Abstract：
:Treatment of Cricket paralysis virus infected Drosophila cells with iodoacetamide before radiolabelling with 35S-methionine results in the appearance of two high molecular weight polypeptides of approximately equal to 200,000 molecular weight, not apparent in untreated infected cells (17). To attempt to differentiate between the effects of iodoacetamide being attributable to either alteration of initial polyprotein or inhibition of the protease (either cellular or viral) the effects of a spectrum of protease inhibitors were examined. These included aprotinin, leupeptin, pepstatin, elevated zinc concentration, phenyl methyl sulphonyl-fluoride, N-tosyl-L-lysine chloromethyl ketone (TLCK) and N-tosyl-L-phenylalanine chloromethyl ketone (TPCK). TLCK and TPCK both inhibited the cleavage of proteins which demonstrates an inhibition of the protease activity. The introduction of amino acid analogues into the infected cells before pulsing also results in the appearance of higher molecular weight proteins. This could be attributed to alternation of the polyprotein making it nonsusceptible to digestion with pre-existing cellular protease or newly synthesized viral protease. The possibility that the presence of the amino acid analogues results in alteration of a viral coded protease cannot be eliminated.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Moore NF,Pullin JS,Reavy Bdoi
10.1007/BF01320788subject
Has Abstractpub_date
1981-01-01 00:00:00pages
1-9issue
1eissn
0304-8608issn
1432-8798journal_volume
70pub_type
杂志文章abstract：:The hemagglutinin-esterase (HE) protein of betacoronavirus lineage A is a secondary receptor in the infection process and is involved in the emergence of new betacoronavirus genotypes with altered host specificity and tissue tropism. We previously reported a novel recombinant bovine coronavirus (BCoV) strain that was ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04840-y
更新日期：2020-12-01 00:00:00
abstract：:The complete sequence of 8159 nucleotides of the double stranded DNA genome of cassava vein mosaic virus (CsVMV) was determined (# U59751) and revealed a significant difference in genome organization when compared with a previous report (# U20341). When transferred to cassava plants by microbombardment, the full lengt...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050344
更新日期：1998-01-01 00:00:00
abstract：:Using monoclonal antibodies raised against a Faba bean necrotic yellows virus (FBNYV) isolate from Egypt and a Faba bean necrotic stunt virus (FBNSV) isolate from Ethiopia, a striking serological variability among nanovirus isolates from faba bean in Morocco was revealed. To obtain a better understanding of this nanov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0548-9
更新日期：2010-01-01 00:00:00
abstract：:Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy cal...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04792-3
更新日期：2020-11-01 00:00:00
abstract：:Two types of transcripts, incomplete (polyadenylated virus-specific mRNAs) and complete (presumably templates for progeny virus replication) are synthesized in influenza virus infected cells (3). The synthesis of incomplete transcripts requires as a primer de novo synthesized host cell mRNAs (4). We show here that the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314609
更新日期：1981-01-01 00:00:00
abstract：:Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2605-x
更新日期：2015-12-01 00:00:00
abstract：:Virological surveillance is an important element in the Polio Eradication Initiative to provide information rapidly about circulating wild polioviruses. Molecular tools have been developed to identify the serotype of the poliovirus strains and whether they are of vaccine or wild origin (intratypic differentiation) and...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0001-x
更新日期：2008-01-01 00:00:00
abstract：:This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1673-z
更新日期：2013-08-01 00:00:00
abstract：:Drosophila C virus RNA acted as mRNA in rabbit reticulocyte lysates and directed the synthesis of at least one capsid protein and a number of higher molecular weight proteins. Kinetic analysis by pulse-chase experiments showed that a number of high molecular weight products acted as precursors to the capsid protein(s)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311694
更新日期：1983-01-01 00:00:00
abstract：:A hemagglutinating virus (8KS0813) was isolated from a red-necked stint. Hemagglutination inhibition and neutralization tests indicated that 8KS0813 was antigenically related to a prototype strain, APMV-6/duck/Hong Kong/18/199/77, but with an 8- and 16-fold difference, respectively, in their titers. The full genome se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2162-8
更新日期：2014-11-01 00:00:00
abstract：:We constructed three sub-genomic replicons of Tick-borne encephalitis virus (TBEV) (Oshima REP, Oshima REP-GFP and Oshima REP-Neo) by deleting genes coding for structural proteins without or with insertion of green fluorescent protein (GFP) or Neo genes, respectively. BHK cells transfected with Oshima REP expressed th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0262-y
更新日期：2004-06-01 00:00:00
abstract：:Type I interferon (IFN) exerts strong antiviral activity, particularly against human immunodeficiency virus (HIV), and although several viral proteins have been shown to deregulate IFN induction, little is known about the induction of type I IFNs by HIV RNAs. In the present study, we used the stable isotope labeling w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2372-8
更新日期：2015-05-01 00:00:00
abstract：:A rotavirus-like virus has been isolated from cases of neonatal diarrhoea in piglets. No antigenic relationship with known rotaviruses or with an American strain of a rotavirus-like virus has been demonstrated. Morphologically the virus is similar to known rotaviruses, but it differs in the ability to form syncytia of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311220
更新日期：1984-01-01 00:00:00
abstract：:The thymidine kinase (TK) of herpesviruses, in contrast to cellular TKs, phosphorylates a variety of substrates including antiherpetic nucleoside analogues. This study reports the identification and DNA sequence of the simian varicella virus (SVV) TK gene. A 32P-labeled varicella zoster virus (VZV) TK DNA probe hybrid...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718587
更新日期：1996-01-01 00:00:00
abstract：:Interleukin-2 dependent feline T-lymphoblastoid cells designated as MYA-1 cells were established. The cells were free from exogenous retroviruses and sensitive for replication of feline immunodeficiency virus (FIV). FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313750
更新日期：1989-01-01 00:00:00
abstract：:Deep sequencing of small RNA (sRNA) populations in maize plants from southwest China resulted in the identification of a previously unknown dsRNA virus with a sequence and genome organization resembling that of a totivirus. The complete viral genome is 3,956 nucleotides in length and contains two open reading frames (...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2657-y
更新日期：2016-02-01 00:00:00
abstract：:Hippeastrum chlorotic ringspot virus (HCRV) is a novel tospovirus that was identified in Yunnan Province, China, in 2013. We have sequenced the HCRV L gene, which is 8909 nt long and encodes the RNA-dependent RNA polymerase (2873 amino acids, 330.8 kDa). The HCRV L protein shared highest similarity (89.4 %) with that ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2100-9
更新日期：2014-10-01 00:00:00
abstract：:Respiratory syncytial virus (RSV) is the most frequently identified viral agent in infants, children, and elderly people with acute respiratory tract infections (ARTIs). This study is the only one of its kind in Kuwait, and its purpose was to investigate the genetic variability of the G protein gene in RSV strains pre...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3881-z
更新日期：2018-09-01 00:00:00
abstract：:Human adenoviruses (HAdV) of species B, C, and E (HAdV-B, -C, -E) are frequent causative agents of acute respiratory infections worldwide. No specific analysis has been done on the epidemiological and clinical features of HAdV in pediatric pneumonia in China. Nasopharyngeal aspirates were collected from hospitalized c...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2308-8
更新日期：2015-03-01 00:00:00
abstract：:The interaction between two different bovine respiratory syncytial virus (BRSV) strains and bovine alveolar macrophages (BAMs) was studied in vitro. Bovine respiratory syncytial virus replicated minimally in BAMs and most of the virus produced remained cell-associated. Approximately 1 out of 1,000 BAMs produced infect...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322681
更新日期：1995-01-01 00:00:00
abstract：:To characterize the role of the humoral immune response on HIV-1 infection of monocytes and macrophages (M phi s) we examined the susceptibility of in vitro cultured monocyte/M phi s to various HIV-1 isolates and the influence of heterologous and particularly autologous anti HIV-1 sera on this infection. Depending on ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01538815
更新日期：1994-01-01 00:00:00
abstract：:Subnormal temperatures were found to depress the production of interferon by bovid herpesvirus 2 (BHV-2)-infected bovine testicular cells, bovine peripheral blood leukocytes, and bovine monocytes, as well as by BHV-2 antigen-stimulated immune peripheral blood leukocytes. Interferon titers generated at 30 degrees C wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311164
更新日期：1984-01-01 00:00:00
abstract：:Virazole (1, beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide = Ribavirin), a synthetic nucleoside analogue, has been successfully used against tomato spotted wilt virus (TSWV) in tomato and tobacco plants. In tomato, the most efficient concentration to control TSWW was 500 mg/l while in tobacco, concentrations of 10...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315037
更新日期：1980-01-01 00:00:00
abstract：:Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for the presence of anti-aHEV antibodies. In a random serology study...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4089-y
更新日期：2019-02-01 00:00:00
abstract：:We determined the complete nucleotide sequence of EDRD-1, a Japanese strain of the North American type-Porcine reproductive and respiratory syndrome virus (PRRSV), and identified a novel 117-base deletion and 108-base insertion previously reported in the nsp2 gene of the SP strain, which contains the largest genome am...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0098-6
更新日期：2008-01-01 00:00:00
abstract：:Electropherotypes (ET), serotypes, and subgroups of equine rotaviruses isolated from foals in Japan were determined. The ETs of 136 isolates from 1981 through to 1991 were divided into six groups: ET-A-ET-F. The ET-A, -B, -C, -D, -E, and -F were present in 3, 1, 121, 9, 1, and 1 strains, respectively. Representative v...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379088
更新日期：1993-01-01 00:00:00
abstract：:The first sporadic cases of Fujian/411/02-lineage viruses were recorded in Finland in winter 2001-2002. The first protracted but low-intensity outbreak occurred here during the first half of 2003, and the second outbreak early in autumn 2003, after detection of sporadic influenza A cases in the summer. The calculated ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0635-5
更新日期：2006-02-01 00:00:00
abstract：:Here, we report the molecular characterization of a novel partitivirus from Phomopsis vexans strain PvHZ002, a plant-pathogenic fungus infecting eggplant. The virus was designated "Phomopsis vexans partitivirus 1" (PvPV1). PvPV1 contains two dsRNA segments, dsRNA1 and dsRNA2, which are 1,662 bp and 1,628 bp long, resp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04835-9
更新日期：2021-01-01 00:00:00
abstract：:Truncated alpha 4 genes were introduced into BHK tk- cells along with the neomycin phosphotransferase gene, that confers resistance to the eukaryotic antibiotic G418, driven by the HSV-1 beta tk promoter (beta tk- neor). Stably transformed cell lines were obtained and studied for the ability of the resident truncated ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316907
更新日期：1993-01-01 00:00:00
abstract：:In addition to acute infection and disease, foot-and-mouth disease virus (FMDV) can cause persistent infection in ruminants. Such "carrier" animals represent a potential risk for FMDV transmission to susceptible animals. However, the mechanisms and the factors that determine FMDV persistence remain unknown. We describ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2526-8
更新日期：2015-10-01 00:00:00
