Abstract：
:One host polypeptide (40,000 daltons) synthesized prior to infection is associated with the 250S RNA polymerase structure partially purified by a combination of velocity sedimentation and isopycnic separation. A series of pulse-chase experiments have shown that a 56,000 dalton polypeptide made during the eclipse phase of infection is inserted into the 250S viral RNA polymerase structure. This 56,000 dalton polypeptide is bound in a stable manner since labeled 56,000 dalton polypeptide is not removed from the 250S polymerase structure by a 2-hour chase (3 to 5 hours after infection) and it is the major labeled polypeptide species remaining. However, the 56,000 dalton polypeptide (viral-specific polypeptide E) made at 4 hours after infection is not present in the 250S polymerase structure following a 50 minue chase. Levels of cycloheximide which inhibit protein synthesis 95 per cent in the infected cell have no effect on the amount of viral-specific RNA polymerase activity (in vitro) when the inhibitor is added for 30 minutes at the time of maximum rate of viral RNA synthesis in whole cells. These inhibitor studies support the hypothesis that the viral-specific RNA polymerase polypeptide may be a stable polypeptide that is not rapidly turning over in the infected cell. In view of these results the stable 56,000 dalton polypeptide (polypeptide E) made early in infection may be a candidate for the viral-specific polymerase polypeptide.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Loesch WT Jr,Arlinghaus Adoi
10.1007/BF01317808keywords:
subject
Has Abstractpub_date
1975-01-01 00:00:00pages
201-15issue
3eissn
0304-8608issn
1432-8798journal_volume
47pub_type
杂志文章abstract：:The E1 nucleic acid sequence of rubella virus strain Judith (RJ) has been cloned into an E. coli expression vector LB03. The reactivity of the expressed unglycosylated antigen (E1J) was compared with its glycosylated counterpart in native virus (RJ) using rabbit and human sera. Rabbit antisera raised against RJ and E1...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317201
更新日期：1992-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3509-8
更新日期：2017-11-01 00:00:00
abstract：:Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simulta...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-016-3065-7
更新日期：2017-01-01 00:00:00
abstract：:To provide information on the mechanism of perpetuation of influenza viruses among waterfowl reservoirs in nature, virological surveillance was carried out in Alaska during their breeding season in summer from 1991 to 1994. Influenza viruses were isolated mainly from fecal samples of dabbling ducks in their nesting pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322743
更新日期：1995-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718424
更新日期：1995-01-01 00:00:00
abstract：:A total of 72 Borna Disease virus infected rabbits were treated with different concentrations of cyclophosphamide, glucocorticoids or both in combination. Comparison with untreated, infected rabbits showed a drastic alteration in the clinical picture, a considerable prolongation of the survival time, and differences i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318132
更新日期：1981-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314716
更新日期：1982-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4011-7
更新日期：2018-12-01 00:00:00
abstract：:Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as probes. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309448
更新日期：1993-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0010-9
更新日期：2008-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0330-y
更新日期：2004-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310704
更新日期：1990-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315171
更新日期：1982-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04205-0
更新日期：2019-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0368-x
更新日期：2004-12-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0685-8
更新日期：2006-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314609
更新日期：1981-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314603
更新日期：1981-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0898-z
更新日期：2003-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0222-7
更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-019-04346-2
更新日期：2019-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-019-04493-6
更新日期：2020-02-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2930-8
更新日期：2016-09-01 00:00:00
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journal_title：Archives of virology
pub_type： 新闻
doi：10.1007/s00705-020-04731-2
更新日期：2020-12-01 00:00:00
abstract：:A comparative study of the ability of three low-pathogenic avian influenza virus (LPAIV) isolates to be transmitted from duck to duck was performed. Pekin ducks were inoculated with two LPAIV isolates from chickens (A/Ck/PA/13609/93 [H5N2], H5N2-Ck; A/Ck/TX/167280-4/02 [H5N3], H5N3-Ck) and one isolate from a wild bird...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2504-1
更新日期：2015-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1227-9
更新日期：2012-04-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314741
更新日期：1980-01-01 00:00:00
abstract：:Eco RI fragment G of equine herpesvirus 4 strain 405/76 (EHV 4.405/76) is located at the left end of the unique short region close to or extending into the internal repeat region of the prototypic arrangement of the genome. The nucleotide sequence of two subclones designated HS and G 19, contiguous within Eco RI fragm...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309795
更新日期：1993-01-01 00:00:00
abstract：:Here, we report the molecular characterization of a novel partitivirus from Phomopsis vexans strain PvHZ002, a plant-pathogenic fungus infecting eggplant. The virus was designated "Phomopsis vexans partitivirus 1" (PvPV1). PvPV1 contains two dsRNA segments, dsRNA1 and dsRNA2, which are 1,662 bp and 1,628 bp long, resp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04835-9
更新日期：2021-01-01 00:00:00
abstract：:Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major anti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070087
更新日期：2000-01-01 00:00:00
