Abstract:
:The influenza season 2014/15 was dominated by drift variants of influenza A(H3N2), which resulted in a reduced vaccine effectiveness. It was not clear if the performance of commercial nucleic-acid-based amplification (NAT) assays for the detection of influenza was affected. The purpose of this study was to perform a real-life evaluation of two commercial NAT assays. During January-April 2015, we tested a total of 665 samples from patients with influenza-like illness using the Fast Track Diagnostics Respiratory pathogens 21, a commercial multiplex kit, (cohorts 1 and 2, n = 563 patients) and the Xpert Flu/RSV XC assay (cohort 3, n = 102 patients), a single-use cartridge system. An in-house influenza real-time RT-PCR (cohort 1) and the RealStar Influenza RT-PCR 1.0 Kit (cohort 2 and 3) served as reference tests. Compared to the reference assay, an overall agreement of 95.9 % (cohort 1), 95 % (cohort 2), and 98 % (cohort 3) was achieved. A total of 24 false-negative results were observed using the Fast Track Diagnostics Respiratory pathogens 21 kit. No false-negative results occurred using the Xpert Flu/RSV XC assay. The Fast Track Diagnostics Respiratory pathogens 21 kit and the Xpert Flu/RSV XC assay had sensitivities of 90.7 % and 100 % and specificities of 100 % and 94.1 %, respectively, compared to the RealStar 1.0 kit. Upon modification of the Fast Track Diagnostics Respiratory pathogens 21 kit, the sensitivity increased to 97.3 %. Influenza virus strains circulating during the 2014/15 season reduced the detection sensitivity of a commercial NAT assay, and continuous monitoring of test performance is therefore necessary.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Huzly D,Korn K,Bierbaum S,Eberle B,Falcone V,Knöll A,Steininger P,Panning Mdoi
10.1007/s00705-016-2930-8subject
Has Abstractpub_date
2016-09-01 00:00:00pages
2417-23issue
9eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-2930-8journal_volume
161pub_type
杂志文章abstract::Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously dete...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2217-x
更新日期:2015-01-01 00:00:00
abstract::TT virus (TTV), the first human circovirus to be discovered, appears to be present in most people; less is known about the prevalence of the related TTV-like mini virus (TLMV). A sensitive nested PCR, specific for TLMV, detected the virus in 48% of 201 sera (Norwegian blood donors) previously found to have a 90% preva...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s705-002-8310-6
更新日期:2002-01-01 00:00:00
abstract::To investigate the genetic diversity of potato virus M (PVM; genus Carlavirus, family Betaflexiviridae), the complete nucleotide sequence of the coat protein gene of 30 PVM isolates from a major potato-growing region in Iran were determined. Phylogenetic analysis of these Iranian PVM isolates together with those avail...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2037-z
更新日期:2014-08-01 00:00:00
abstract::A potyvirus isolated from Pinellia ternata in China was characterised and shown to be related to Soybean mosaic virus (SMV). The virus was pathogenic on P. ternata and some soybean cultivars, whereas the local soybean SMV isolate HH5 did not infect P. ternata. Western blot experiments demonstrated a serological relati...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0184-8
更新日期:2004-02-01 00:00:00
abstract::The PI3K/Akt signalling pathway is a crucial signalling cascade that regulates transcription, protein translation, cell growth, proliferation, cell survival, and metabolism. During viral infection, viruses exploit a variety of cellular pathways, including the well-known PI3K/Akt signalling pathway. Conversely, cells r...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-020-04740-1
更新日期:2020-10-01 00:00:00
abstract::While infectious bursal disease virus (IBDV) mainly targets immature B cells and causes T cell infiltration in the bursa of Fabricius (BF) of chickens, the effect of IBDV infection on the properties of T cells and relevant cytokine production in avian gut-associated lymphoid tissues (GALTs) remains unknown. Here, we s...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04735-y
更新日期:2020-10-01 00:00:00
abstract::Previously, we identified serological immunodeterminants of African swine fever virus (ASFV), including pK205R and pB602L, without homologues in the database. pK205R is expressed as a 33-kD protein from 4 h post-infection onward, initially diffusely distributed throughout cells, and subsequently in viral factories. pK...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0246-z
更新日期:2008-01-01 00:00:00
abstract::In previous work, we demonstrated that the arenavirus Junín virus (JUNV) is able to activate Akt by means of the phosphatidylinositol-3-kinase (PI3K) survival pathway during virus entry. This work extends our study, emphasizing the relevance of this pathway in the establishment and maintenance of persistent infection ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2298-6
更新日期:2015-02-01 00:00:00
abstract::Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3070-x
更新日期:2016-12-01 00:00:00
abstract::Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309576
更新日期:1992-01-01 00:00:00
abstract::Concentrations of DEAE-dextran which are currently used to stimulate the interaction between animal cells and natural or synthetic polynucleotides, inhibit protein synthesis in HeLa cells. This inhibition results from the blocking of more than one of the steps in protein synthesis, including transport of amino acids. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF02175599
更新日期:1975-01-01 00:00:00
abstract::A replication-defective, recombinant Sindbis virus vector was utilized in a novel immunization strategy to induce humoral and cellular responses against hepatitis C virus (HCV). The recombinant vector, pVaXJ-E1E2, expressing the gene for HCV glycoproteins E2 and E1, was constructed by inserting the E1E2 gene into the ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1564-8
更新日期:2013-05-01 00:00:00
abstract::DNA polymerase (DNApol) is present in all baculoviruses and plays a crucial role in viral DNA replication. Previously we showed that the DNApol of the alphabaculovirus group II Spodoptera litura nucleopolyhedrovirus (SpltNPV) could partially substitute for the DNApol of a group I alphabaculovirus, Autographa californi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3468-0
更新日期:2017-11-01 00:00:00
abstract::The highly conserved encapsidation signal (epsilon) of hepatitis B viral (HBV) pregenomic RNA has been reported as an essential component for encapsidation and protein priming of HBV polymerase. Here, we report that two HBV epsilon RNA-binding host proteins (80 and 43 kDa) and a copurifying protein (100 kDa) were puri...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050200001
更新日期:2002-03-01 00:00:00
abstract::For selecting the neutralizing monoclonal antibodies (N-MAbs) directed to VP4 of rotavirus strain K8, which has unique VP4 neutralization epitopes, we prepared several reassortant viruses by mixed infection of two different strains K8 (serotype 1) and P (serotype 3) in vitro: three reassortant clones having VP4 of K8 ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316751
更新日期:1991-01-01 00:00:00
abstract::Recently we have described that the Herpes simplex virus (HSV)-induced thymidine kinase (TK) induces AMP- and ADP-dThd-5'-phosphotransferase activities. We now demonstrate the heterogeneity of the described activities in isoelectric focusing experiments and polyacrylamide gel electrophoresis. A TK--mutant of HSV type ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314875
更新日期:1982-01-01 00:00:00
abstract::Poliovirus induces bcl-2-independent apoptosis in the human U937 promonocytic cell line [28]. Here we describe that this cell death, induced after viral infection, correlates with the modulation of the protooncoprotein Bcl-xl. Furthermore, poliovirus infection decreases the detected Bcl-xl in a U937 clone that overexp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0881-8
更新日期:2002-12-01 00:00:00
abstract::Since late 2018, foot-and-mouth disease (FMD) has reemerged and rapidly swept through pig farms in North and Central Vietnam, despite widespread use of commercial FMD vaccines. To investigate the FMD virus (FMDV) strains responsible for the current epidemics, 40 FMDV samples were collected from 17 provinces during Nov...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04763-8
更新日期:2020-11-01 00:00:00
abstract::Genetic relationships between the genome segments of six cypovirus (CPV) isolates were analysed by RNA cross-hybridisation. These included three type 1 viruses and single isolates of types 2, 5 and 12, which collectively are identical to those previously compared by serology and electrophoresis [Mertens et al. (1989),...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050525
更新日期:1999-01-01 00:00:00
abstract::Here, we present the complete genomic sequence of duck enteritis virus (DEV) strain SD, isolated in China in 2012. The virus was virulent in experimentally infected 2-month-old ducks. The DEV SD genome is 160,945 base pairs (bp) in length. The viral genome sequence, when compared to that of strain DEV CSC, which was i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04594-7
更新日期:2020-07-01 00:00:00
abstract::Immunoblot analysis of apple latent spherical cheravirus (ALSV)-infected leaves using a polyclonal antibody against the 21 C-terminal amino acids of a 53 K/42 K movement protein (MP) showed that a protein with an Mr of 42 kDa (42KP) is the dominant form found in vivo, which could indicate that the second AUG is used a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0689-4
更新日期:2006-05-01 00:00:00
abstract::A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck, and goose reovirus (ARV, DRV, and GRV) RNA from cell culture supernatant and clinical samples was established. Based on multiple sequence alignment, a pair of degenerate primers was selected and synthesized. The ampl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0731-1
更新日期:2006-08-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) causes a variety of cancers, including Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman disease (MCD). Host cellular microRNAs (miRNAs) play important post-transcriptional regulatory roles in gene expression and can greatly influence viru...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3478-y
更新日期:2017-11-01 00:00:00
abstract::In cultured chicken embryo fibroblasts treated with copper-(copper cation 10-minus 3.5-10-minus 5 M), nickel and cobalt salts an enhancement of the plating efficiency of Semliki Forest virus could be observed. This augmented plaque formation was most probably due to a higher adsorption rate of virions to the cell surf...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315593
更新日期:1975-01-01 00:00:00
abstract::The gene coding for the nucleocapsid protein NP of the influenza A virus recombinant strain 413 1,1 was characterized biochemically by molecular hybridization and fingerprint analysis. The data presented suggest that this NP gene has evolved by intracistronic recombination between NP genes of virus N and the fowl plag...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322701
更新日期:1980-01-01 00:00:00
abstract::Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HER...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0764-5
更新日期:2006-10-01 00:00:00
abstract::Each of 20 gilts (principals) from a commercial swine herd free of antibody for porcine parvovirus (PPV) was exposed intranasally and orally to PPV at the onset of gestation. The gilts were killed and necropsied 22 +/- 1 days later to determine the effect of the virus on their embryos. An equal number of gilts (contro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01340540
更新日期:1980-01-01 00:00:00
abstract::The adsorption and retention properties of native (unfixed) and glutaraldehyde-fixed alfalfa mosaic virus (AMV) antigens to the polystyrene of ELISA plates were studied using [35S]-labelled virus preparations. It was shown that adsorption was a temperature-dependent, relatively slow process which varied between differ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310767
更新日期:1991-01-01 00:00:00
abstract::The amino acid sequences of the regions encoding the structural proteins of eleven nepoviruses and five comoviruses, two genera of the family Comoviridae, have been aligned. The properties predicted by computer analysis (three-dimensional-3D-structure, hydrophobicity) are also correlated along this alignment, and alig...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322691
更新日期:1995-01-01 00:00:00
abstract::Treatment of Cricket paralysis virus infected Drosophila cells with iodoacetamide before radiolabelling with 35S-methionine results in the appearance of two high molecular weight polypeptides of approximately equal to 200,000 molecular weight, not apparent in untreated infected cells (17). To attempt to differentiate ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320788
更新日期:1981-01-01 00:00:00