Experimental infection with cytopathic bovine viral diarrhea virus in mice induces megakaryopoiesis in the spleen and bone marrow.


:Here, we infected mice with cytopathic bovine viral diarrhea virus 1 (cp BVDV1) by oral inoculation and investigated the effects of infection by histopathological, immunohistochemical (IHC), hematological methods. Twelve mice were infected, and samples were obtained at day 2, 5, and 9 postinfection (pi). Most of the infected mice exhibited clinical signs of illness such as reduced movement, crouching, loose feces, loss of appetite, and reduced water intake. Blood samples from six mice were positive for BVDV based on reverse transcription polymerase chain reaction (RT-PCR). Blood analysis also revealed thrombocytopenia and lymphopenia. Viral antigens were detected in the spleen (12/12), bone marrow (12/12), and/or mesenteric lymph nodes (4/12) of all infected mice by IHC analysis. The spleens showed significant histopathological changes including (i) substantially increased numbers of megakaryocytes, (ii) lymphocyte depletion, and (iii) hemorrhages. The bone marrow also had an increased number of megakaryocytes, although this increase was not as strong as it was in the spleen. Severe lymphoid depletion was observed in the mesenteric lymph nodes. Viral infections were present in the lymphocytes but not detected in megakaryocytes of the spleen, bone marrow, or mesenteric lymph nodes. These results suggest that the increased numbers of megakaryocytes may be a direct result of BVDV infection. BVDV infection in mice following oral inoculation of cp BVDV1 leads to megakaryopoiesis in the spleen and bone marrow to replenish the platelets.


Arch Virol


Archives of virology


Seong G,Lee JS,Lee KH,Choi KS




Has Abstract


2016-02-01 00:00:00














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    authors: Gentit P,Foissac X,Svanella-Dumas L,Peypelut M,Candresse T

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    authors: Novikova NA,Morozova OV,Fedorova OF,Epifanova NV,Sashina TA,Efimov EI

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    journal_title:Archives of virology

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    authors: Sheikh M,Bokharaei-Salim F,Monavari SH,Ataei-Pirkooh A,Esghaei M,Moradi N,Babaei R,Fakhim A,Keyvani H

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    authors: Ghildyal R,Baulch-Brown C,Mills J,Meanger J

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    authors: Hashimoto Y,Watanabe A,Kawase S

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    pub_type: 杂志文章


    authors: Fernandes RM,de Lencastre H,Archer LJ

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    authors: La Rosa G,Iaconelli M,Pourshaban M,Fratini M,Muscillo M

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    pub_type: 杂志文章


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    authors: Macdonald RD,Yamamoto T

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    authors: Nishigawa H,Hagiwara T,Yumoto M,Sotome T,Kato T,Natsuaki T

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    pub_type: 杂志文章


    authors: Bukrinskaya AG,Gitelman AK,Martynenko VB

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