Abstract：
:Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, and p15 regions), pol (protease, reverse transcriptase, and endonuclease domains), env (gp120 region), tat, or rev were infectious, whereas vif, vpr, vpu, some of env (gp41) and nef mutants could grow in human CD4+ cells to various degrees. Of the non-infectious mutants, only endonuclease (pol) and gp41 mutants exhibited normal phenotypes with respect to the production of functional reverse transcriptase, the expression of gag, pol, and env proteins, and the generation of progeny virions, when examined in transient assays. All infectious mutants killed the CD4+ cells with the exception of a mutant carrying a defect in the vif gene.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Adachi A,Ono N,Sakai H,Ogawa K,Shibata R,Kiyomasu T,Masuike H,Ueda Sdoi
10.1007/BF01310491subject
Has Abstractpub_date
1991-01-01 00:00:00pages
45-58issue
1-2eissn
0304-8608issn
1432-8798journal_volume
117pub_type
杂志文章abstract：:We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embry...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379113
更新日期：1994-01-01 00:00:00
abstract：:Purified DNA from 45 isolates of suis herpes virus 1 (SHV1) collected between 1980 and 1987 from clinical outbreaks of Aujeszky's disease on French farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, confirming RFP analysis as a potential epidem...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01323161
更新日期：1990-01-01 00:00:00
abstract：:Seven putative mitoviral genomes, representing four species from three Sclerotinia sclerotiorum isolates, were fully sequenced. The genome lengths ranged from 2438 to 2815 nucleotides. The RNA-dependent RNA polymerase (RdRp) of one genome shared high amino acid (aa) sequence identity (98.5 %) with the previously descr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2171-7
更新日期：2014-11-01 00:00:00
abstract：:Current subgenotype 2.1 isolates of classical swine fever virus (CSFV) play a dominant role in CSF outbreaks in China, and a novel sub-subgenotype 2.1g of CSFV was recently identified, but the complete genome sequence of this new sub-subgenotype has not been reported. In this study, complete genome of 2.1g isolate GD1...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2932-6
更新日期：2016-09-01 00:00:00
abstract：:A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313815
更新日期：1989-01-01 00:00:00
abstract：:Two new isolates of cricket paralysis virus, TAR and SIM, are described that were originally isolated from laboratory colonies of Drosophila melanogaster and Drosophila simulans respectively. Using a combination of biological, serological and molecular characters it was possible to distinguish the SIM isolate from all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718251
更新日期：1996-01-01 00:00:00
abstract：:Interleukin-2 dependent feline T-lymphoblastoid cells designated as MYA-1 cells were established. The cells were free from exogenous retroviruses and sensitive for replication of feline immunodeficiency virus (FIV). FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313750
更新日期：1989-01-01 00:00:00
abstract：:It has been suggested that antibody overproduction plays a role in the pathogenesis of feline infectious peritonitis (FIP). However, only a few studies on the B-cell activation mechanism after FIP virus (FIPV) infection have been reported. The present study shows that: (1) the ratio of peripheral blood sIg(+) CD21(-) ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0265-9
更新日期：2009-01-01 00:00:00
abstract：:The resistance of transgenic Nicotiana benthamiana plants encoding wild type, truncated and point mutants of the tobacco etch virus (TEV) coat protein (CP) was analyzed. After R1 plants from 45 transgenic lines were challenged with TEV, six percent of the lines exhibited high resistance, 38% exhibited low resistance, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0577-y
更新日期：2005-12-01 00:00:00
abstract：:To trace the possible route of introduction of porcine epidemic diarrhea virus (PEDV), the phylogenetic relationships of PEDV strains in the regions of epidemicity in the Philippines to PEDV strains that are endemic in other countries were investigated. Partial nucleotide sequences of the S1 spike gene was determined ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2938-0
更新日期：2016-09-01 00:00:00
abstract：:An immune adherence hemagglutination test was established for detection of antibodies to respiratory syncytial virus. The test was shown to be as specific as and much more sensitive than the neutralization and complement fixation tests. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309371
更新日期：1984-01-01 00:00:00
abstract：:cDNA clones spanning the entire region of the peplomer (S) gene of feline infectious peritonitis virus (FIPV) type I strain KU-2 were obtained and their complete nucleotide sequences were determined. A long open reading frame (ORF) encoding 1464 amino acid residues was found in the gene, which was 12 residues longer t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718424
更新日期：1995-01-01 00:00:00
abstract：:In this study, a novel mycovirus designed Colletotrichum gloeosporioides ourmia-like virus 1 (CgOLV1) was isolated from a filamentous phytopathogenic fungus, Colletotrichum gloeosporioides. The virus has a genome of 2,516 nucleotides and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymeras...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04346-2
更新日期：2019-10-01 00:00:00
abstract：:Although viral protein 3 (VP3) of chicken anaemia virus (CAV) has been well recognised as an inducer of apoptosis, viral protein 2 (VP2) of the virus has only been speculated to have apoptotic activity. This has not been verified because the open reading frame (ORF) encoding VP2 completely encompasses that encoding VP...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2542-8
更新日期：2015-10-01 00:00:00
abstract：:Beak and feather disease virus (BFDV) is an avian circovirus, and it has a single-stranded DNA genome. It causes a fatal disease in parrots called psittacine beak and feather disease (PBFD). After screening of samples collected from Taiwan using PCR, complete genome sequences of isolates from 21 samples from various s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2963-z
更新日期：2016-11-01 00:00:00
abstract：:Chilo iridescent virus (CIV) belongs to the family Iridoviridae, which are icosahedral cytoplasmic DNA viruses with large, linear, and circularly permuted genomes. Previous studies on infected-cell-specific polypeptides suggested temporal regulation of CIV gene expression. Recently, we demonstrated three temporal clas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0232-4
更新日期：2004-04-01 00:00:00
abstract：:Aphidicolin, a specific inhibitor of cellular DNA polymerase alpha and of viral DNA polymerase, inhibits production of infectious virus and cellular and viral DNA synthesis of human cytomegalovirus (HCMV)-infected cells. On the other hand, 2',3'-dideoxynucleosides, inhibitors of DNA polymerases beta and gamma, do not ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310559
更新日期：1985-01-01 00:00:00
abstract：:A collection of 148 adenovirus strains of subgenus C collected in Argentina, Chile, and Uruguay were studied by restriction enzyme analysis of genomic DNA with endonucleases BamHI, BglII, BstEII, EcoRI, HindIII, KpnI, and SmaI. Only strains corresponding to serotypes 1, 2, and 5 were identified representing both alrea...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309841
更新日期：1993-01-01 00:00:00
abstract：:Genetically engineered expression of replicase encoding sequences has been proposed as an efficient system to confer protection against virus diseases by eliciting protection mechanisms in the plant. Potato leaf-roll was one of the first diseases for which this kind of protection was engineered in potato plants. Howev...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170095
更新日期：2001-07-01 00:00:00
abstract：:Recently, five new viruses from the genus Vitivirus were identified and named grapevine virus G, H, I, J and L. These viruses were targeted in a survey to evaluate their prevalence in different grapevine populations in California. Excluding a single detection of GVJ, other vitiviruses were detected infecting several g...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04355-1
更新日期：2019-10-01 00:00:00
abstract：:Genomic characterization of Norwalk-like human caliciviruses (NLVs) originating from outbreaks and sporadic cases of acute gastroenteritis has revealed surprisingly high levels of diversity, even in the RNA polymerase gene, which is anticipated to be highly conserved. Since information on antigenic relationship is lim...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050020
更新日期：2000-01-01 00:00:00
abstract：:Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this populati...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3224-5
更新日期：2017-06-01 00:00:00
abstract：:Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3788-8
更新日期：2018-07-01 00:00:00
abstract：:Chilo iridescent virus (CIV; the type strain of the genus Iridovirus) replicates productively in larvae of the boll weevil, Anthonomus grandis. This study focuses on characterizing productive infections of a boll weevil cell line, BRL-AG-3A (AG3A), starting with CIV reared in the waxworm, Galleria mellonella. We show ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1417-5
更新日期：2012-11-01 00:00:00
abstract：:An assay to characterize plasma human immunodeficiency virus 1 (HIV-1) sequences for patients with low viral loads was developed by combining the selective binding of anti-CD44 MicroBeads with a nested RT-PCR targeting the env C2V4 region. Sequences were obtained from 10 of 20 HIV+ patients who had viral loads below 4...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0659-3
更新日期：2010-06-01 00:00:00
abstract：:Gnotobiotic lambs were protected against rotavirus infection by the presence in the gut at the time of infection of colostrum or serum containing antibodies to rotavirus. This protection was observed even when passively-acquired antibody was not present in the serum of the infected lamb. Infection under these conditio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01348017
更新日期：1976-01-01 00:00:00
abstract：:Styrian oil pumpkin seedlings (Cucurbita pepo L. subsp. pepo var. styriaca GREB: .) were treated for 48 h with 1 mM OTC (L-2-oxothiazolidine-4-carboxylic acid) in order to artificially increase cellular glutathione content. They were inoculated with zucchini yellow mosaic virus (ZYMV) 10 days later. The effects of OTC...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0880-2
更新日期：2007-01-01 00:00:00
abstract：:It still needs to be elucidated whether co-detection of EV71 with other intestinal tract viruses plays a role in the pathogenesis of severe hand-foot-mouth disease (HFMD). A total of 680 stool specimens collected from clinically diagnosed mild and severe-HFMD patients were tested for EV71, CA16, norovirus, bocavirus a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1396-6
更新日期：2012-11-01 00:00:00
abstract：:A full length cDNA copy of the genomic RNA of lettuce mosaic virus (LMV) was constructed under the control of an enhanced CaMV 35S promoter and of the NOS terminator. This construct was found infectious when inoculated to lettuce plants. The intron II of the bean nitrite reductase gene was engineered into the LMV FL c...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050474
更新日期：1998-01-01 00:00:00
abstract：:Genetic analysis of the complete haemagglutinin (HA) gene of fourteen Nigerian avian influenza isolates showed multiple basic amino acids at the cleavage site (321PQRERRRK del R*GLF333), characteristic of highly pathogenic avian influenza (HPAI). Substitution of Gln to Lys at position 322 (H5-specific numbering) was i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3149-4
更新日期：2017-03-01 00:00:00
