Abstract：
:Here, we infected mice with cytopathic bovine viral diarrhea virus 1 (cp BVDV1) by oral inoculation and investigated the effects of infection by histopathological, immunohistochemical (IHC), hematological methods. Twelve mice were infected, and samples were obtained at day 2, 5, and 9 postinfection (pi). Most of the infected mice exhibited clinical signs of illness such as reduced movement, crouching, loose feces, loss of appetite, and reduced water intake. Blood samples from six mice were positive for BVDV based on reverse transcription polymerase chain reaction (RT-PCR). Blood analysis also revealed thrombocytopenia and lymphopenia. Viral antigens were detected in the spleen (12/12), bone marrow (12/12), and/or mesenteric lymph nodes (4/12) of all infected mice by IHC analysis. The spleens showed significant histopathological changes including (i) substantially increased numbers of megakaryocytes, (ii) lymphocyte depletion, and (iii) hemorrhages. The bone marrow also had an increased number of megakaryocytes, although this increase was not as strong as it was in the spleen. Severe lymphoid depletion was observed in the mesenteric lymph nodes. Viral infections were present in the lymphocytes but not detected in megakaryocytes of the spleen, bone marrow, or mesenteric lymph nodes. These results suggest that the increased numbers of megakaryocytes may be a direct result of BVDV infection. BVDV infection in mice following oral inoculation of cp BVDV1 leads to megakaryopoiesis in the spleen and bone marrow to replenish the platelets.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Seong G,Lee JS,Lee KH,Choi KSdoi
10.1007/s00705-015-2649-ysubject
Has Abstractpub_date
2016-02-01 00:00:00pages
417-24issue
2eissn
0304-8608issn
1432-8798pii
10.1007/s00705-015-2649-yjournal_volume
161pub_type
杂志文章abstract：:Peach asteroid spot (PAS) and peach sooty ringspot (PSRS) are two diseases of stone fruit trees of unknown aetiology. The use of a cRNA probe of the newly described Apricot latent virus (ApLV), a tentative member of the Foveavirus genus, indicated the presence of cross-hybridizing agents in PAS isolate LA2 and in PSRS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170071
更新日期：2001-08-01 00:00:00
abstract：:A collection of rotavirus samples collected over a 26-year period was examined to study the dynamics of change in RV strains of genotype P[8] in a geographically defined population (Nizhny Novgorod, Russia; children under 6 years) with no vaccine pressure. Phylogenetic analysis of gene VP4 (subunit VP8*) showed the pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1426-4
更新日期：2012-12-01 00:00:00
abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) causes a variety of cancers, including Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman disease (MCD). Host cellular microRNAs (miRNAs) play important post-transcriptional regulatory roles in gene expression and can greatly influence viru...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3478-y
更新日期：2017-11-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04842-w
更新日期：2020-12-01 00:00:00
abstract：:Occult HCV infection (OCI) has been described as the presence of hepatitis C virus (HCV) genomic RNA in hepatocytes and/or peripheral blood mononuclear cell (PBMC) specimens and the lack of HCV genomic RNA and anti-HCV antibodies (Abs) in plasma samples. Injection drug users (IDUs) are the most important high-risk gro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4066-5
更新日期：2019-02-01 00:00:00
abstract：:In order to investigate the genetic diversity of porcine circovirus type 2 (PCV2), 284 clinical tissue samples were collected from different pig farms in central China from 2015 to 2017. A total of 162 tissue samples (162/284, 57.04%) were positive for PCV2 by PCR, and subsequently, the complete genome of 36 of these ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04205-0
更新日期：2019-05-01 00:00:00
abstract：:A flow cytometric virus binding assay that directly visualizes the binding of infectious bursal disease virus (IBDV) to its target cells was established. The chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for IBDV infection, bound high levels of the virus. Another B lymphoblastoid cell line, LSCC-11...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050464
更新日期：1998-01-01 00:00:00
abstract：:Herpes simplex virus type 2 (HSV-2), strain YS-4 C-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (ACV; acycloguanosine) in vitro. It was sensitive to phosphonoacetic acid and 9-beta-D-arabinofuranosyladenine. Thymidine kinase (TK) activity of YS-4 C-1 was less than 1% of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310998
更新日期：1988-01-01 00:00:00
abstract：:We studied the kinetics of localisation of matrix (M) protein of Human respiratory syncytial virus (RSV) in infected cells. M protein was detected in the nucleus early in infection, by confocal microscopy and by immunoblotting of nuclear fractions. We next tested the possibility that M protein may be involved in inhib...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0112-y
更新日期：2003-07-01 00:00:00
abstract：:The viral erythrocytic infection (VEI) of sea bass (Dicentrarchus labrax) is evidenced by the presence of inclusion bodies in the erythrocytes and erythroblasts. Virus-like particles (VEIV) about 135-150 nm in diameter were observed in erythroblasts of affected fish. No culturable virus could be recovered from the org...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310951
更新日期：1991-01-01 00:00:00
abstract：:Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
abstract：:To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishmen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0926-z
更新日期：2003-02-01 00:00:00
abstract：:This study describes surveillance for avian influenza viruses (AIV) in the Minto Flats State Game Refuge, high-density waterfowl breeding grounds in Alaska. Five hundred paired cloacal samples from dabbling ducks (Northern Pintail, Mallard, Green Wing Teal, and Widgeon) were placed into ethanol and viral transport med...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0994-1
更新日期：2007-01-01 00:00:00
abstract：:The Karens, or 'old settlers', migrated from Myanmar to Andaman and Nicobar islands 80 years ago. A high HBV exposure rate among them has been reported. A study of 34 HBsAg carriers was done to investigate the origin of HBV infection among the Karens. RFLP-based genotyping was confirmed by sequencing and phylogenetic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0910-0
更新日期：2007-01-01 00:00:00
abstract：:Mengovirus, extracted from infected L-cell cultures with Freon 113 and concentrated from the aqueous phase with polyethyleneglycol, was chromatographed on protein-coated controlled pore glass (CPG). The covalent binding of protein to CPG is described. Further purification and concentration of mengovirus was achieved b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315283
更新日期：1978-01-01 00:00:00
abstract：:vp1, a gene encoding one of the capsid proteins of Taura syndrome virus, was cloned into the pGEX-6P-1 expression vector, and the resulting construct was then used to transform E. coli strain BL21. After induction, an N-terminally glutathione-S-transferase-tagged VP1 (GST-VP1) protein with a molecular mass of 80 kDa w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1460-2
更新日期：2013-01-01 00:00:00
abstract：:Human adenoviruses (HAdV) of species B, C, and E (HAdV-B, -C, -E) are frequent causative agents of acute respiratory infections worldwide. No specific analysis has been done on the epidemiological and clinical features of HAdV in pediatric pneumonia in China. Nasopharyngeal aspirates were collected from hospitalized c...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2308-8
更新日期：2015-03-01 00:00:00
abstract：:Complete nucleotide sequences of cytoplasmic inclusion (CI) genes of two strains of potato virus Y (PVY) were determined from six polymerase chain reaction (PCR)-amplified cDNA clones. The size of the CI genes of both ordinary (PVY-O) and necrotic strains (PVY-T13) was 1902 nucleotides, with a sequence homology of 83....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309785
更新日期：1993-01-01 00:00:00
abstract：:Hepatitis E represents an important public-health concern throughout the world. It is one of the leading causes of hepatitis in North Africa, Asia and the Middle East. In Tunisia, the true burden of HEV infection is still unknown. The objectives of the present study were to assess the occurrence of hepatitis E virus i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2251-8
更新日期：2015-01-01 00:00:00
abstract：:Two non-virogenic Vero cell lines persistently infected with the arenavirus Junin (JUNV), named V3 and V7, were characterized with respect to their resistance to superinfection with homologous and antigenically related viruses. Both lines were refractory to JUNV multiplication and partially resistant to other arenavir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0227-1
更新日期：2004-03-01 00:00:00
abstract：:Reassortants between a low-pathogenic avian influenza virus strain A/Duck/Primorie/2621/2001 (H5N2) and a high-yield human influenza virus strain A/Puerto Rico/8/34 (H1N1) were generated, genotyped and analyzed with respect to their yield in embryonated chicken eggs, pathogenicity for mice, and immunogenicity. A reass...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0931-8
更新日期：2007-01-01 00:00:00
abstract：:DNA of TT virus (TTV), a novel human circovirus, was tested for in 116 mother-infant pairs who had participated in the adult T-cell leukemia prevention program (APP) in Nagasaki, Japan, and refrained from breast-feeding. By polymerase chain reaction with Okamoto's seminested primers, 36 of the 115 (31%) mothers were p...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050044
更新日期：2000-01-01 00:00:00
abstract：:The genomic RNA of infectious flacherie virus (IFV), an insect picornavirus, was shown to contain a covalently attached substance which can be labeled with 125I-Bolton and Hunter reagent. This substance was found to be a protein covalently linked to the virus genome, because it cosedimented and was copurified with the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317378
更新日期：1986-01-01 00:00:00
abstract：:Infectious cDNA clones originally derived from a mild strain of Pepper mild mottle virus were constructed by replacing residue 649, a critical point for attenuation of this virus, with all possible amino acids. All clones were infectious to pepper plants and induced a variety of symptoms, including no visible symptoms...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0312-1
更新日期：2009-01-01 00:00:00
abstract：:IG 1, a temperate phage of Bacillus subtilis, is strongly induced from its lysogens by 6-(p-hydroxyphenylazo)-uracil, an azopyrimidine known as selectively inhibiting the B. subtilis DNA polymerase III. IG 1 phages originated either by induction or infection multiply, abundantly, in the presence of that azopyrimidine,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316671
更新日期：1990-01-01 00:00:00
abstract：:Noroviruses cause acute viral gastroenteritis worldwide. They are classified in five genogroups, of which GI, GII, and GIV infect humans. Little information is available on the prevalence and clinical effects of GIV noroviruses. We conducted a large-scale molecular-epidemiological investigation, a yearlong monitoring ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0619-y
更新日期：2010-04-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) has evolved several immune-evasion strategies. One strategy involves encoding Fcgamma receptor (FcγR)-like proteins that thwart the Fcγ-mediated effector functions. Our aim was to determine whether GM allotypes-immunoglobulin γ chain determinants expressed primarily on the Fc segment-modul...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0937-8
更新日期：2011-05-01 00:00:00
abstract：:Infectious pancreatic necrosis virus exhibited an interference phenomenon that resulted in the survival of the infected cell with one hit kinetics. The responsible factor was found to co-purify with standard virus through a purification regime that employed two CsCl gradients and a sucrose gradient. This result sugges...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315639
更新日期：1978-01-01 00:00:00
abstract：:Complete nucleotide sequences of three strains (I, III, and IV) of Barley yellow mosaic virus (BaYMV) isolated in Japan were determined. The length of the genome was the same among the three strains; RNA1 was 7,642 nt and RNA2 was 3,585 nt. The molecular phylogenetic analysis showed that strain I was most closely rela...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0163-1
更新日期：2008-01-01 00:00:00
abstract：:Noninfectious virus particles were produced in Ehrlich ascites tumor cells infected intraperitoneally with fowl plague virus. The PFU yield of virus per cell was less than 0.1 and the ratio PFU/HA units in the progeny virus was less than 10(3). The virus particles had the same morphology and size as egg-grown virus bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315278
更新日期：1978-01-01 00:00:00
