Abstract:
:Adenovirus type 7 vaccine strain was engineered to express foreign antigens from both the E3 early promoter in the E3 region and the major late promoter inserted between the E4 region and the right inverted terminal repeat. This multiple expression vector was used to express hepatitis B core antigen (HBcAg), hepatitis B e antigen (HBeAg), and hepatitis B surface antigen (HBsAg). The gene inserted in the E3 region was derived from the core gene of the hepatitis B virus genome. When the precore region was present, an immunoreactive group of proteins with molecular weights ranging from 15,000 to 19,000 was secreted into the media. Velocity sedimentation centrifugation of media and lysates from cells infected with recombinants containing the core gene with the precore region resulted in peaks of HBeAg at the top of the gradient where authentic HBeAg should be found. In addition to the core gene in the E3 region, the surface antigen gene of hepatitis B virus was inserted behind the major late promoter in the E4 region resulting in an adeno-hepatitis recombinant virus capable of expressing both the core gene and the HBsAg cells. Cells infected with the adeno-hepatitis recombinants could also be stained with peroxidase-conjugates after reacting to antibody against HBcAg. Inoculation of dogs with the recombinant viruses which contained the core gene, with and without the precore sequence, resulted in a significant antibody response to HBcAg/HBeAg. The dogs also produced a significant antibody response to HBsAg as well as neutralizing antibody to adenovirus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Ye WW,Mason BB,Chengalvala M,Cheng SM,Zandle G,Lubeck MD,Lee SG,Mizutani S,Davis AR,Hung PPdoi
10.1007/BF01311300subject
Has Abstractpub_date
1991-01-01 00:00:00pages
11-27issue
1-2eissn
0304-8608issn
1432-8798journal_volume
118pub_type
杂志文章abstract::A rotavirus-like virus has been isolated from cases of neonatal diarrhoea in piglets. No antigenic relationship with known rotaviruses or with an American strain of a rotavirus-like virus has been demonstrated. Morphologically the virus is similar to known rotaviruses, but it differs in the ability to form syncytia of...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311220
更新日期:1984-01-01 00:00:00
abstract::An electron microsopic study of cell cultures and bovine foetal tracheal organ cultures infected with infectious bovine rhinotracheitis (IBR) virus showed the following. a) The difference in the site at which the outer envelope is acquired in the infected cell is responsible for some morphological differences between ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317842
更新日期:1976-01-01 00:00:00
abstract::Seven isolates of West Nile virus and an additional isolate tentatively identified at the outset as West Nile virus, were compared by Casals' modified haemagglutination inhibition technique. Two of the isolates were nearly identical; a second set of five isolates cross-reacted among themselves within a wide range and ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315279
更新日期:1978-01-01 00:00:00
abstract::Recently, five new viruses from the genus Vitivirus were identified and named grapevine virus G, H, I, J and L. These viruses were targeted in a survey to evaluate their prevalence in different grapevine populations in California. Excluding a single detection of GVJ, other vitiviruses were detected infecting several g...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04355-1
更新日期:2019-10-01 00:00:00
abstract::The interaction between the nucleocapsid (N) protein of mouse hepatitis virus (MHV) and RNA was studied in an effort to define portions of the N molecule that participate in binding to RNA. N mRNAs transcribed from SP6 and T7 vectors were translated in a rabbit reticulocyte lysate. Analysis of synthesized N protein in...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309634
更新日期:1992-01-01 00:00:00
abstract::By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to geno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0679-z
更新日期:2010-07-01 00:00:00
abstract::A nucleotide fragment encoding amino acids 29 to 94 in the E-protein of 28 dengue-2 isolates of diverse geographic and host origins was examined by direct sequencing of a polymerase chain reaction (PCR)-amplified product, and compared to six previously published sequences. Nucleotide divergence ranged from 0 to 19.8% ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316895
更新日期:1993-01-01 00:00:00
abstract::Two contigs with high similarity to partitivirus sequences were identified by de novo assembly of sequences obtained by RNA-Seq from a wild brassicaceous plant, Arabidopsis halleri subsp. gemmifera. Here, we report the complete genome sequence of a putative novel partitivirus. Excluding the poly-A tail, it consists of...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04670-y
更新日期:2020-09-01 00:00:00
abstract::In order to improve the knowledge of the humoral immune response to CMV infection, we developed an immunoblotting technique which allowed a better analysis of the changes in the pattern of anti CMV-polypeptides IgM. We examined 234 sera belonging to 27 renal allograft recipients developing a primary or recurrent CMV i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310938
更新日期:1989-01-01 00:00:00
abstract::The recent H5N1 avian influenza outbreaks in Asia spread over more than 8 countries. It has caused enormous economic loss and grand challenges for the public health. During these breakouts we isolated three strains of H5N1 Avian Influenza Virus (AIV) from chickens and one from duck in different farms of Southern China...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0474-9
更新日期:2005-06-01 00:00:00
abstract::Infectious laryngotracheitis (ILT), caused by infectious laryngotracheitis virus (ILTV), is an Office International des Epizooties (OIE) notifiable disease. However, we have not clearly understood the dynamic distribution, tissue tropism, pathogenesis, and replication of ILTV in chickens. In this report, we investigat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1414-8
更新日期:2013-03-01 00:00:00
abstract::Dengue virus is the most important arbovirus that affects humans, and it can establish persistent infections, especially in insect-derived cell cultures. Defective viral genomes have been implicated in the establishment and maintenance of persistent infections with several flaviviruses; however, there exists almost no...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1525-2
更新日期:2013-03-01 00:00:00
abstract::A novel plant virus with a positive single-stranded (+ss) RNA genome was detected in Taibei pomelo (Citrus grandis (L.) Osbeck cv. Taibeiyou) in China by high-throughput sequencing (HTS). Tentatively named "citrus yellow spot virus" (CiYSV), it has 8,061 nucleotides (nt) excluding the poly(A) tail and contains three o...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04794-1
更新日期:2020-11-01 00:00:00
abstract::Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent memb...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050332
更新日期:1998-01-01 00:00:00
abstract::Human papillomavirus (HPV) type 13 DNA was detected in focal epithelial hyperplasia lesions of the oral mucosa in seven half-caste mexicans. The lesions contained intracellular papillomavirus-like particles with a diameter of about 50 nm. DNA extracted from biopsies contained unintegrated HPV type 13 DNA genomes as re...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313899
更新日期:1987-01-01 00:00:00
abstract::Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy cal...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04792-3
更新日期:2020-11-01 00:00:00
abstract::A novel strain of Japanese yam mosaic virus (JYMV-CN) was identified in a yam plant with foliar mottle symptoms in China. The complete genomic sequence of JYMV-CN was determined. Its genomic sequence of 9701 nucleotides encodes a polyprotein of 3247 amino acids. Its organization is virtually identical to that of two J...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2231-z
更新日期:2015-02-01 00:00:00
abstract::Mice are protected against lethal intraperitoneal and intravenous infection by encephalomyocarditis virus and Semliki Forest virus by sequential treatment with poly I followed by either polyC or poly5-hydroxyC without production of interferon when the treatments are 4 or more hours apart and by the intraperitoneal or ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318024
更新日期:1976-01-01 00:00:00
abstract::Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined. The genome is 9,544 nucleotides long excluding the 3' terminal poly A tail and encodes a typical potyviral 350-kDa polyprotein of 3,087 amino acids. Phylogenetic an...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0735-x
更新日期:2006-08-01 00:00:00
abstract::Mouse cell lines (L, NIH3T3, and RMA cells) infected with the Edmonston strain of measles virus (MV) did not exhibit cytopathic effects (CPE), consistent with the finding that mice are not susceptible to MV. Northern blot analysis, however, revealed that MV genes were transcribed in infected L and NIH3T3 cells, althou...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310037
更新日期:1994-01-01 00:00:00
abstract::Two strains of mouse cytomegalovirus (MCMV) propagated in mouse salivary glands could be distinguished in neutralization tests. Cell (mouse embryo fibroblast)-passaged virus of either strain was generally 10-20 times easier to neutralize than virus produced in mouse salivary glands. Evidence was obtained that mouse im...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315153
更新日期:1981-01-01 00:00:00
abstract::Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04842-w
更新日期:2020-12-01 00:00:00
abstract::A potyvirus isolated from Pinellia ternata in China was characterised and shown to be related to Soybean mosaic virus (SMV). The virus was pathogenic on P. ternata and some soybean cultivars, whereas the local soybean SMV isolate HH5 did not infect P. ternata. Western blot experiments demonstrated a serological relati...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0184-8
更新日期:2004-02-01 00:00:00
abstract::Yellow mosaic disease was observed on Jatropha gossypifolia plants growing in Kathaupahadi, Madhya Pradesh, India, and whiteflies (Bemisia tabaci) were found in the vicinity. Association of a new begomovirus with yellow mosaic disease of J. gossypifolia has been detected by PCR using begomovirus DNA-A-specific primers...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1118-5
更新日期:2011-12-01 00:00:00
abstract::Euthymic (SD or outbred rnu/+) and athymic (rnu/rnu) rats were inoculated oronasally or intraperitoneally with the RV-Y strain of rat virus when they were 2 days or 4 weeks old. Clinical signs of infection in athymic infants were similar to those in euthymic infants, but significantly more athymic infants died. Some i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311362
更新日期:1989-01-01 00:00:00
abstract::BALB/c mice developed contralateral necrotizing retinitis following intracameral inoculation with herpes simplex virus type 1 (HSV-1). The animals showed a positive delayed-type hypersensitivity (DTH) response at 10 days postinoculation, indicating that the anterior chamber-associated immune deviation was transient af...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309535
更新日期:1993-01-01 00:00:00
abstract::This study reports the molecular epidemiology and genetic characterization of human respiratory syncytial virus (RSV) samples collected in Thailand from January 2010 to December 2011. In total, 1,315 clinical samples were collected from Bangkok and Khon Kaen provinces and were screened by semi-nested PCR for RSV infec...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1773-9
更新日期:2014-03-01 00:00:00
abstract::Bacteriophage JBP901, isolated from fermented food, is specific for Bacillus cereus group species and exhibits a broad host spectrum among a large number of B. cereus isolates. Genome sequence analysis revealed a linear 159,492-bp genome with overall G+C content of 39.7 mol%, and 201 ORFs. The presence of a putative m...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2485-0
更新日期:2015-09-01 00:00:00
abstract::Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314741
更新日期:1980-01-01 00:00:00
abstract::The DNAs from nine Japanese field isolates of equine herpesvirus-1 (EHV-1) were analyzed by digestion with the restriction endonuclease Bam HI and Southern hybridization. Comparing restriction profiles among the EHV-1 strains, there was no considerable difference between isolates before and after vaccine application, ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316881
更新日期:1993-01-01 00:00:00