Abstract:
:Both H9N2 subtype avian influenza and 2009 pandemic H1N1 viruses (pH1N1) can infect humans and pigs, which provides the opportunity for virus reassortment, leading to the genesis of new strains with potential pandemic risk. In this study, we generated six reassortant H9 viruses in the background of three pH1N1 strains from different hosts (A/California/04/2009 [CA04], A/Swine/Jiangsu/48/2010 [JS48] and A/Swine/Jiangsu/285/2010 [JS285]) by replacing either the HA (H9N1-pH1N1) or both the HA and NA genes (H9N2-pH1N1) from an h9.4.2.5-lineage H9N2 subtype influenza virus, A/Swine/Taizhou/5/08 (TZ5). The reassortant H9 viruses replicated to higher titers in vitro and in vivo and gained both efficient transmissibility in guinea pigs and increased pathogenicity in mice compared with the parental H9N2 virus. In addition, differences in transmissibility and pathogenicity were observed among these reassortant H9 viruses. The H9N2-pH1N1viruses were transmitted more efficiently than the corresponding H9N1-pH1N1 viruses but showed significantly decreased pathogenicity. One of the reassortant H9 viruses that were generated, H9N-JS48, showed the highest virulence in mice and acquired respiratory droplet transmissibility between guinea pigs. These results indicate that coinfection of swine with H9N2 and pH1N1viruses may pose a threat for humans if reassortment occurs, emphasizing the importance of surveillance of these viruses in their natural hosts.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
He L,Wu Q,Jiang K,Duan Z,Liu J,Xu H,Cui Z,Gu M,Wang X,Liu X,Liu Xdoi
10.1007/s00705-014-2009-3subject
Has Abstractpub_date
2014-07-01 00:00:00pages
1743-54issue
7eissn
0304-8608issn
1432-8798journal_volume
159pub_type
杂志文章abstract::The complete nucleotide sequence of the RNA genome of turnip mosaic virus Japanese strain (TuMV-J) has been determined from five overlapping cDNA clones and by direct sequencing of viral RNA. The RNA sequence was 9833 nucleotides in length, excluding a 3' terminal poly(A) tail. An AUG triplet at position 130-132 was a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718209
更新日期:1996-01-01 00:00:00
abstract::The complete genome sequences of isolates of two new bipartite begomoviruses (genus Begomovirus, family Geminiviridae) found infecting common bean in Venezuela are provided. The names proposed for each of these viruses are "bean yellow chlorosis virus" (BYCV) and "bean white chlorosis mosaic virus" (BWCMV). Phylogenet...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1545-y
更新日期:2013-03-01 00:00:00
abstract::Four epidemic seasons of rotaviruses were studied in Helsinki during 1986-1990. This is the first Scandinavian study, where both electropherotypes and serotypes are determined. Out of 5316 fecal specimens 769 (14.5%) rotavirus positive samples were detected by electron microscopy. Of these, 645 isolates (83.9%) gave a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314964
更新日期:1995-01-01 00:00:00
abstract::Twenty TGEV isolates were compared by sequencing a 393-414 nucleotide stretch near the 5' end of the S gene, after amplification by RT-PCR. This part of the S gene is known to show considerable variation between porcine, canine and feline coronaviruses and is completely deleted from porcine respiratory coronaviruses. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050191
更新日期:1997-01-01 00:00:00
abstract::Although surfactant-secreting type II alveolar cells have been shown to be damaged during influenzavirus pneumonia, little is known about the effects of surfactant replacement therapy. We have developed a mouse influenza model, in which viral infection can be localized to the upper respiratory tract or to both the upp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050535
更新日期:1999-01-01 00:00:00
abstract::Knowledge of coronaviruses in wild carnivores is limited. This report describes coronavirus genetic diversity, species specificity and infection prevalence in three wild African carnivores. Coronavirus RNA was recovered from fresh feces from spotted hyena and silver-backed jackal, but not bat-eared fox. Analysis of se...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1562-x
更新日期:2013-04-01 00:00:00
abstract::During the surveillance for avian influenza viruses (AIVs) in live poultry markets in Eastern China, in 2016, a novel reassortant H7N6 AIV was isolated from a chicken. Phylogenetic analysis showed that this strain received its genes from H9N2, H7N9 and H5N6 AIVs infecting poultry in China. This strain showed moderate ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3219-2
更新日期:2017-05-01 00:00:00
abstract::A Bayesian phylogenetic analysis of eight separate gene segments indicated A/Swine/Shandong/2/2003 (H5N1), A/Swine/Shandong/na/2003 (H9N2), A/Swine/Shandong/nb/2003 (H9N2) and A/Swine/Shandong/nc/2005 (H9N2) probably represent two multiple reassortant lineages, that had not been described before, with genes coming fro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1083-1
更新日期:2008-01-01 00:00:00
abstract::Chimeric plant viruses are emerging as promising vectors for use in innovative vaccination strategies. In this context, cucumber mosaic virus (CMV) has proven to be a suitable carrier of the hepatitis C virus (HCV)-derived R9 mimotope. In the present work, a new chimeric CMV, expressing on its surface the HCV-derived ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0916-7
更新日期:2007-01-01 00:00:00
abstract::The nucleotide sequences of the SH gene of 45 new mumps virus isolates derived from different parts of Europe, Canada and USA were determined. A phylogenetic tree was constructed which confirmed the existence of three major clusters. While clustering according to geographical origin was clear in some cases each of the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050073
更新日期:1997-01-01 00:00:00
abstract::Bacterial endotoxins, DNA, live viruses, and viral proteins derived from bacterial and baculovirus (BV) expression vectors employed in recombinant protein production contaminate the final product. Density gradient centrifugation is commonly used to partially purify oligomeric proteins, but impurities from the expressi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2991-8
更新日期:2016-11-01 00:00:00
abstract::The influenza season 2014/15 was dominated by drift variants of influenza A(H3N2), which resulted in a reduced vaccine effectiveness. It was not clear if the performance of commercial nucleic-acid-based amplification (NAT) assays for the detection of influenza was affected. The purpose of this study was to perform a r...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2930-8
更新日期:2016-09-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection often leads to liver cancer. The HCV NS2 protein is a hydrophobic transmembrane protein that associates with several cellular proteins in mammalian cells. In this report, we investigated the function of NS2 protein on HCV replication and translation by using a transient cell-b...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0198-3
更新日期:2008-01-01 00:00:00
abstract::Sixteen patients with symptoms typical for Ockelbo disease (rash, arthralgia, fever) were enrolled in a 2 1/2 year study, during which clinical symptoms were recorded and ELISA was employed to study specific IgM, IgG and IgG subclass development. Initially, all patients presented with rash and arthralgia, and five pat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379107
更新日期:1994-01-01 00:00:00
abstract::Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3260-1
更新日期:2017-07-01 00:00:00
abstract::A group of previously isolated heterogeneous mEp lambdoid phages (43) from 19 different immunity groups for phage infection was further characterized to gain insight into some phenotypic traits and to assess their relationship with phage lambda. Interestingly, the FhuA host receptor was required by the majority of mEp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0111-0
更新日期:2008-01-01 00:00:00
abstract::Anti-human T-lymphotropic virus type I/II (HTLV-I/II) antibodies were screened by particle agglutination test in a total of 66 patients with thalassemia major who received multiple transfusion from paid donors at the Blood Transfusion Hematology Center of Ho Chi Minh City in South Vietnam. HTLV-II infection was confir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050170
更新日期:1997-07-01 00:00:00
abstract::Treatment of a mouse macrophage cell line Mk1 with pokeweed mitogen (PWM) either before or during but not after virus inoculation resulted in an enhancement of dengue virus (DV) infection. The infection enhancement was primarily due to an increase in the number of DV-infected cells but not to increased virus productio...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379106
更新日期:1994-01-01 00:00:00
abstract::Recombinants with known gene constellations between fowl plague virus (FPV) and various prototype influenza virus strains have been examined for neurovirulence in suckling mice. Strongly neurotropic recombinants were obtained from crosses between FPV and the strains virus N, Hong Kong, and PR8, but not between FPV and...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314891
更新日期:1983-01-01 00:00:00
abstract::The genome of Japanese iris necrotic ring virus (JINRV) consists of a positive-sense ssRNA of 4014 nucleotides with six major open reading frames (ORFs). A 5'-non-coding region of 31 nucleotides precedes the first initiation codon. Like Carnation mottle virus (CarMV), the 5'-proximal three ORFs encode a 26 kDa protein...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050054
更新日期:2000-01-01 00:00:00
abstract::Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2605-x
更新日期:2015-12-01 00:00:00
abstract::The complete genome of a novel mycovirus, Puccinia striiformis mitovirus 1 (PsMV1), derived from the wheat stripe rust fungus Puccinia striiformis strain SCSN-10, was sequenced and analyzed. The full-length cDNA sequence is 2496 bp in length with a predicted AU content of 57.65% in the genomic RNA. Sequence analysis i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-04134-4
更新日期:2019-03-01 00:00:00
abstract::Coxsackievirus B (CVB) can cause aseptic meningitis, myocarditis and respiratory disease, especially in newborn infants. To compare the susceptibility to CVB infection of fetal and adult mice, we prepared primary alveolar epithelial cells (AECs) from lungs of BALB/c mice. In contrast to fetal mouse AECs, those of adul...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1254-6
更新日期:2012-06-01 00:00:00
abstract::Rabies virus (RABV) isolates from two species of canids and three species of bats were analyzed by comparing the C-terminal region of the G gene and the G-L intergenic region of the virus genome. Intercluster identities for the genetic sequences of the isolates showed both regions to be poorly conserved. Phylogenetic ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0671-7
更新日期:2010-06-01 00:00:00
abstract::The objectives of this study were to determine the antigenic relationship among ruminant adenoviruses and determine their phylogenetic relationship based on the deduced hexon gene amino acid sequence. Results of reciprocal cross-neutralization tests demonstrated antigenic relationships in either one or both directions...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0063-4
更新日期:2008-01-01 00:00:00
abstract::A reversed passive hemagglutination (RPHA) method was developed for the detection of bovine coronavirus in fecal specimens. Sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine coronav...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315291
更新日期:1984-01-01 00:00:00
abstract::Bovine leukemia virus (BLV) causes enzootic bovine leucosis (EBL) and is responsible for substantial economic losses in cattle globally. However, information in Africa on the disease is limited. Here, based on clinical, hematological, pathological and molecular analyses, two clinical cases of EBL were confirmed in a d...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3205-0
更新日期:2017-04-01 00:00:00
abstract::Drug resistance mutations in the Pol gene of human immunodeficiency virus 1 (HIV-1) are one of the critical factors associated with antiretroviral therapy (ART) failure in HIV-1 patients. The issue of resistance to reverse transcriptase inhibitors (RTIs) in HIV infection has not been adequately addressed in the Indian...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2760-8
更新日期:2016-05-01 00:00:00
abstract::The CPK cells derived from swine kidney were infected with the attenuated TO-163 strain of transmissible gastroenteritis (TGE) virus, and fused with uninfected Vero cells in the presence of polyethylene glycol. Repeated cocultivation of the fused cells with uninfected Vero cells rendered the virus to grow in Vero cell...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01321128
更新日期:1992-01-01 00:00:00
abstract::During aetiological study of diseased red clover (Trifolium pratense L.) using high throughput sequencing, a novel virus with a 10 kb genome divided into two segments was discovered. The virus, tentatively named red clover associated varicosavirus (RCaVV), is phylogenetically related to classifiable members of the gen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3838-2
更新日期:2018-08-01 00:00:00