus and to understand its evolutionary pattern, a phylogenetic reconstruct...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2220-2
更新日期:2015-01-01 00:00:00
abstract::We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella. Electron microscopy revealed that vB_ButM_GuL6 belongs to the family Myoviridae, order Caudovirales. The genome of vB_ButM_GuL6 is a linear, circularl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04780-7
更新日期:2020-11-01 00:00:00
abstract::Current subgenotype 2.1 isolates of classical swine fever virus (CSFV) play a dominant role in CSF outbreaks in China, and a novel sub-subgenotype 2.1g of CSFV was recently identified, but the complete genome sequence of this new sub-subgenotype has not been reported. In this study, complete genome of 2.1g isolate GD1...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2932-6
更新日期:2016-09-01 00:00:00
abstract::Noninfectious virus particles were produced in Ehrlich ascites tumor cells infected intraperitoneally with fowl plague virus. The PFU yield of virus per cell was less than 0.1 and the ratio PFU/HA units in the progeny virus was less than 10(3). The virus particles had the same morphology and size as egg-grown virus bu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315278
更新日期:1978-01-01 00:00:00
abstract::Six synthetic peptides corresponding to regions of bovine enterovirus (BEV), strain VG-5-27, elicited antibodies in mice which reacted with the virus in various assays. These antibodies have been characterised on the basis of their ability to (1) neutralize the virus, (2) bind to the intact virus particle in an immuno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309681
更新日期:1992-01-01 00:00:00
abstract::Sixteen patients with symptoms typical for Ockelbo disease (rash, arthralgia, fever) were enrolled in a 2 1/2 year study, during which clinical symptoms were recorded and ELISA was employed to study specific IgM, IgG and IgG subclass development. Initially, all patients presented with rash and arthralgia, and five pat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379107
更新日期:1994-01-01 00:00:00
abstract::Fecal virus shedding, seroconversion and histopathology were evaluated in 3-7-year-old gnotobiotic calves orally inoculated with porcine deltacoronavirus (PDCoV) (9.0-9.6 log10 genomic equivalents [GE] of OH-FD22-P5; n=4) or porcine epidemic diarrhea virus (PEDV) (10.2-12.5 log10 GE of PC21A; n=3). In PDCoV-inoculated...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3351-z
更新日期:2017-08-01 00:00:00
abstract::Nanoviruses and geminiviruses are circular, single stranded DNA viruses that infect many plant species around the world. Nanoviruses and certain geminiviruses that belong to the Begomovirus and Mastrevirus genera are associated with additional circular, single stranded DNA molecules (~ 1-1.4 kb) that encode a replicat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3854-2
更新日期:2018-09-01 00:00:00
abstract::The objective of this study was to test the ability of bovine viral diarrhea virus (BVDV) to infect mice. Two mice each were either mock infected or inoculated with one of three BVDV strains by the intraperitoneal (IP) (n = 8) or intranasal (IN) (n = 8) route. All mice were euthanized at day 7 postinfection (p.i.). No...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2412-4
更新日期:2015-06-01 00:00:00
abstract::The '6K1' protein of the Pinellia isolate of Soybean mosaic virus was cloned into a prokaryotic expression vector and a polyclonal antiserum raised to the expressed fusion protein. In immunogold labeling of thin sections of infected leaves of Pinellia ternata, specific labeling occurred at the cell periphery. This mig...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0972-7
更新日期:2007-01-01 00:00:00
abstract::Newborn WKA rats given a single intraperitoneal injection of MMC treated TARS-1, a rat T cell line producing HTLV-I, were shown to accumulate high titred antibodies specific for HTLV-I. Adult WKA rats rejected TARS-1 transplant with transient appearance of anti-HTLV-I antibodies. However, rats maintained under daily a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314420
更新日期:1987-01-01 00:00:00
abstract::Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. In 2013, a large outbreak was reported on the archipelago of French Polynesia. In this study, we report the detection and molecular characterization of Zika virus for the first time in Chile from an outbreak among the inhabitant...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2695-5
更新日期:2016-03-01 00:00:00
abstract::The duration of infection with rat virus (RV), an autonomous rodent parvovirus, was examined at multiple intervals over 6 months in rats inoculated by the oronasal route at 2 days of age or 4 weeks of age and individually housed after weaning to prevent cross-infection. Infectious virus was recovered by explant cultur...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310765
更新日期:1991-01-01 00:00:00