Abstract：
:The complete genome of ϕ16, a temperate corynephage from Corynebacterium glutamicum ATCC 21792, was sequenced and annotated (GenBank: KY250482). The electron microscopy study of ϕ16 virion confirmed that it belongs to the family Siphoviridae. The ϕ16 genome consists of a linear double-stranded DNA molecule of 58,200 bp (G+C = 52.2%) with protruding cohesive 3'-ends of 14 nt. Four major structural proteins were separated by SDS-PAGE and identified by peptide mass fingerprinting technique. Using bioinformatics analysis, 101 putative ORFs and 5 tRNA genes were predicted. Only 27 putative gene products could be assigned to known biological functions. The ϕ16 genome was divided into functional modules. Seven putative promoters and eight putative unidirectional intrinsic terminators were predicted. One site of putative «-1» programmed ribosomal frameshifting was proposed in the phage tail assembly genome region. C. glutamicum genetic tools could be broadened by exploiting the known integrase gene (gp33) and the newly identified excisionase gene (gp47), participating in site-specific recombination between ϕ16-attP/attB.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Lobanova JS,Gak ER,Andreeva IG,Rybak KV,Krylov AA,Mashko SVdoi
10.1007/s00705-017-3383-4subject
Has Abstractpub_date
2017-08-01 00:00:00pages
2489-2492issue
8eissn
0304-8608issn
1432-8798pii
10.1007/s00705-017-3383-4journal_volume
162pub_type
杂志文章abstract：:The combined use of phage and antibiotics can show synergistic antimicrobial effects, so-called phage-antibiotic synergy (PAS). Here, we screened and examined PAS against Pseudomonas aeruginosa in vitro. Testing four different phages infecting P. aeruginosa, phage KPP22 classified within the family Myoviridae genus Pb...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3811-0
更新日期：2018-07-01 00:00:00
abstract：:In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1214-6
更新日期：2012-04-01 00:00:00
abstract：:Replication of herpes simplex virus type 1 (HSV-1) in the adrenal gland of mice was observed 12 h after intravenous inoculation, peaked at 48 h (7 x 10(7) PFU/tissue), and was maintained until death. Virus spread to the bilateral intermediolateral column of the thoracic spinal cord. Infected cells appeared in the fasc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170048
更新日期：2001-10-01 00:00:00
abstract：:Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of duplicated TK DNA se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01378643
更新日期：1993-01-01 00:00:00
abstract：:Non-infectious hemagglutinating particles of rabies virus accumulated in the fluid phase of chick embryo cell cultures at 6 days post-infection, though they were undetectable at 4 days. They were characterized as looped filaments resembling viral envelope as revealed by electron microscopy. Another form of hemagglutin...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317937
更新日期：1976-01-01 00:00:00
abstract：:RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA speci...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1166-x
更新日期：2012-02-01 00:00:00
abstract：:During the epidemic outbreak in the region of Greifswald in the winter 1974/75, we found influenza virus variants which showed differences in the electrophoretic mobility of HA. Among the 25 isolates 13 were of slower and 12 of higher mobility. HA1 of 6 isolates was studied by determining the number of the carbohydrat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310484
更新日期：1991-01-01 00:00:00
abstract：:Heterogeneity of 9 feline herpesvirus type 1 (FHV-1) strains consisting of the prototype C27 strain, one French isolate, six Japanese isolates, and the attenuated vaccine F2 strain was examined by biological, immunological, and molecular biological methods. No significant difference was observed in virus growth and an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309701
更新日期：1992-01-01 00:00:00
abstract：:Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradeca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310506
更新日期：1990-01-01 00:00:00
abstract：:Analysis of the preS1 gene of hepatitis B virus was used to define two nosocomial outbreaks of HBV infection. In an outbreak in an oncology unit we had previously shown by single stranded conformational polymorphism (SSCP) analysis of a 189 bp fragment of the preS1 gene, that 52 children were infected with HBV strains...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050200
更新日期：1997-01-01 00:00:00
abstract：:We determined the nucleotide sequence of an 11059 bp fragment of the pseudorabies virus genome located in the right part of genomic BamHI fragment 3 and the adjacent part of BamHI fragment 6. Within this region eight open reading frames were identified whose deduced amino acid sequences exhibited homology to the UL6, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050056
更新日期：1997-01-01 00:00:00
abstract：:Integration of bovine leukaemia virus DNA was investigated at all stages of infection in cattle. We report here the detection of integrated proviral DNA in the majority of antibody positive animals without lymphocytosis. In all but one case virus was integrated at a number of different sites. Hybridization analysis fa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314319
更新日期：1991-01-01 00:00:00
abstract：:Rabies virus (RABV) isolates from two species of canids and three species of bats were analyzed by comparing the C-terminal region of the G gene and the G-L intergenic region of the virus genome. Intercluster identities for the genetic sequences of the isolates showed both regions to be poorly conserved. Phylogenetic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0671-7
更新日期：2010-06-01 00:00:00
abstract：:Strains of an influenza H10N4 virus have been isolated during an outbreak of a respiratory disease in mink on the south-east coast of Sweden. This was the first example of a disease in mammals caused by the H10 subtype. We compared the A/mink/Sweden/84 strain with two recent avian H10N4 isolates, one from fowl and ano...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318353
更新日期：1990-01-01 00:00:00
abstract：:A papovavirus was isolated from the urine of a 24 year old female who underwent bone marrow transplantation after a relapse of acute myeloid leukemia. The virus (JL) resembles BK virus in its antigenic and growth properties, but has a different restriction endonuclease pattern after digestion with the restriction endo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315635
更新日期：1978-01-01 00:00:00
abstract：:Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HER...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0764-5
更新日期：2006-10-01 00:00:00
abstract：:Recently we have described that the Herpes simplex virus (HSV)-induced thymidine kinase (TK) induces AMP- and ADP-dThd-5'-phosphotransferase activities. We now demonstrate the heterogeneity of the described activities in isoelectric focusing experiments and polyacrylamide gel electrophoresis. A TK--mutant of HSV type ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314875
更新日期：1982-01-01 00:00:00
abstract：:Colons from ducks and chicken 1, 7, 14 and 28 days old maintained near-normal morphology up to 48 and 96 hours respectively in a system using NCTC135 medium (a part) + Dulbecco's modified Eagle's medium (9 parts ), at 37 degrees C and 95 per cent O2/5 per cent CO2. In the colon of 1 and 28 day-old ducks, duck influenz...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315007
更新日期：1981-01-01 00:00:00
abstract：:We evaluated the importance of dendritic cells (DCs) in the induction of the immune response after immunization of mice with DNA plasmid Auxo-GTU(®)-MultiHIV. First, GTU(®)-encoded protein was shown to be expressed by DCs of the draining lymph nodes (LNs) following intradermal (i.d.) immunization. Next, donor mice wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1003-2
更新日期：2011-09-01 00:00:00
abstract：:Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309576
更新日期：1992-01-01 00:00:00
abstract：:Begomoviruses have been detected infecting the weed Cnidoscolus urens (family Euphorbiaceae) since 2004, but the viral species to which these viruses belonged was not known. Here, we report for the first time the complete genome sequence of a bipartite begomovirus obtained from C. urens collected in the state of Alago...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2919-3
更新日期：2016-09-01 00:00:00
abstract：:A Luminex xTAG-based assay for plant-infecting tospoviruses was developed. The test enables the detection of tospoviruses in general and the differentiation of the four important member species of this genus: Tomato spotted wilt virus, Impatiens necrotic spot virus, the proposed 'Capsicum chlorosis virus' and Watermel...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3256-x
更新日期：2017-06-01 00:00:00
abstract：:The genus Bromovirus currently contains six species whose members have relatively narrow host ranges. In the present work, a new bromovirus infecting elderberry (Sambucus nigra L.) is reported. dsRNA was purified and sequenced by next-generation sequencing, and with minimal additional completion by Sanger sequencing, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3629-1
更新日期：2018-02-01 00:00:00
abstract：:The complete nucleotide sequence of genome segment S9, which was reported as the ninth largest among the 12 genome segments of rice dwarf virus, was determined. The segment was 1,305 bp long and coded for a protein composed of 351 amino acids (Mr 38,930). The terminal sequences of the segment were found to consist of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313885
更新日期：1989-01-01 00:00:00
abstract：:Mucosal disease (MD) can be induced in cattle persistently infected with noncytopathogenic bovine viral diarrhea virus (ncp BVD virus) by superinfecting them with antigenically related cytopathogenic (cp) BVD virus strains. While some of these animals succumb to early onset MD after 2 to 3 weeks post infectionem (p.i....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050322
更新日期：1998-01-01 00:00:00
abstract：:The genome of adenovirus (AV) types 1, 2, and 5 is known to be very variable as evidenced by the great number of genome types described (36, 61, and 35 for AV1, 2, and 5 respectively). Physical maps were constructed for nearly all of the restriction or R-variants by biochemical methods and by adapting restriction frag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718606
更新日期：1996-01-01 00:00:00
abstract：:In 1991 and 1992, H1N2 influenza A viruses were isolated from the lungs of pigs with overt signs of respiratory disease at farms in the Chiba and Kanagawa prefectures of Japan. To determine the genetic origin of these isolates, we phylogenetically analyzed partial nucleotide sequences of their genes. The results indic...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050415
更新日期：1998-01-01 00:00:00
abstract：:Conditions for ELISA by using IgG linked with horseradish peroxidase have been developed for the detection of 6 arenaviruses (Machupo, Junin, Tacaribe, Amapari, LCM and Tamiami). This method allows the detection of arenavirus antigens in various materials; organs and blood in affected animals and infected cell culture...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314603
更新日期：1981-01-01 00:00:00
abstract：:3Y1 rat fibroblasts transformed by adenovirus type 12 or by its E1A gene formed syncytia when cocultivated with the Friend as well as other ecotropic murine leukemia-virus-producing cells. In contrast, the parental 3Y1 cells and those transformed by other tumor viruses or by a chemical carcinogen exhibited no syncytiu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310628
更新日期：1990-01-01 00:00:00
abstract：:We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, in breeding lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) collected from the two centers of common bean domestication: Mesoamerica and the Andes. Th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2335-0
更新日期：2015-04-01 00:00:00
