Abstract:
:The receptor for adeno-associated virus serotype 2 (AAV2) in Purkinje cells has not been identified, but based on work carried out in non-neuronal cell lines, heparan sulfate proteoglycan is thought to act as a primary receptor, with basic fibroblast growth factor receptor-1 being reported as a co-receptor. In this study, using antibody interference and protein competition strategies, we show specific reduction in Purkinje cell transduction by AAV2 vector in the presence of these inhibitors. We also demonstrate AAV2-mediated transgene expression in Purkinje cells in vivo that extends out to one year post-injection. These results provide new information on fibroblast growth factor receptor-1 involvement in AAV2-mediated transduction of Purkinje cells and have important implications for AAV-mediated treatment of various cerebellar disorders.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Belur LR,Kaemmerer WF,McIvor RS,Low WCdoi
10.1007/s00705-008-0222-7subject
Has Abstractpub_date
2008-01-01 00:00:00pages
2107-10issue
11eissn
0304-8608issn
1432-8798journal_volume
153pub_type
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journal_title:Archives of virology
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