Abstract:
:Cocksfoot mottle sobemovirus (CfMV) has a monopartite single-stranded positive-sense RNA genome. In wheat germ extract, in vitro translation of CfMV virion RNA resulted in the production of four major proteins of 100, 71, 34 and 12 kDa. In this paper we show the individual genes from which these polypeptides are synthesised. Polyclonal antisera against the proteins encoded by all open reading frames (ORFs) of CfMV were produced. Antibodies were used to immunoprecipitate the in vitro translation products of CfMV genomic RNA as well as the coupled in vitro transcription/translation products of individual viral genes. We demonstrate that the 12, 71, and 100 kDa CfMV proteins are synthesised from the bicistronic genomic RNA of the virus. The CfMV 12 kDa protein is produced from ORF1, the 71 kDa protein from ORF2a (and does not result from the proteolytic cleavage of the polyprotein) and the 100 kDa protein is a polyprotein encoded by ORFs 2a and 2b by a -1 ribosomal frameshift. ORF2b alone does not direct any in vitro protein synthesis. CfMV 34 kDa protein is a coat protein synthesised from the virion-packed subgenomic RNA. The translational strategies of different sobemoviruses are also discussed in this paper.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Tamm T,Mäkinen K,Truve Edoi
10.1007/s007050050610keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
1557-67issue
8eissn
0304-8608issn
1432-8798pii
91441557.705journal_volume
144pub_type
杂志文章abstract::The duration of infection with rat virus (RV), an autonomous rodent parvovirus, was examined at multiple intervals over 6 months in rats inoculated by the oronasal route at 2 days of age or 4 weeks of age and individually housed after weaning to prevent cross-infection. Infectious virus was recovered by explant cultur...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310765
更新日期:1991-01-01 00:00:00
abstract::Reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the rapid laboratory diagnosis of pestivirus infections. A direct DNA sequencing method was developed for the analysis of the amplified cDNA from the 5' noncoding region of the viral genome. 70 pestivirus strains were compared in th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309467
更新日期:1994-01-01 00:00:00
abstract::Synthetic peptides comprising the predicted principal neutralizing determinant (PND) in new African and North American HIV-1 clones were tested in ELISA for reactivity with ninety six serum samples from asymptomatic donors in six selected localities in Uganda. Irrespective of the geographical origin of the samples, th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322666
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:In May 2018, a 8 year old thoroughbred mare died at an equestrian club in Changji, Xinjiang, China. The horse had been imported from the United States in 2013. She became pregnant in December 2016 but, after foaling, gradually lost weight and died in May 2018. This study aim to identify the pathogen, who cau...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04487-4
更新日期:2020-02-01 00:00:00
abstract::Hemorrhagic fever with renal syndrome (HFRS) is endemic in East Asia and Europe. The disease is caused by several viruses belonging to the genus Hantavirus, including the Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava Belgrade virus (DOBV), and Puumala virus (PUUV). Recently, HTNV-related viruses, Amur (AMR) and Fa...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0113-x
更新日期:2003-08-01 00:00:00
abstract::Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reacti...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3859-x
更新日期:2018-09-01 00:00:00
abstract::The structure and organization of the large (L) RNA of iris yellow spot virus (IYSV) was determined, and with this report, the complete genomic sequence of IYSV of the genus Tospovirus, family Bunyaviridae has been elucidated. The L RNA of IYSV was 8,880 nucleotides in length and contained a single open reading frame ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0568-5
更新日期:2010-02-01 00:00:00
abstract::Serotype G14 was once considered to be uncommon among equine rotaviruses. While it sporadically emerged in some parts of the world, serotype G14 became the dominant G serotype among rotaviruses detected in foals with diarrhea in Japan in the late 1990s. However, it is not known how such recently emerging G14 rotavirus...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0967-3
更新日期:2003-05-01 00:00:00
abstract::Chikungunya fever is self-limiting. However, neurological and hemorrhagic complications have been seen in recent outbreaks. The clinical manifestations of this disease are similar to those of dengue virus infection, indicating the need for differential diagnosis in areas such as India, which are endemic for both virus...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2829-4
更新日期:2016-06-01 00:00:00
abstract::Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3618-4
更新日期:2018-02-01 00:00:00
abstract::Complete genome sequences of two new isolates of narcissus late season yellows virus (NLSYV) from Australia were compared with the other NLSYV genome from China and with two complete genomes of isolates designated narcissus yellow stripe virus (NYSV), one from Australia and the other from China. On the basis of sympto...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1969-z
更新日期:2014-06-01 00:00:00
abstract::Human astrovirus (HAstV) infections were confirmed in 17 (1.5%) of 1153 children and adults with diarrhea, from different regions of Korea, by testing their stool samples by reverse transcription-polymerase chain reaction using primers targeted to partial ORF 1a. Genotypes of isolates were determined by sequencing the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0844-0
更新日期:2002-09-01 00:00:00
abstract::The pathogenicity of 5 different encephalomyocarditis (EMC) virus isolates was investigated in swine fetuses following injection of each virus in utero. Laparotomies were performed on 3 pregnant sows in early mid-third (39-40 days) of gestation and on 5 sows in the late mid-third (70-72 days) of gestation, and groups ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310517
更新日期:1989-01-01 00:00:00
abstract::There were three epidemic waves of human infection with avian influenza A (H7N9) virus in 2013-2014. While many analyses of the genomic origin, evolution, and molecular characteristics of the influenza A (H7N9) virus have been performed using sequences from the first epidemic wave, genomic characterization of the viru...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2872-1
更新日期:2016-08-01 00:00:00
abstract::Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously dete...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2217-x
更新日期:2015-01-01 00:00:00
abstract::Molecular analyses of viruses infecting Chinese scallion (Allium chinense G. Don) showed that the plants did not contain any of the poty-, carla- or allexiviruses that are common in garlic plants in China. The complete sequences of a potyvirus and a potexvirus were determined and these were shown to represent differen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050200018
更新日期:2002-04-01 00:00:00
abstract::Complementary oligonucleotide primers which flank a 1146-nucleotide gene fragment (S1B: nt 1185 to 2333) encompassing a polymorphic region (nt 1368 to 1776) of the S1 subunit of bovine coronavirus spike glycoprotein were used for enzymatic amplification by PCR. We chose four clinical isolates, recovered from cases of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310017
更新日期:1994-01-01 00:00:00
abstract::Yellow mosaic disease was observed on Jatropha gossypifolia plants growing in Kathaupahadi, Madhya Pradesh, India, and whiteflies (Bemisia tabaci) were found in the vicinity. Association of a new begomovirus with yellow mosaic disease of J. gossypifolia has been detected by PCR using begomovirus DNA-A-specific primers...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1118-5
更新日期:2011-12-01 00:00:00
abstract::The rate of evolution of the human papillomavirus 16 (HPV16) genome is low. However, the ability of the E6 oncoprotein to interact with distinct p53 variants causes selective pressure on the E6 gene. In addition, intratypic recombination events in the HPV16 E6 and E7 genes have been characterized as extraordinary phen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1555-9
更新日期:2013-04-01 00:00:00
abstract::The V3 domain is highly variable and induces HIV neutralizing antibodies (NA). Here we addressed the issues of 1) the participation of mutations in V3 in generation of neutralization resistant escape virus in vivo and 2) the applicability of synthetic V3 peptides corresponding to field isolates to induce neutralizing ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309956
更新日期:1995-01-01 00:00:00
abstract::Scrapie and Creutzfeldt-Jakob disease (CJD) are caused by infectious agents that are defined phenomenologically. No agent-specific molecules or particles have been identified. Biological properties, such as exponential agent replication and strain variation, as well as physical characteristics of infectivity indicate ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309662
更新日期:1993-01-01 00:00:00
abstract::The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degree...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309574
更新日期:1992-01-01 00:00:00
abstract::Viral infections of the central nervous system (CNS) following peripheral inoculation of Sindbis viruses were studied. The use of viral strains, which vary in their neuroinvasive and neurovirulent properties, and various strains of mice, which differ in immunocompetence, revealed several pathways of viral neuroinvasio...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050576
更新日期:1999-01-01 00:00:00
abstract::Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310491
更新日期:1991-01-01 00:00:00
abstract::The ability of lymphocytes from patients with multiple sclerosis (MS) to produce Interferon (IFN-alpha) in response to Newcastle Disease Virus (NDV) was studied in vitro. The correlation between individual IFN-alpha titers and natural killer (NK) cell activity and the presence of HLA system antigens associated with MS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314877
更新日期:1982-01-01 00:00:00
abstract::Echovirus 33 (E33) has been infrequently detected and is less frequently associated with clinical diseases when compared with other types of enteroviruses (EVs) in China. An outbreak of E33 was identified in four schools in Hunan Province, China, in June 2013. For laboratory diagnosis, throat swabs and/or serum specim...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2059-6
更新日期:2014-09-01 00:00:00
abstract::Tryptic peptide analyses were performed on the major internal structural proteins of type D retroviruses isolated originally from subhuman primate species (MPMV, SMRV, LV) and from permanent human cell lines (HeLaV, HEp-2V, PMFV). The p25 peptide maps of MPMV, LV, PMFV and HEp-2V were very similar but showed a strikin...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309302
更新日期:1984-01-01 00:00:00
abstract::Enzootic bovine leukosis (EBL) is a malignant B-cell lymphoma of cattle caused by infection with bovine leukemia virus (BLV). It is defined by clonal and neoplastic expansion of BLV-infected B cells. Currently, multiple examinations are able to comprehensively diagnose this condition. Inverse polymerase chain reaction...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04945-4
更新日期:2021-01-24 00:00:00
abstract::Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314653
更新日期:1988-01-01 00:00:00
abstract::Electropherotypes (ET), serotypes, and subgroups of equine rotaviruses isolated from foals in Japan were determined. The ETs of 136 isolates from 1981 through to 1991 were divided into six groups: ET-A-ET-F. The ET-A, -B, -C, -D, -E, and -F were present in 3, 1, 121, 9, 1, and 1 strains, respectively. Representative v...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379088
更新日期:1993-01-01 00:00:00