Abstract：
:Chimeric plant viruses are emerging as promising vectors for use in innovative vaccination strategies. In this context, cucumber mosaic virus (CMV) has proven to be a suitable carrier of the hepatitis C virus (HCV)-derived R9 mimotope. In the present work, a new chimeric CMV, expressing on its surface the HCV-derived R10 mimotope, was produced but lost the insert after the first passage on tobacco. A comparative analysis between R10- and R9-CMV properties indicated that R9-CMV stability was related to structural features typical of the foreign insert. Thus, in order to combine high virus viability with strong immuno-stimulating activity, we doubled R9 copies on each of the 180 coat protein (CP) subunits of CMV. One of the chimeras produced by this approach (2R9-CMV) was shown to systemically infect the host, stably maintaining both inserts. Notably, it was strongly recognized by sera of HCV-infected patients and, as compared with R9-CMV, displayed an enhanced ability to stimulate lymphocyte IFN-gamma production. The high immunogen levels achievable in plants or fruits infected with 2R9-CMV suggest that this chimeric form of CMV may be useful in the development of oral vaccines against HCV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Nuzzaci M,Piazzolla G,Vitti A,Lapelosa M,Tortorella C,Stella I,Natilla A,Antonaci S,Piazzolla Pdoi
10.1007/s00705-006-0916-7subject
Has Abstractpub_date
2007-01-01 00:00:00pages
915-28issue
5eissn
0304-8608issn
1432-8798journal_volume
152pub_type
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