Molecular characterization of a canine coronavirus NA/09 strain detected in a dog's organs.

Abstract:

:In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Ntafis V,Xylouri E,Mari V,Papanastassopoulou M,Papaioannou N,Thomas A,Buonavoglia C,Decaro N

doi

10.1007/s00705-011-1141-6

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

171-5

issue

1

eissn

0304-8608

issn

1432-8798

journal_volume

157

pub_type

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