Abstract:
:An assay to characterize plasma human immunodeficiency virus 1 (HIV-1) sequences for patients with low viral loads was developed by combining the selective binding of anti-CD44 MicroBeads with a nested RT-PCR targeting the env C2V4 region. Sequences were obtained from 10 of 20 HIV+ patients who had viral loads below 48 copies/ml. Sequences derived from plasma were compared to those from CD14+ CD16 +monocytes and CD4+ T cells. The plasma sequences were most closely related to those amplified from monocytes, suggesting that during successful antiretroviral therapy, the predominant plasma virus originates from myeloid cells. By characterizing HIV-1 RNA sequences from 8 ml of plasma while avoiding multiple steps, which can lead to contamination and deterioration, this method can help elucidate the viral forms in patients with therapeutically suppressed HIV-1. Understanding the source of residual viremia is crucial in developing approaches for viral eradication.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Lopez CA,Vazquez M,Hill MD,Colon Mdel C,Porrata-Doria T,Johnston IC,Lorenzo Edoi
10.1007/s00705-010-0659-3subject
Has Abstractpub_date
2010-06-01 00:00:00pages
895-903issue
6eissn
0304-8608issn
1432-8798journal_volume
155pub_type
杂志文章abstract::As a prerequisite for development of improved vaccines and diagnostic tools for control of the fish pathogen koi herpesvirus, or cyprinid herpesvirus 3 (CyHV-3), we have started to identify putative viral envelope and capsid proteins. The complete or partial CyHV-3 open reading frames ORF25, ORF65, ORF92, ORF99, ORF13...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2190-4
更新日期:2014-12-01 00:00:00
abstract::Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and qua...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1706-7
更新日期:2013-10-01 00:00:00
abstract::We previously reported the isolation of South River virus (SORV) from a pool of mosquitoes collected in the Yucatan Peninsula of Mexico (Farfan-Ale et al. in Vector Borne Zoonotic Dis 10:777-783, 5). The isolate (designated SORV-252) was identified as SORV after a 197-nucleotide region of its small RNA genome segment ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1280-4
更新日期:2012-06-01 00:00:00
abstract::Pseudorabies virus (PRV) is a swine herpesvirus that causes significant morbidity and mortality in swine populations and has caused huge economic losses in the worldwide swine industry. Currently, there is no effective antiviral drug in clinical use for PRV infection; it is also difficult to eliminate PRV from infecte...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3553-4
更新日期:2017-12-01 00:00:00
abstract::A wheat-infecting furovirus found in Tompkins County, New York, U.S.A. was identified as a strain of Soil-borne wheat mosaic virus (SBWMV) by means of sequence analyses of portions of its RNA 1 and 2. The nucleotide sequences of several of its genes differed by c. 9 to 12% from those of the corresponding genome region...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050200011
更新日期:2002-03-01 00:00:00
abstract::The precipitating and neutralizing activities of normal bovine sera with FMDV were studied and compared. Twenty-two out of 79 normal bovine sera gave a positive reaction in micro neutralization tests with FMDV type O, while six did so with type A. In RID tests 32 sera were positive with type O and 28 with type A virus...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315533
更新日期:1978-01-01 00:00:00
abstract::Two independent isolates of an unusual type of rotavirus were obtained from over 900 faecal samples obtained from piglets with diarrhoea. Genome profiles, containing eleven segments, were not characteristic of group A electropherotypes and corresponded to an atypical virus. The profiles were, however, distinct from th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309892
更新日期:1986-01-01 00:00:00
abstract::Cassava mosaic geminiviruses (CMGs) are implicated in cassava mosaic disease (CMD), the main constraint to cassava production in Africa. Here, we report the complete nucleotide sequences of the DNA-A and DNA-B of a newly characterized CMG found infecting cassava in Madagascar, for which we propose the tentative name c...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1399-3
更新日期:2012-10-01 00:00:00
abstract::Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial morphology and dist...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3890-y
更新日期:2018-10-01 00:00:00
abstract::The M protein, encoded by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF6 gene, is considered to be one of the most conserved PRRSV proteins. In recent decades, highly specific monoclonal antibodies (Mabs) have been exploited to provide reliable diagnoses for many diseases. In this study, two diff...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2174-4
更新日期:2014-11-01 00:00:00
abstract::Here, we infected mice with cytopathic bovine viral diarrhea virus 1 (cp BVDV1) by oral inoculation and investigated the effects of infection by histopathological, immunohistochemical (IHC), hematological methods. Twelve mice were infected, and samples were obtained at day 2, 5, and 9 postinfection (pi). Most of the i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2649-y
更新日期:2016-02-01 00:00:00
abstract::The porcine paramyxovirus LPM recognizes alpha, but not beta, anomers of sialic acid containing structures, specifically sialyl (alpha 2,3) lactose. The virus specificity is directed to the sialyl residue and to the C'4 axial OH and the C'6 CH2OH of the galactose present in this structure. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309755
更新日期:1993-01-01 00:00:00
abstract::Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, hum...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0396-7
更新日期:2009-01-01 00:00:00
abstract::Phylogenetic relationships of novel Vietnamese strains of feline immunodeficiency virus (FIV) were analysed. One Vietnamese strain was found to cluster with subtype D, which was previously known only in Japan, while the other seven strains were placed with members of subtype C. Calculation of the relative numbers of m...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0954-8
更新日期:2003-04-01 00:00:00
abstract::We have applied an RNA:RNA hybridization test for the detection of human coronavirus 229E. This test is undergoing further development but already allows a diagnosis of HCV 229E infection within 48 hours. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315554
更新日期:1989-01-01 00:00:00
abstract::Genomic information about Muscovy duck parvovirus is still limited. In this study, the genome of the pathogenic MDPV strain YY was sequenced. The full-length genome of YY is 5075 nucleotides (nt) long, 57 nt shorter than that of strain FM. Sequence alignment indicates that the 5' and 3' inverted terminal repeats (ITR)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2946-0
更新日期:2016-09-01 00:00:00
abstract::Scrapie and Creutzfeldt-Jakob disease (CJD) are caused by infectious agents that are defined phenomenologically. No agent-specific molecules or particles have been identified. Biological properties, such as exponential agent replication and strain variation, as well as physical characteristics of infectivity indicate ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309662
更新日期:1993-01-01 00:00:00
abstract::Heterogeneity of 9 feline herpesvirus type 1 (FHV-1) strains consisting of the prototype C27 strain, one French isolate, six Japanese isolates, and the attenuated vaccine F2 strain was examined by biological, immunological, and molecular biological methods. No significant difference was observed in virus growth and an...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309701
更新日期:1992-01-01 00:00:00
abstract::In order to determine the prevalence of multiple infections with hepatitis viruses in chronic HBsAg carriers in Naples, to assess the interaction between HBV, HDV and HCV infections and to evaluate the influence of multiple virus hepatitis infections on the clinical presentation, we studied 198 HBsAg chronic carriers ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050090
更新日期:1997-01-01 00:00:00
abstract::Swine testicle cell lines were established by transformation of primary swine testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid DNA was transfected into PST cells using a lipofection system. Two related plasmids, pS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310532
更新日期:1990-01-01 00:00:00
abstract::In this study, we obtained the whole genomes of three porcine bocaparvovirus (PBoV) strains (GD6, GD10, and GD23) by polymerase chain reaction. Sequence analysis showed that all three field strains belonged to PBoV group 3 (G3). The phylogenetic trees based on NS1, NP1, and VP1 differed to the extent that these PBoVs ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3606-8
更新日期:2018-03-01 00:00:00
abstract::Mouse hybridoma antibodies against 5 different structural components of measles virus were used in immune fluorescence tests to characterize the appearance of viral antigens in productively infected cell cultures. The antibodies employed in the tests reacted specifically with the hemagglutinin (H, 79K), polymerase (P,...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315171
更新日期:1982-01-01 00:00:00
abstract::H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension cu...
journal_title:Archives of virology
pub_type: 临床试验,杂志文章
doi:10.1007/s00705-017-3460-8
更新日期:2017-10-01 00:00:00
abstract::The nonstructural rotavirus protein NSP1 binds specifically to viral mRNAs and to interferon regulatory factor 3 (IRF3), inducing IRF3 degradation through a proteasome-dependent pathway. By using a vaccinia virus expression system in mammalian cells, we found that the yield of NSP1 was 8- and 13-fold lower than the vi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0850-8
更新日期:2007-02-01 00:00:00
abstract::RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA. Several strategies were explored to use this RNA as a vector for expression of the green fluorescent protein (GFP) in Nicotiana tabaccum plants exp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170125
更新日期:2001-01-01 00:00:00
abstract::We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1625-7
更新日期:2013-07-01 00:00:00
abstract::This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1673-z
更新日期:2013-08-01 00:00:00
abstract::In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of seque...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1141-6
更新日期:2012-01-01 00:00:00
abstract::Seven dsRNA segments were detected from a single Rhizoctonia solani strain HG81. From the full-length cDNA sequences of four smaller dsRNA segments, the genomes of two related partitiviruses, designated as Rhizoctonia solani partitivirus 3 (RsPV3) and RsPV4, were determined. The genomes of RsPV3 and RsPV4 are both com...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3627-3
更新日期:2018-02-01 00:00:00
abstract::The complete nucleotide sequence of the ssRNA genome of a Chinese GPV isolate of barley yellow dwarf virus (BYDV) was determined. It comprised 5673 nucleotides, and the deduced genome organization resembled that of members of the genus Polerovirus. It was most closely related to cereal yellow dwarf virus-RPV (77% nt i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0415-8
更新日期:2009-01-01 00:00:00