Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method.

Abstract:

:The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strain. These have been used to produce full-length DNA copies of the two genomes each within a single plasmid.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Stanway G,Mountford RC,Cox SD,Schild GC,Minor PD,Almond JW

doi

10.1007/BF01309297

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

67-78

issue

1-2

eissn

0304-8608

issn

1432-8798

journal_volume

81

pub_type

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