Abstract:
:This work describes the first molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars. During a routine screening of 44 accessions in the National Collection of Grapevine Varieties (CAN PRT051), 17 were found infected with GVB in DAS-ELISA assays with commercial antibodies. However, only six of the corresponding isolates were successfully amplified using primer pairs described in the literature. The sequence variants (ORF4-3'UTR, 1147 nt) retrieved from these isolates segregated into two phylogenetic groups, which included sequences from complete genomes available in GenBank. The highly discrepant results obtained using serological and RT-PCR-based diagnostic tools led to the design of a primer pair for detection of GVB, which allowed the amplification of a 606-bp GVB-specific fragment from all DAS-ELISA-positive isolates and also revealed the existence of false negatives in the serological testing.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Fonseca F,Duarte V,Teixeira Santos M,Brazão J,Eiras-Dias Edoi
10.1007/s00705-016-3024-3subject
Has Abstractpub_date
2016-12-01 00:00:00pages
3535-3540issue
12eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-3024-3journal_volume
161pub_type
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