Abstract:
:The CPK cells derived from swine kidney were infected with the attenuated TO-163 strain of transmissible gastroenteritis (TGE) virus, and fused with uninfected Vero cells in the presence of polyethylene glycol. Repeated cocultivation of the fused cells with uninfected Vero cells rendered the virus to grow in Vero cells. The Vero cell-adapted virus acquired the ability to infect and produce cytopathic effects in several other non-permissive cell lines of non-porcine origin. No major differences in viral polypeptides were shown between the Vero cell-adapted TO-163 strain and its parent strain by indirect immunofluorescence and Western blotting using monoclonal and polyclonal antibodies to TGE virus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Ishii H,Watanabe I,Mukamoto M,Kobayashi Y,Kodama Ydoi
10.1007/BF01321128keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
201-6issue
1-2eissn
0304-8608issn
1432-8798journal_volume
122pub_type
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