Abstract：
:The genomic RNA of infectious flacherie virus (IFV), an insect picornavirus, was shown to contain a covalently attached substance which can be labeled with 125I-Bolton and Hunter reagent. This substance was found to be a protein covalently linked to the virus genome, because it cosedimented and was copurified with the viral RNA and was degraded by proteinase K but not by RNases. The molecular weight of the protein was found to be 11,500 in the sodium dodecyl sulfate polyacrylamide gel electrophoresis. This protein apparently corresponds to viral protein genome-linked (VPg) detected in mammalian picornaviruses, because it was not required for the synthesis of viral proteins in a cell-free translation system of a rabbit reticulocyte lysate and because it was not immunochemically related to the virus capsid proteins. The results presented here leave a possibility that insect picornavirus also replicates its RNA by using VPg as a primer as reported for mammalian picornaviruses. These observations on VPg of an insect picornavirus are discussed in the view point of comparative virology.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hashimoto Y,Watanabe A,Kawase Sdoi
10.1007/BF01317378subject
Has Abstractpub_date
1986-01-01 00:00:00pages
301-12issue
3-4eissn
0304-8608issn
1432-8798journal_volume
90pub_type
杂志文章abstract：:In this study, we calculated the relative synonymous codon usage (RSCU) value and the effective number of codons (ENC) value to carry out principal component analysis (PCA) and correlation analysis of the codon usage pattern of the phosphoprotein gene (P gene) of spring viraemia of carp virus (SVCV). The synonymous co...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2000-z
更新日期：2014-07-01 00:00:00
abstract：:A viroid was isolated from symptomless Coleus blumei cultivar (cv) 'Fairway Ruby' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viroid was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing revealed...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718241
更新日期：1996-01-01 00:00:00
abstract：:An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P(late), hydroxylamine mutagenesis was pe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0851-7
更新日期：2007-02-01 00:00:00
abstract：:Six novel subgenotypes (B7, B8, C6, C8, C9, and D6) within three hepatitis B virus (HBV) genotypes (B-D) were recently identified in Indonesia. To further characterize HBV in this country, 18 HBV-viremic samples obtained from blood donors in Nusa Tenggara, Indonesia, were subjected to phylogenetic analysis of an 1.6-k...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0628-x
更新日期：2010-05-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is a member of the genus Deltaretrovirus of the family Retroviridae and cause a chronic lymphosarcoma, which is extensive in cattle. In yaks (Bos grunniens), the distribution, strains and genetic characteristics of BLV have rarely been studied. The aim of our study was to investigate BLV in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3658-9
更新日期：2018-03-01 00:00:00
abstract：:Adenovirus type 7 vaccine strain was engineered to express foreign antigens from both the E3 early promoter in the E3 region and the major late promoter inserted between the E4 region and the right inverted terminal repeat. This multiple expression vector was used to express hepatitis B core antigen (HBcAg), hepatitis...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311300
更新日期：1991-01-01 00:00:00
abstract：:Little is known about the bacteriophage proteins expressed immediately after infection of the host cell. Most of these early proteins are probably involved in bacteriophage-host interactions redirecting the bacterial metabolism to phage production. Interaction analysis of the first 16 phiKMV gene products (gp) identif...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0967-4
更新日期：2007-01-01 00:00:00
abstract：:Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not availa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3806-x
更新日期：2018-07-01 00:00:00
abstract：:About 98% of the DNA sequence of the lymphotropic Aleutian disease parvovirus isolate ADV-SL3 was determined and analysed. The sequence revealed that this isolate was a type-1 ADV strain, supporting that the currently used typing of ADV viruses does not correlate with virulence or pathogenicity. ADV-SL3 had a very hig...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050066
更新日期：1997-01-01 00:00:00
abstract：:Two independent isolates of an unusual type of rotavirus were obtained from over 900 faecal samples obtained from piglets with diarrhoea. Genome profiles, containing eleven segments, were not characteristic of group A electropherotypes and corresponded to an atypical virus. The profiles were, however, distinct from th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309892
更新日期：1986-01-01 00:00:00
abstract：:One host polypeptide (40,000 daltons) synthesized prior to infection is associated with the 250S RNA polymerase structure partially purified by a combination of velocity sedimentation and isopycnic separation. A series of pulse-chase experiments have shown that a 56,000 dalton polypeptide made during the eclipse phase...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317808
更新日期：1975-01-01 00:00:00
abstract：:Oncogene-containing retroviruses are generated by recombination events between viral and cellular sequences, a phenomenon called "oncogene capture". The captured cellular genes, referred to as "v-onc" genes, then acquire new oncogenic properties. We report a novel feline leukemia virus (FeLV), designated "FeLV-AKT", t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3192-1
更新日期：2017-04-01 00:00:00
abstract：:A reversed passive hemagglutination (RPHA) method was developed for the detection of bovine coronavirus in fecal specimens. Sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine coronav...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315291
更新日期：1984-01-01 00:00:00
abstract：:We have amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced a 605-bp fragment covering the variable region of the coat protein gene of fish nodaviruses infecting European sea bass, Dicentrarchus labrax (n = 24), and shi drum, Umbrina cirrosa (n = 2), in the Mediterranean basin. Nine new...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170110
更新日期：2001-01-01 00:00:00
abstract：:In this study, an Escherichia coli virulent phage, SRT8, was isolated from sewage sludge samples collected from Jinan, Shandong Province, China. The genome of phage SRT8 consists of 49,579 bp with 47.83% G+C content. The phage genome contains 84 putative protein-coding genes, and no rRNA or tRNA genes. Comparative gen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3778-x
更新日期：2018-06-01 00:00:00
abstract：:Conditions for ELISA by using IgG linked with horseradish peroxidase have been developed for the detection of 6 arenaviruses (Machupo, Junin, Tacaribe, Amapari, LCM and Tamiami). This method allows the detection of arenavirus antigens in various materials; organs and blood in affected animals and infected cell culture...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314603
更新日期：1981-01-01 00:00:00
abstract：:In order to investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains currently circulating in the Republic of Ireland (ROI), the ORF5 gene from 17 field strains originating from four vaccinating commercial herds was sequenced and phylogenetically analysed. High genetic v...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04710-7
更新日期：2020-09-01 00:00:00
abstract：:Phages, viruses targeting bacteria, have potential therapeutic applications in the control of infections with antibiotic-resistant bacteria. In this study, an Enterobacter cloacae phage, Ec_L1, was isolated from sewage sludge samples collected from a hospital. The genome of phage Ec_L1 consists of 51,894 bp with 48.24...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04672-w
更新日期：2020-08-01 00:00:00
abstract：:An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3681-x
更新日期：2018-04-01 00:00:00
abstract：:Since late 2018, foot-and-mouth disease (FMD) has reemerged and rapidly swept through pig farms in North and Central Vietnam, despite widespread use of commercial FMD vaccines. To investigate the FMD virus (FMDV) strains responsible for the current epidemics, 40 FMDV samples were collected from 17 provinces during Nov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04763-8
更新日期：2020-11-01 00:00:00
abstract：:The gene coding for the nucleocapsid protein NP of the influenza A virus recombinant strain 413 1,1 was characterized biochemically by molecular hybridization and fingerprint analysis. The data presented suggest that this NP gene has evolved by intracistronic recombination between NP genes of virus N and the fowl plag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322701
更新日期：1980-01-01 00:00:00
abstract：:A new bisegmented dsRNA virus has been detected in shoots of blue spruce with bud blight disease symptoms and infection by Cucurbitaria piceae (Bortw.) fungus. The virus genome consists of two segments that are 2071 and 2257 nt long, encoding the putative RNA polymerase and capsid protein, respectively. Rosellinia nec...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2692-8
更新日期：2016-05-01 00:00:00
abstract：:Two contigs with high similarity to partitivirus sequences were identified by de novo assembly of sequences obtained by RNA-Seq from a wild brassicaceous plant, Arabidopsis halleri subsp. gemmifera. Here, we report the complete genome sequence of a putative novel partitivirus. Excluding the poly-A tail, it consists of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04670-y
更新日期：2020-09-01 00:00:00
abstract：:Helper component protease (HC-Pro) is a potyvirus-encoded multifunctional protein and a major determinant of symptom expression in a susceptible plant. Here, we show the involvement of clover yellow vein virus (ClYVV) HC-Pro in necrotic symptom expression in broad bean (Vicia faba cv. Wase). In this host, lethal necro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1073-3
更新日期：2008-01-01 00:00:00
abstract：:Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuclease activity allo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315620
更新日期：1976-01-01 00:00:00
abstract：:The numbers of the carbohydrate side chains have been analyzed on a series of subtype 7 hemagglutinins of influenza A virus. The procedure employed involves growth of the virus in the presence of the trimming inhibitor N-methyl-1-desoxynojirimycin followed by controlled removal of the resulting mannose-rich oligosacch...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314433
更新日期：1987-01-01 00:00:00
abstract：:Fecal virus shedding, seroconversion and histopathology were evaluated in 3-7-year-old gnotobiotic calves orally inoculated with porcine deltacoronavirus (PDCoV) (9.0-9.6 log10 genomic equivalents [GE] of OH-FD22-P5; n=4) or porcine epidemic diarrhea virus (PEDV) (10.2-12.5 log10 GE of PC21A; n=3). In PDCoV-inoculated...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3351-z
更新日期：2017-08-01 00:00:00
abstract：:Two herpes simplex type 1 (HSV-1) recombinant viruses carrying the hepatitis C virus (HCV) NS5A open reading frame under the control of the cytomegalovirus immediate early (IE) or a herpes simplex chimeric promoter (alpha 4 gamma 1UL19) were constructed and characterized. Expression studies showed that both HSV-NS5A r...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0925-0
更新日期：2003-02-01 00:00:00
abstract：:A well characterized strain of guinea pig cytomegalovirus (GPCMV) was used to infect pregnant guinea pigs during various periods of pregnancy. Transplacental transmission of virus with invasion of the fetus was observed, even in some mothers with preinoculation evidence of GPCMV antibody. Fetal infection occurred duri...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317422
更新日期：1979-01-01 00:00:00
abstract：:Type I interferon (IFN) exerts strong antiviral activity, particularly against human immunodeficiency virus (HIV), and although several viral proteins have been shown to deregulate IFN induction, little is known about the induction of type I IFNs by HIV RNAs. In the present study, we used the stable isotope labeling w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2372-8
更新日期：2015-05-01 00:00:00
