Abstract：
:An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-monophosphate (UMP) as the endonuclease substrate and performed molecular dynamics simulations. The results demonstrated that the F35L mutation could result in a changed orientation of a helix containing active site residues and improve the ligand affinity in the mutant strain. This study suggests a molecular mechanism of enhanced polymerase activity.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Bhoye D,Cherian SSdoi
10.1007/s00705-017-3681-xsubject
Has Abstractpub_date
2018-04-01 00:00:00pages
1031-1036issue
4eissn
0304-8608issn
1432-8798pii
10.1007/s00705-017-3681-xjournal_volume
163pub_type
杂志文章abstract：:Styrian oil pumpkin seedlings (Cucurbita pepo L. subsp. pepo var. styriaca GREB: .) were treated for 48 h with 1 mM OTC (L-2-oxothiazolidine-4-carboxylic acid) in order to artificially increase cellular glutathione content. They were inoculated with zucchini yellow mosaic virus (ZYMV) 10 days later. The effects of OTC...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0880-2
更新日期：2007-01-01 00:00:00
abstract：:The susceptibility of mice of different ages (from four to 28 days) to infection with herpes simplex virus type 1 (HSV-1) mutants inoculated onto scarified corneas was studied. The TK+ isolate from wild type virus was pathogenic in mice of all age groups. An HSV-1 mutant (designated TK1/4) with a less active thymidine...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311326
更新日期：1983-01-01 00:00:00
abstract：:Triple gene block 1 (TGB1) and coat protein (CP) sequences of 30 hosta virus X (HVX) isolates from Tennessee (TN), USA, were determined and compared with available sequences in GenBank. The CPs of all known HVX isolates, including those from TN, shared 98.3-100% and 98.2-100% nucleotide and amino acid sequence identit...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0522-6
更新日期：2009-01-01 00:00:00
abstract：:A virus isolated from Olea europaea L. grown in Portugal, was identified as a member of the species Tobacco necrosis virus D (TNV-D, genus Necrovirus, family Tombusviridae), based on the molecular and serological properties of the purified virus particles. The genomic region encoding the coat protein (CP) of this isol...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0258-7
更新日期：2004-06-01 00:00:00
abstract：:We have recently demonstrated the geographic isolation of rice tungro bacilliform virus (RTBV) populations in the tungro-endemic provinces of Isabela and North Cotabato, Philippines. In this study, we examined the genetic structure of the virus populations at the tungro-outbreak sites of Lanao del Norte, a province ad...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070013
更新日期：2000-01-01 00:00:00
abstract：:Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314653
更新日期：1988-01-01 00:00:00
abstract：:In this study, a novel filamentous phage, φSHP1, of the environmental Stenotrophomonas maltophilia strain P2 was isolated and characterized. Electron microscopy showed that φSHP1 resembled members of the family Inoviridae and was about 2.1 μm long. The 6,867-nucleotide genome of φSHP1 was a circular single-stranded DN...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1305-z
更新日期：2012-09-01 00:00:00
abstract：:The complete genomes of tobamovirus isolates from Plantago major L. from New Zealand (NZ-439), Plantago sp. from Germany (Kons 1105), Actinidia chinensis (Actinidia-AC) and A. deliciosa (Actinidia-AD) from China were sequenced and compared to previously published tobamovirus genomes. Their genome organization and phyl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1292-0
更新日期：2012-07-01 00:00:00
abstract：:The plaque size and morphology of twenty-two influenza A virus recombinants representing seven distinct families were analyzed on MDCK cells. By examination of the genetic composition of the recombinants no relationship could be established between any gene, including those coding for the surface antigens, and the pla...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314716
更新日期：1982-01-01 00:00:00
abstract：:The ability of human fibroblast interferon to suppress colony formation and papovavirus T-antigen expression in six human tumor cell lines did not appear to correlate with their content of chromosomes 16 and 21. Additional factors or chromosomes may be involved in the manifestation of interferon action in aneuploid ce...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317505
更新日期：1979-01-01 00:00:00
abstract：:Multiple splicing sites exist in the RNA genome of the human immunodeficiency virus type 1 (HIV-1). In a screen for subgenomic forms of the HIV-1 genome that could be transferred to fresh cells by virus infection, we identified a novel spliced variant of HIV-1 RNA that uses a hitherto unknown splice acceptor site with...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718159
更新日期：1996-01-01 00:00:00
abstract：:Type I interferon (IFN) exerts strong antiviral activity, particularly against human immunodeficiency virus (HIV), and although several viral proteins have been shown to deregulate IFN induction, little is known about the induction of type I IFNs by HIV RNAs. In the present study, we used the stable isotope labeling w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2372-8
更新日期：2015-05-01 00:00:00
abstract：:Lolium latent virus (LoLV) was recently detected in the USA for the first time in ryegrass hybrids (Lolium perenne x Lolium multiflorum). The genome of one USA isolate, LoLV-US1, has now been fully sequenced. The positive strand genomic RNA is 7674 nucleotides (nt) long and is organized in five open reading frames (OR...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0108-8
更新日期：2008-01-01 00:00:00
abstract：:The complete genome of ϕ16, a temperate corynephage from Corynebacterium glutamicum ATCC 21792, was sequenced and annotated (GenBank: KY250482). The electron microscopy study of ϕ16 virion confirmed that it belongs to the family Siphoviridae. The ϕ16 genome consists of a linear double-stranded DNA molecule of 58,200 b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3383-4
更新日期：2017-08-01 00:00:00
abstract：:The gene coding for the nucleocapsid protein NP of the influenza A virus recombinant strain 413 1,1 was characterized biochemically by molecular hybridization and fingerprint analysis. The data presented suggest that this NP gene has evolved by intracistronic recombination between NP genes of virus N and the fowl plag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322701
更新日期：1980-01-01 00:00:00
abstract：:Utilizing co-transfection of DNA from glycoprotein gB- strain of HSV1 and cloned fragments of several simian alpha-herpesviruses containing the UL26, UL27 (gB glycoprotein), and UL28 gene homologs, replication-competent recombinant viruses were produced. Genetic analysis of one HSV1/SA8 recombinant (HSV1/SgB) demonstr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050114
更新日期：1997-01-01 00:00:00
abstract：:Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbre...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2635-4
更新日期：2016-01-01 00:00:00
abstract：:This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1673-z
更新日期：2013-08-01 00:00:00
abstract：:The complete nucleotide sequence (4838 nucleotides) of Iris yellow spot virus (IYSV) M RNA indicates, typical for tospoviruses, the presence of two genes in ambisense arrangement. The vRNA ORF codes for the potential cell-to-cell movement (NSm) protein (34.8 kDa) and the vcRNA ORF for the viral glycoprotein (G1/G2) pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0885-4
更新日期：2002-12-01 00:00:00
abstract：:As a prerequisite for development of improved vaccines and diagnostic tools for control of the fish pathogen koi herpesvirus, or cyprinid herpesvirus 3 (CyHV-3), we have started to identify putative viral envelope and capsid proteins. The complete or partial CyHV-3 open reading frames ORF25, ORF65, ORF92, ORF99, ORF13...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2190-4
更新日期：2014-12-01 00:00:00
abstract：:We determined the complete nucleotide sequence of EDRD-1, a Japanese strain of the North American type-Porcine reproductive and respiratory syndrome virus (PRRSV), and identified a novel 117-base deletion and 108-base insertion previously reported in the nsp2 gene of the SP strain, which contains the largest genome am...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0098-6
更新日期：2008-01-01 00:00:00
abstract：:A Bayesian phylogenetic analysis of eight separate gene segments indicated A/Swine/Shandong/2/2003 (H5N1), A/Swine/Shandong/na/2003 (H9N2), A/Swine/Shandong/nb/2003 (H9N2) and A/Swine/Shandong/nc/2005 (H9N2) probably represent two multiple reassortant lineages, that had not been described before, with genes coming fro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1083-1
更新日期：2008-01-01 00:00:00
abstract：:Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050675
更新日期：2000-01-01 00:00:00
abstract：:Chrysanthemums worldwide suffer from a high incidence of infection with chrysanthemum virus B (CVB), a member of the genus Carlavirus, family Betaflexiviridae. Three major lineages or strains of this virus have been found in India, but none have been characterized beyond the genetic variation they display in their coa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1190-x
更新日期：2012-03-01 00:00:00
abstract：:Cotton leaf curl disease (CLCuD) is a major limitation to cotton production on the Indian subcontinent. A survey for viruses causing CLCuD was conducted during the 2009 and 2010 cropping seasons in the northwestern Indian cotton-growing belt in the states of Punjab, Haryana and Rajasthan. Partial sequences of 258 and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1225-y
更新日期：2012-05-01 00:00:00
abstract：:A diagnostic assay to differentiate antibodies induced by foot-and-mouth disease virus (FMDV) infection from those induced by vaccination was developed. The test is an indirect-trapping ELISA which uses a monoclonal antibody to trap the non-structural 3ABC-FMDV polypeptide expressed in E. coli. Experimental and field ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050219
更新日期：1997-01-01 00:00:00
abstract：:Infection with West Nile virus and dengue virus, two mosquito-borne flaviviruses, is enhanced by two calcium-dependent lectins: dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), and its related molecule (DC-SIGNR). The present study examined the relationship between Japanese enc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2042-2
更新日期：2014-08-01 00:00:00
abstract：:We discovered a soybean mosaic virus (SMV) variant (4278-1) that caused systemic infections in Nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. The virus had a particle morphology and incubation period similar to those of other SMV isolates but differed from them in the leaf symptoms it ca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3123-1
更新日期：2017-02-01 00:00:00
abstract：:Tax oncoprotein of Human T-lymphotropic virus 1 (HTLV-1) has been proposed to dysregulate the expression of a number of cellular genes, many of which play a critical role for cell proliferation. Our initial data demonstrated that the immediate-early gene butyrate response factor 1 ( BRF1) was upregulated in HTLV-1-inf...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0114-9
更新日期：2003-09-01 00:00:00
abstract：:To provide information on the mechanism of attenuation of a Newcastle disease vaccine strain, TCND, we compared it with the parental virulent strain California 11,914 (CAL) biologically and genetically. It was found that TCND bore the fusion protein of virulent type, consisting of a pair of dibasic amino acid residues...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050361
更新日期：1998-01-01 00:00:00
