Characterizations of natural and induced polyhedrin gene mutants of Bombyx mori cytoplasmic polyhedrosis viruses.

Abstract:

:Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) polyhedrin gene from natural mutant strain B, B2 and P was cloned and the amino acid sequence was compared with that of wild-type virus (strain H). Chimeric polyhedrin genes (8 types) containing only one site of mutation within the amino acid sequence were also constructed. Fifteen types of polyhedrin genes were introduced into a baculovirus expression vector and hexahedral, acicular, pyramidal, or amorphous polyhedra were formed in infected cells. These results demonstrated that the shape of polyhedra as well as the crystallization pattern of the polyhedrin could be changed by mutations at respectively N-terminal and C-terminal regions of BmCPV polyhedrin gene.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Ikeda K,Nakazawa H,Alain R,Belloncik S,Mori H

doi

10.1007/s007050050283

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

241-8

issue

2

eissn

0304-8608

issn

1432-8798

journal_volume

143

pub_type

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