Abstract:
:A reversed passive hemagglutination (RPHA) method was developed for the detection of bovine coronavirus in fecal specimens. Sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine coronavirus linked to Sepharose 4B. The RPHA test was carried out by a microtiter method. Erythrocytes coated with purified specific antibodies were agglutinated by bovine coronavirus, but not by bovine rotavirus or enterovirus. The reaction was inhibited by antiserum to bovine coronavirus, confirming the specificity of the reaction. The RPHA test detected bovine coronavirus in 13 of 22 fecal specimens (59 per cent), from natural cases of diarrhea, while the positive rates were only 14 per cent (3/22) and 22 per cent (5/22) for immunofluorescent staining of primary cultures of calf kidney cells infected with the specimens, and immune electron microscopy respectively. The advantages of the RPHA method are its simplicity, high sensitivity and rapidity.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Sato K,Inaba Y,Tokuhisa S,Miura Y,Kaneko N,Asagi M,Matumoto Mdoi
10.1007/BF01315291subject
Has Abstractpub_date
1984-01-01 00:00:00pages
23-31issue
1eissn
0304-8608issn
1432-8798journal_volume
80pub_type
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
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doi:10.1007/s00705-012-1597-z
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
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doi:10.1007/BF01348017
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journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313877
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journal_title:Archives of virology
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journal_title:Archives of virology
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doi:10.1007/s007050050535
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pub_type: 杂志文章
doi:10.1007/s00705-013-1912-3
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journal_title:Archives of virology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1007/BF01320246
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322691
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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