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Purification of mengovirus by freon extraction and chromatography on protein-coated controlled pore glass.

Abstract：

:Mengovirus, extracted from infected L-cell cultures with Freon 113 and concentrated from the aqueous phase with polyethyleneglycol, was chromatographed on protein-coated controlled pore glass (CPG). The covalent binding of protein to CPG is described. Further purification and concentration of mengovirus was achieved by isopycnic density gradient centrifugation in solutions of either CsCl or salts of iodinated benzoic acid derivatives. The described procedure is superior to conventional methods for the isolation and purification of large quantities of mengovirus. It yields highly purified virus preparations within a short time and a recovery of more than 50% of the starting infectivity.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Gschwender HH,Traub P

doi

10.1007/BF01315283

subject

Has Abstract

pub_date

1978-01-01 00:00:00

pages

327-36

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

56

pub_type

杂志文章

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