Cassava vein mosaic virus (CsVMV), type species for a new genus of plant double stranded DNA viruses?

Abstract:

:The complete sequence of 8159 nucleotides of the double stranded DNA genome of cassava vein mosaic virus (CsVMV) was determined (# U59751) and revealed a significant difference in genome organization when compared with a previous report (# U20341). When transferred to cassava plants by microbombardment, the full length CsVMV clone was infectious, confirming the genome organization here described. Sequence comparisons between CsVMV and members of the genera Caulimovirus and Badnavirus revealed high homologies between consensus sequences of several proteins that are indispensable for virus replication, including a potential transactivator factor not reported previously. The presence of a sequence complementary to a plant Met tRNA confirms that CsVMV is a plant pararetrovirus and is most closely related to members of the genus Caulimovirus as previously assessed. However, differences in genome organization, number and size of the ORFs, in addition to sequence comparisons with other plant pararetroviruses, shows that either the genetic variability of caulimoviruses is much greater than previously thought, or that CsVMV is the unique representative of a new genus within the Caulimoviridae family. On the basis of this study, it is proposed to upgrade the floating genus Caulimovirus to the family level and to divide the Caulimoviridae family into at least three genera with CsVMV being the type member of a new genus.

journal_name

Arch Virol

journal_title

Archives of virology

authors

de Kochko A,Verdaguer B,Taylor N,Carcamo R,Beachy RN,Fauquet C

doi

10.1007/s007050050344

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

945-62

issue

5

eissn

0304-8608

issn

1432-8798

journal_volume

143

pub_type

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